PortalPublikationenMyeloid cell function in MRP-14 (S100A9) null mice

Myeloid cell function in MRP-14 (S100A9) null mice

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Myeloid cell function in MRP-14 (S100A9) null mice. / Hobbs, Josie A R; May, Richard; Tanousis, Kiki; McNeill, Eileen; Mathies, Margaret; Gebhardt, Christoffer; Henderson, Robert; Robinson, Matthew J; Hogg, Nancy.

in: MOL CELL BIOL, Jahrgang 23, Nr. 7, 04.2003, S. 2564-76.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Hobbs, JAR, May, R, Tanousis, K, McNeill, E, Mathies, M, Gebhardt, C, Henderson, R, Robinson, MJ & Hogg, N 2003, 'Myeloid cell function in MRP-14 (S100A9) null mice', MOL CELL BIOL, Jg. 23, Nr. 7, S. 2564-76.

APA

Hobbs, J. A. R., May, R., Tanousis, K., McNeill, E., Mathies, M., Gebhardt, C., Henderson, R., Robinson, M. J., & Hogg, N. (2003). Myeloid cell function in MRP-14 (S100A9) null mice. MOL CELL BIOL, 23(7), 2564-76.

Vancouver

Hobbs JAR, May R, Tanousis K, McNeill E, Mathies M, Gebhardt C et al. Myeloid cell function in MRP-14 (S100A9) null mice. MOL CELL BIOL. 2003 Apr;23(7):2564-76.

Bibtex

@article{cac8ee596451430b859632ff64af52e5,
title = "Myeloid cell function in MRP-14 (S100A9) null mice",
abstract = "Myeloid-related protein 14 (MRP-14) and its heterodimeric partner, MRP-8, are cytosolic calcium-binding proteins, highly expressed in neutrophils and monocytes. To understand the function of MRP-14, we performed targeted disruption of the MRP-14 gene in mice. MRP-14(-/-) mice showed no obvious phenotype and were fertile. MRP-8 mRNA but not protein is present in the myeloid cells of these mice, suggesting that the stability of MRP-8 protein is dependent on MRP-14 expression. A compensatory increase in other proteins was not detected in cells lacking MRP-8 and MRP-14. Although the morphology of MRP-14(-/-) myeloid cells was not altered, they were significantly less dense. When Ca(2+) responses were investigated, there was no change in the maximal response to the chemokine MIP-2. At lower concentrations, however, there was reduced responsiveness in MRP-14(-/-) compared with MRP-14(+/+) neutrophils. This alteration in the ability to flux Ca(2+) did not impair the ability of the MRP-14(-/-) neutrophils to respond chemotactically to MIP-2. In addition, the myeloid cell functions of phagocytosis, superoxide burst, and apoptosis were unaffected in MRP-14(-/-) cells. In an in vivo model of peritonitis, MRP-14(-/-) mice showed no difference from wild-type mice in induced inflammatory response. The data indicate that MRP-14 and MRP-8 are dispensable for many myeloid cell functions.",
keywords = "Animals, Calcium, Calgranulin A, Calgranulin B, Cell Count, Cells, Cultured, Chemokine CXCL2, Chemokines, Dose-Response Relationship, Drug, Fluorescent Dyes, Gene Expression Regulation, Developmental, Gene Targeting, Mice, Mice, Knockout, Monocytes, Myeloid Cells, Neutrophils, Peritonitis, Phenotype, RNA, Messenger, Journal Article, Research Support, Non-U.S. Gov't",
author = "Hobbs, {Josie A R} and Richard May and Kiki Tanousis and Eileen McNeill and Margaret Mathies and Christoffer Gebhardt and Robert Henderson and Robinson, {Matthew J} and Nancy Hogg",
year = "2003",
month = apr,
language = "English",
volume = "23",
pages = "2564--76",
journal = "MOL CELL BIOL",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "7",

}

RIS

TY - JOUR

T1 - Myeloid cell function in MRP-14 (S100A9) null mice

AU - Hobbs, Josie A R

AU - May, Richard

AU - Tanousis, Kiki

AU - McNeill, Eileen

AU - Mathies, Margaret

AU - Gebhardt, Christoffer

AU - Henderson, Robert

AU - Robinson, Matthew J

AU - Hogg, Nancy

PY - 2003/4

Y1 - 2003/4

N2 - Myeloid-related protein 14 (MRP-14) and its heterodimeric partner, MRP-8, are cytosolic calcium-binding proteins, highly expressed in neutrophils and monocytes. To understand the function of MRP-14, we performed targeted disruption of the MRP-14 gene in mice. MRP-14(-/-) mice showed no obvious phenotype and were fertile. MRP-8 mRNA but not protein is present in the myeloid cells of these mice, suggesting that the stability of MRP-8 protein is dependent on MRP-14 expression. A compensatory increase in other proteins was not detected in cells lacking MRP-8 and MRP-14. Although the morphology of MRP-14(-/-) myeloid cells was not altered, they were significantly less dense. When Ca(2+) responses were investigated, there was no change in the maximal response to the chemokine MIP-2. At lower concentrations, however, there was reduced responsiveness in MRP-14(-/-) compared with MRP-14(+/+) neutrophils. This alteration in the ability to flux Ca(2+) did not impair the ability of the MRP-14(-/-) neutrophils to respond chemotactically to MIP-2. In addition, the myeloid cell functions of phagocytosis, superoxide burst, and apoptosis were unaffected in MRP-14(-/-) cells. In an in vivo model of peritonitis, MRP-14(-/-) mice showed no difference from wild-type mice in induced inflammatory response. The data indicate that MRP-14 and MRP-8 are dispensable for many myeloid cell functions.

AB - Myeloid-related protein 14 (MRP-14) and its heterodimeric partner, MRP-8, are cytosolic calcium-binding proteins, highly expressed in neutrophils and monocytes. To understand the function of MRP-14, we performed targeted disruption of the MRP-14 gene in mice. MRP-14(-/-) mice showed no obvious phenotype and were fertile. MRP-8 mRNA but not protein is present in the myeloid cells of these mice, suggesting that the stability of MRP-8 protein is dependent on MRP-14 expression. A compensatory increase in other proteins was not detected in cells lacking MRP-8 and MRP-14. Although the morphology of MRP-14(-/-) myeloid cells was not altered, they were significantly less dense. When Ca(2+) responses were investigated, there was no change in the maximal response to the chemokine MIP-2. At lower concentrations, however, there was reduced responsiveness in MRP-14(-/-) compared with MRP-14(+/+) neutrophils. This alteration in the ability to flux Ca(2+) did not impair the ability of the MRP-14(-/-) neutrophils to respond chemotactically to MIP-2. In addition, the myeloid cell functions of phagocytosis, superoxide burst, and apoptosis were unaffected in MRP-14(-/-) cells. In an in vivo model of peritonitis, MRP-14(-/-) mice showed no difference from wild-type mice in induced inflammatory response. The data indicate that MRP-14 and MRP-8 are dispensable for many myeloid cell functions.

KW - Animals

KW - Calcium

KW - Calgranulin A

KW - Calgranulin B

KW - Cell Count

KW - Cells, Cultured

KW - Chemokine CXCL2

KW - Chemokines

KW - Dose-Response Relationship, Drug

KW - Fluorescent Dyes

KW - Gene Expression Regulation, Developmental

KW - Gene Targeting

KW - Mice

KW - Mice, Knockout

KW - Monocytes

KW - Myeloid Cells

KW - Neutrophils

KW - Peritonitis

KW - Phenotype

KW - RNA, Messenger

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 12640137

VL - 23

SP - 2564

EP - 2576

JO - MOL CELL BIOL

JF - MOL CELL BIOL

SN - 0270-7306

IS - 7

ER -