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Na,K-ATPase subunit beta1 knock-in prevents lethality of beta2 deficiency in mice

Standard

Na,K-ATPase subunit beta1 knock-in prevents lethality of beta2 deficiency in mice. / Weber, P; Bartsch, U; Schachner, M; Montag, D.

in: J NEUROSCI, Jahrgang 18, Nr. 22, 15.11.1998, S. 9192-203.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Weber, P, Bartsch, U, Schachner, M & Montag, D 1998, 'Na,K-ATPase subunit beta1 knock-in prevents lethality of beta2 deficiency in mice', J NEUROSCI, Jg. 18, Nr. 22, S. 9192-203.

APA

Weber, P., Bartsch, U., Schachner, M., & Montag, D. (1998). Na,K-ATPase subunit beta1 knock-in prevents lethality of beta2 deficiency in mice. J NEUROSCI, 18(22), 9192-203.

Vancouver

Weber P, Bartsch U, Schachner M, Montag D. Na,K-ATPase subunit beta1 knock-in prevents lethality of beta2 deficiency in mice. J NEUROSCI. 1998 Nov 15;18(22):9192-203.

Bibtex

@article{16a191ddb48d4f588f0f393545ddbfaa,
title = "Na,K-ATPase subunit beta1 knock-in prevents lethality of beta2 deficiency in mice",
abstract = "The beta2 subunit of the Na,K-ATPase displays functional properties of both an integral constituent of an ion pump and an adhesion and neurite outgrowth-promoting molecule in vitro. To investigate whether the beta1 subunit of the Na,K-ATPase can functionally substitute for the beta2 isoform in vivo, we have generated beta2/beta1 knock-in mice by homologous recombination in embryonic stem cells. In beta2/beta1 knock-in mice, expression of beta2 was abolished, whereas beta1 mRNA expression from the mutated gene amounted to approximately 15% of the normal expression of beta2 in the adult mouse brain and prevented the juvenile lethality observed for beta2 null mutant mice. In contrast to beta2 null mutant mice, the overall morphological structure of all analyzed brain regions was normal. By immunohistochemical analysis, beta1 expression was detected in photoreceptor cells in the retina of knock-in mice at an age when expression of beta1 and beta2, respectively, is downregulated and persisting in the wild-type mice. Morphological analysis by light and electron microscopy revealed a progressive degeneration of photoreceptor cells. Apoptotic death of photoreceptor cells determined quantitatively by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling analysis increased in beta2/beta1 knock-in mice with age. These observations suggest that the beta1 subunit of the Na,K-ATPase can substitute sufficiently, at least in certain cell types, for the role of the beta2 subunit as a component of a functional Na,K-ATPase, but they do not allow us to determine the possible role of the beta2 subunit as an adhesion molecule in vivo.",
keywords = "Amino Acid Sequence, Animals, Apoptosis, Cell Adhesion Molecules, Homeostasis, Immunohistochemistry, In Situ Nick-End Labeling, Mice, Mice, Mutant Strains, Microscopy, Electron, Molecular Sequence Data, Mutagenesis, Neuroglia, Phenotype, Photoreceptor Cells, Vertebrate, Retinitis Pigmentosa, Sodium-Potassium-Exchanging ATPase, Stem Cells, Journal Article",
author = "P Weber and U Bartsch and M Schachner and D Montag",
year = "1998",
month = nov,
day = "15",
language = "English",
volume = "18",
pages = "9192--203",
journal = "J NEUROSCI",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "22",

}

RIS

TY - JOUR

T1 - Na,K-ATPase subunit beta1 knock-in prevents lethality of beta2 deficiency in mice

AU - Weber, P

AU - Bartsch, U

AU - Schachner, M

AU - Montag, D

PY - 1998/11/15

Y1 - 1998/11/15

N2 - The beta2 subunit of the Na,K-ATPase displays functional properties of both an integral constituent of an ion pump and an adhesion and neurite outgrowth-promoting molecule in vitro. To investigate whether the beta1 subunit of the Na,K-ATPase can functionally substitute for the beta2 isoform in vivo, we have generated beta2/beta1 knock-in mice by homologous recombination in embryonic stem cells. In beta2/beta1 knock-in mice, expression of beta2 was abolished, whereas beta1 mRNA expression from the mutated gene amounted to approximately 15% of the normal expression of beta2 in the adult mouse brain and prevented the juvenile lethality observed for beta2 null mutant mice. In contrast to beta2 null mutant mice, the overall morphological structure of all analyzed brain regions was normal. By immunohistochemical analysis, beta1 expression was detected in photoreceptor cells in the retina of knock-in mice at an age when expression of beta1 and beta2, respectively, is downregulated and persisting in the wild-type mice. Morphological analysis by light and electron microscopy revealed a progressive degeneration of photoreceptor cells. Apoptotic death of photoreceptor cells determined quantitatively by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling analysis increased in beta2/beta1 knock-in mice with age. These observations suggest that the beta1 subunit of the Na,K-ATPase can substitute sufficiently, at least in certain cell types, for the role of the beta2 subunit as a component of a functional Na,K-ATPase, but they do not allow us to determine the possible role of the beta2 subunit as an adhesion molecule in vivo.

AB - The beta2 subunit of the Na,K-ATPase displays functional properties of both an integral constituent of an ion pump and an adhesion and neurite outgrowth-promoting molecule in vitro. To investigate whether the beta1 subunit of the Na,K-ATPase can functionally substitute for the beta2 isoform in vivo, we have generated beta2/beta1 knock-in mice by homologous recombination in embryonic stem cells. In beta2/beta1 knock-in mice, expression of beta2 was abolished, whereas beta1 mRNA expression from the mutated gene amounted to approximately 15% of the normal expression of beta2 in the adult mouse brain and prevented the juvenile lethality observed for beta2 null mutant mice. In contrast to beta2 null mutant mice, the overall morphological structure of all analyzed brain regions was normal. By immunohistochemical analysis, beta1 expression was detected in photoreceptor cells in the retina of knock-in mice at an age when expression of beta1 and beta2, respectively, is downregulated and persisting in the wild-type mice. Morphological analysis by light and electron microscopy revealed a progressive degeneration of photoreceptor cells. Apoptotic death of photoreceptor cells determined quantitatively by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling analysis increased in beta2/beta1 knock-in mice with age. These observations suggest that the beta1 subunit of the Na,K-ATPase can substitute sufficiently, at least in certain cell types, for the role of the beta2 subunit as a component of a functional Na,K-ATPase, but they do not allow us to determine the possible role of the beta2 subunit as an adhesion molecule in vivo.

KW - Amino Acid Sequence

KW - Animals

KW - Apoptosis

KW - Cell Adhesion Molecules

KW - Homeostasis

KW - Immunohistochemistry

KW - In Situ Nick-End Labeling

KW - Mice

KW - Mice, Mutant Strains

KW - Microscopy, Electron

KW - Molecular Sequence Data

KW - Mutagenesis

KW - Neuroglia

KW - Phenotype

KW - Photoreceptor Cells, Vertebrate

KW - Retinitis Pigmentosa

KW - Sodium-Potassium-Exchanging ATPase

KW - Stem Cells

KW - Journal Article

M3 - SCORING: Journal article

C2 - 9801359

VL - 18

SP - 9192

EP - 9203

JO - J NEUROSCI

JF - J NEUROSCI

SN - 0270-6474

IS - 22

ER -