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Myogenic differentiation of mesenchymal stem cells co-cultured with primary myoblasts.

Standard

Myogenic differentiation of mesenchymal stem cells co-cultured with primary myoblasts. / Beier, Justus P; Bitto, Franz F; Lange, Claudia; Klumpp, Dorothee; Arkudas, Andreas; Bleiziffer, Oliver; Boos, Anja M; Horch, Raymund E; Kneser, Ulrich.

in: CELL BIOL INT, Jahrgang 35, Nr. 4, 4, 2011, S. 397-406.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Beier, JP, Bitto, FF, Lange, C, Klumpp, D, Arkudas, A, Bleiziffer, O, Boos, AM, Horch, RE & Kneser, U 2011, 'Myogenic differentiation of mesenchymal stem cells co-cultured with primary myoblasts.', CELL BIOL INT, Jg. 35, Nr. 4, 4, S. 397-406. <http://www.ncbi.nlm.nih.gov/pubmed/20946104?dopt=Citation>

APA

Beier, J. P., Bitto, F. F., Lange, C., Klumpp, D., Arkudas, A., Bleiziffer, O., Boos, A. M., Horch, R. E., & Kneser, U. (2011). Myogenic differentiation of mesenchymal stem cells co-cultured with primary myoblasts. CELL BIOL INT, 35(4), 397-406. [4]. http://www.ncbi.nlm.nih.gov/pubmed/20946104?dopt=Citation

Vancouver

Beier JP, Bitto FF, Lange C, Klumpp D, Arkudas A, Bleiziffer O et al. Myogenic differentiation of mesenchymal stem cells co-cultured with primary myoblasts. CELL BIOL INT. 2011;35(4):397-406. 4.

Bibtex

@article{725c979a3bdd4a4893e209057f4da2f5,
title = "Myogenic differentiation of mesenchymal stem cells co-cultured with primary myoblasts.",
abstract = "TE (tissue engineering) of skeletal muscle is a promising method to reconstruct loss of muscle tissue. This study evaluates MSCs (mesenchymal stem cells) as new cell source for this application. As a new approach to differentiate the MSCs towards the myogenic lineage, co-cultivation with primary myoblasts has been developed and the myogenic potential of GFP (green fluorescent protein)-transduced rat MSC co-cultured with primary rat myoblasts was assessed by ICC (immunocytochemistry). Myogenic potential of MSC was analysed by ICC, FACS and qPCR (quantitative PCR). MSC-myoblast fusion phenomena leading to hybrid myotubes were evaluated using a novel method to evaluate myotube fusion ratios based on phase contrast and fluorescence microscopy. Furthermore, MSC constitutively expressed the myogenic markers MEF2 (myogenic enhancer factor 2) and ?-sarcomeric actin, and MEF2 expression was up-regulated upon co-cultivation with primary myoblasts and the addition of myogenic medium supplements. Significantly higher numbers of MSC nuclei were involved in myotube formations when bFGF (basic fibroblast growth factor) and dexamethasone were added to co-cultures. In summary, we have determined optimal co-culture conditions for MSC myogenic differentiation up to myotube formations as a promising step towards applicability of MSC as a cell source for skeletal muscle TE as well as other muscle cell-based therapies.",
keywords = "Animals, Male, Cells, Cultured, Rats, Cell Line, Tissue Engineering/methods, *Cell Differentiation, Coculture Techniques/*methods, Mesenchymal Stem Cells/*cytology, Myoblasts/*cytology, Rats, Inbred Lew, Animals, Male, Cells, Cultured, Rats, Cell Line, Tissue Engineering/methods, *Cell Differentiation, Coculture Techniques/*methods, Mesenchymal Stem Cells/*cytology, Myoblasts/*cytology, Rats, Inbred Lew",
author = "Beier, {Justus P} and Bitto, {Franz F} and Claudia Lange and Dorothee Klumpp and Andreas Arkudas and Oliver Bleiziffer and Boos, {Anja M} and Horch, {Raymund E} and Ulrich Kneser",
year = "2011",
language = "English",
volume = "35",
pages = "397--406",
journal = "CELL BIOL INT",
issn = "1065-6995",
publisher = "PORTLAND PRESS LTD",
number = "4",

}

RIS

TY - JOUR

T1 - Myogenic differentiation of mesenchymal stem cells co-cultured with primary myoblasts.

AU - Beier, Justus P

AU - Bitto, Franz F

AU - Lange, Claudia

AU - Klumpp, Dorothee

AU - Arkudas, Andreas

AU - Bleiziffer, Oliver

AU - Boos, Anja M

AU - Horch, Raymund E

AU - Kneser, Ulrich

PY - 2011

Y1 - 2011

N2 - TE (tissue engineering) of skeletal muscle is a promising method to reconstruct loss of muscle tissue. This study evaluates MSCs (mesenchymal stem cells) as new cell source for this application. As a new approach to differentiate the MSCs towards the myogenic lineage, co-cultivation with primary myoblasts has been developed and the myogenic potential of GFP (green fluorescent protein)-transduced rat MSC co-cultured with primary rat myoblasts was assessed by ICC (immunocytochemistry). Myogenic potential of MSC was analysed by ICC, FACS and qPCR (quantitative PCR). MSC-myoblast fusion phenomena leading to hybrid myotubes were evaluated using a novel method to evaluate myotube fusion ratios based on phase contrast and fluorescence microscopy. Furthermore, MSC constitutively expressed the myogenic markers MEF2 (myogenic enhancer factor 2) and ?-sarcomeric actin, and MEF2 expression was up-regulated upon co-cultivation with primary myoblasts and the addition of myogenic medium supplements. Significantly higher numbers of MSC nuclei were involved in myotube formations when bFGF (basic fibroblast growth factor) and dexamethasone were added to co-cultures. In summary, we have determined optimal co-culture conditions for MSC myogenic differentiation up to myotube formations as a promising step towards applicability of MSC as a cell source for skeletal muscle TE as well as other muscle cell-based therapies.

AB - TE (tissue engineering) of skeletal muscle is a promising method to reconstruct loss of muscle tissue. This study evaluates MSCs (mesenchymal stem cells) as new cell source for this application. As a new approach to differentiate the MSCs towards the myogenic lineage, co-cultivation with primary myoblasts has been developed and the myogenic potential of GFP (green fluorescent protein)-transduced rat MSC co-cultured with primary rat myoblasts was assessed by ICC (immunocytochemistry). Myogenic potential of MSC was analysed by ICC, FACS and qPCR (quantitative PCR). MSC-myoblast fusion phenomena leading to hybrid myotubes were evaluated using a novel method to evaluate myotube fusion ratios based on phase contrast and fluorescence microscopy. Furthermore, MSC constitutively expressed the myogenic markers MEF2 (myogenic enhancer factor 2) and ?-sarcomeric actin, and MEF2 expression was up-regulated upon co-cultivation with primary myoblasts and the addition of myogenic medium supplements. Significantly higher numbers of MSC nuclei were involved in myotube formations when bFGF (basic fibroblast growth factor) and dexamethasone were added to co-cultures. In summary, we have determined optimal co-culture conditions for MSC myogenic differentiation up to myotube formations as a promising step towards applicability of MSC as a cell source for skeletal muscle TE as well as other muscle cell-based therapies.

KW - Animals

KW - Male

KW - Cells, Cultured

KW - Rats

KW - Cell Line

KW - Tissue Engineering/methods

KW - Cell Differentiation

KW - Coculture Techniques/methods

KW - Mesenchymal Stem Cells/cytology

KW - Myoblasts/cytology

KW - Rats, Inbred Lew

KW - Animals

KW - Male

KW - Cells, Cultured

KW - Rats

KW - Cell Line

KW - Tissue Engineering/methods

KW - Cell Differentiation

KW - Coculture Techniques/methods

KW - Mesenchymal Stem Cells/cytology

KW - Myoblasts/cytology

KW - Rats, Inbred Lew

M3 - SCORING: Journal article

VL - 35

SP - 397

EP - 406

JO - CELL BIOL INT

JF - CELL BIOL INT

SN - 1065-6995

IS - 4

M1 - 4

ER -