Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix
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Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix. / Stachnik, Karolina; Warmer, Martin ; Mohacsi, Istvan ; Hennicke, Vincent ; Fischer, Pontus ; Meyer, Jan ; Spitzbart, Tobias ; Barthelmess, Miriam ; Eich, Jacqueline; David, Christian ; Feldmann, Claus ; Busse, Björn; Jähn, Katharina; Schaible, Ulrich E. ; Meents, Alke .
in: SCI REP-UK, Jahrgang 10, Nr. 1, 04.02.2020, S. 1784.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix
AU - Stachnik, Karolina
AU - Warmer, Martin
AU - Mohacsi, Istvan
AU - Hennicke, Vincent
AU - Fischer, Pontus
AU - Meyer, Jan
AU - Spitzbart, Tobias
AU - Barthelmess, Miriam
AU - Eich, Jacqueline
AU - David, Christian
AU - Feldmann, Claus
AU - Busse, Björn
AU - Jähn, Katharina
AU - Schaible, Ulrich E.
AU - Meents, Alke
PY - 2020/2/4
Y1 - 2020/2/4
N2 - Studies of biological systems typically require the application of several complementary methods able to yield statistically-relevant results at a unique level of sensitivity. Combined X-ray fluorescence and ptychography offer excellent elemental and structural imaging contrasts at the nanoscale. They enable a robust correlation of elemental distributions with respect to the cellular morphology. Here we extend the applicability of the two modalities to higher X-ray excitation energies, permitting iron mapping. Using a long-range scanning setup, we applied the method to two vital biomedical cases. We quantified the iron distributions in a population of macrophages treated with Mycobacterium-tuberculosis-targeting iron-oxide nanocontainers. Our work allowed to visualize the internalization of the nanocontainer agglomerates in the cytosol. From the iron areal mass maps, we obtained a distribution of antibiotic load per agglomerate and an average areal concentration of nanocontainers in the agglomerates. In the second application we mapped the calcium content in a human bone matrix in close proximity to osteocyte lacunae (perilacunar matrix). A concurrently acquired ptychographic image was used to remove the mass-thickness effect from the raw calcium map. The resulting ptychography-enhanced calcium distribution allowed then to observe a locally lower degree of mineralization of the perilacunar matrix.
AB - Studies of biological systems typically require the application of several complementary methods able to yield statistically-relevant results at a unique level of sensitivity. Combined X-ray fluorescence and ptychography offer excellent elemental and structural imaging contrasts at the nanoscale. They enable a robust correlation of elemental distributions with respect to the cellular morphology. Here we extend the applicability of the two modalities to higher X-ray excitation energies, permitting iron mapping. Using a long-range scanning setup, we applied the method to two vital biomedical cases. We quantified the iron distributions in a population of macrophages treated with Mycobacterium-tuberculosis-targeting iron-oxide nanocontainers. Our work allowed to visualize the internalization of the nanocontainer agglomerates in the cytosol. From the iron areal mass maps, we obtained a distribution of antibiotic load per agglomerate and an average areal concentration of nanocontainers in the agglomerates. In the second application we mapped the calcium content in a human bone matrix in close proximity to osteocyte lacunae (perilacunar matrix). A concurrently acquired ptychographic image was used to remove the mass-thickness effect from the raw calcium map. The resulting ptychography-enhanced calcium distribution allowed then to observe a locally lower degree of mineralization of the perilacunar matrix.
U2 - https://doi.org/10.1038/s41598-020-58318-7
DO - https://doi.org/10.1038/s41598-020-58318-7
M3 - SCORING: Journal article
VL - 10
SP - 1784
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
IS - 1
ER -