PortalPublikationenMyeloperoxidase is required for neutrophil extracellular tra...

Myeloperoxidase is required for neutrophil extracellular trap formation

Standard

Myeloperoxidase is required for neutrophil extracellular trap formation : implications for innate immunity. / Metzler, Kathleen D; Fuchs, Tobias A; Nauseef, William M; Reumaux, Dominique; Roesler, Joachim; Schulze, Ilka; Wahn, Volker; Papayannopoulos, Venizelos; Zychlinsky, Arturo.

in: BLOOD, Jahrgang 117, Nr. 3, 20.01.2011, S. 953-9.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Metzler, KD, Fuchs, TA, Nauseef, WM, Reumaux, D, Roesler, J, Schulze, I, Wahn, V, Papayannopoulos, V & Zychlinsky, A 2011, 'Myeloperoxidase is required for neutrophil extracellular trap formation: implications for innate immunity', BLOOD, Jg. 117, Nr. 3, S. 953-9. https://doi.org/10.1182/blood-2010-06-290171

APA

Metzler, K. D., Fuchs, T. A., Nauseef, W. M., Reumaux, D., Roesler, J., Schulze, I., Wahn, V., Papayannopoulos, V., & Zychlinsky, A. (2011). Myeloperoxidase is required for neutrophil extracellular trap formation: implications for innate immunity. BLOOD, 117(3), 953-9. https://doi.org/10.1182/blood-2010-06-290171

Vancouver

Metzler KD, Fuchs TA, Nauseef WM, Reumaux D, Roesler J, Schulze I et al. Myeloperoxidase is required for neutrophil extracellular trap formation: implications for innate immunity. BLOOD. 2011 Jan 20;117(3):953-9. https://doi.org/10.1182/blood-2010-06-290171

Bibtex

@article{cb77e3cafa9a458f8f10d0877aa5aa14,
title = "Myeloperoxidase is required for neutrophil extracellular trap formation: implications for innate immunity",
abstract = "The granule enzyme myeloperoxidase (MPO) plays an important role in neutrophil antimicrobial responses. However, the severity of immunodeficiency in patients carrying mutations in MPO is variable. Serious microbial infections, especially with Candida species, have been observed in a subset of completely MPO-deficient patients. Here we show that neutrophils from donors who are completely deficient in MPO fail to form neutrophil extracellular traps (NETs), indicating that MPO is required for NET formation. In contrast, neutrophils from partially MPO-deficient donors make NETs, and pharmacological inhibition of MPO only delays and reduces NET formation. Extracellular products of MPO do not rescue NET formation, suggesting that MPO acts cell-autonomously. Finally, NET-dependent inhibition of Candida albicans growth is compromised in MPO-deficient neutrophils. The inability to form NETs may contribute in part to the host defense defects observed in completely MPO-deficient individuals.",
keywords = "Aniline Compounds, Blood Donors, Blotting, Western, Candida albicans, Cells, Cultured, Extracellular Space, Genotype, Host-Pathogen Interactions, Humans, Immunity, Innate, Microscopy, Fluorescence, Mutation, Neutrophils, Peroxidase, Tetradecanoylphorbol Acetate",
author = "Metzler, {Kathleen D} and Fuchs, {Tobias A} and Nauseef, {William M} and Dominique Reumaux and Joachim Roesler and Ilka Schulze and Volker Wahn and Venizelos Papayannopoulos and Arturo Zychlinsky",
year = "2011",
month = jan,
day = "20",
doi = "10.1182/blood-2010-06-290171",
language = "English",
volume = "117",
pages = "953--9",
journal = "BLOOD",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "3",

}

RIS

TY - JOUR

T1 - Myeloperoxidase is required for neutrophil extracellular trap formation

T2 - implications for innate immunity

AU - Metzler, Kathleen D

AU - Fuchs, Tobias A

AU - Nauseef, William M

AU - Reumaux, Dominique

AU - Roesler, Joachim

AU - Schulze, Ilka

AU - Wahn, Volker

AU - Papayannopoulos, Venizelos

AU - Zychlinsky, Arturo

PY - 2011/1/20

Y1 - 2011/1/20

N2 - The granule enzyme myeloperoxidase (MPO) plays an important role in neutrophil antimicrobial responses. However, the severity of immunodeficiency in patients carrying mutations in MPO is variable. Serious microbial infections, especially with Candida species, have been observed in a subset of completely MPO-deficient patients. Here we show that neutrophils from donors who are completely deficient in MPO fail to form neutrophil extracellular traps (NETs), indicating that MPO is required for NET formation. In contrast, neutrophils from partially MPO-deficient donors make NETs, and pharmacological inhibition of MPO only delays and reduces NET formation. Extracellular products of MPO do not rescue NET formation, suggesting that MPO acts cell-autonomously. Finally, NET-dependent inhibition of Candida albicans growth is compromised in MPO-deficient neutrophils. The inability to form NETs may contribute in part to the host defense defects observed in completely MPO-deficient individuals.

AB - The granule enzyme myeloperoxidase (MPO) plays an important role in neutrophil antimicrobial responses. However, the severity of immunodeficiency in patients carrying mutations in MPO is variable. Serious microbial infections, especially with Candida species, have been observed in a subset of completely MPO-deficient patients. Here we show that neutrophils from donors who are completely deficient in MPO fail to form neutrophil extracellular traps (NETs), indicating that MPO is required for NET formation. In contrast, neutrophils from partially MPO-deficient donors make NETs, and pharmacological inhibition of MPO only delays and reduces NET formation. Extracellular products of MPO do not rescue NET formation, suggesting that MPO acts cell-autonomously. Finally, NET-dependent inhibition of Candida albicans growth is compromised in MPO-deficient neutrophils. The inability to form NETs may contribute in part to the host defense defects observed in completely MPO-deficient individuals.

KW - Aniline Compounds

KW - Blood Donors

KW - Blotting, Western

KW - Candida albicans

KW - Cells, Cultured

KW - Extracellular Space

KW - Genotype

KW - Host-Pathogen Interactions

KW - Humans

KW - Immunity, Innate

KW - Microscopy, Fluorescence

KW - Mutation

KW - Neutrophils

KW - Peroxidase

KW - Tetradecanoylphorbol Acetate

U2 - 10.1182/blood-2010-06-290171

DO - 10.1182/blood-2010-06-290171

M3 - SCORING: Journal article

C2 - 20974672

VL - 117

SP - 953

EP - 959

JO - BLOOD

JF - BLOOD

SN - 0006-4971

IS - 3

ER -