Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas
Beteiligte Einrichtungen
Abstract
Exocrine and endocrine pancreas are interconnected anatomically and functionally, with vasculature facilitating bidirectional communication. Our understanding of this network remains limited, largely due to two-dimensional histology and missing combination with three-dimensional imaging. In this study, a multiscale 3D-imaging process was used to analyze a porcine pancreas. Clinical computed tomography, digital volume tomography, micro-computed tomography and Synchrotron-based propagation-based imaging were applied consecutively. Fields of view correlated inversely with attainable resolution from a whole organism level down to capillary structures with a voxel edge length of 2.0 µm. Segmented vascular networks from 3D-imaging data were correlated with tissue sections stained by immunohistochemistry and revealed highly vascularized regions to be intra-islet capillaries of islets of Langerhans. Generated 3D-datasets allowed for three-dimensional qualitative and quantitative organ and vessel structure analysis. Beyond this study, the method shows potential for application across a wide range of patho-morphology analyses and might possibly provide microstructural blueprints for biotissue engineering.
Bibliografische Daten
Originalsprache | Englisch |
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ISSN | 2045-2322 |
DOIs | |
Status | Veröffentlicht - 02.05.2024 |
Anmerkungen des Dekanats
© 2024. The Author(s).
PubMed | 38698049 |
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