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Multicolor RGB Marking Allows Morphometric and Functional Analysis of Hippocampal Granule Neurons at the Single-Cell Level

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Multicolor RGB Marking Allows Morphometric and Functional Analysis of Hippocampal Granule Neurons at the Single-Cell Level. / Gomez-Nicola, Diego; Riecken, Kristoffer; Perry, V Hugh; Fehse, Boris.

in: HUM GENE THER, Jahrgang 26, Nr. 6, 20.04.2015, S. 332-333.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Gomez-Nicola, D, Riecken, K, Perry, VH & Fehse, B 2015, 'Multicolor RGB Marking Allows Morphometric and Functional Analysis of Hippocampal Granule Neurons at the Single-Cell Level', HUM GENE THER, Jg. 26, Nr. 6, S. 332-333. https://doi.org/10.1089/hum.2015.010

APA

Gomez-Nicola, D., Riecken, K., Perry, V. H., & Fehse, B. (2015). Multicolor RGB Marking Allows Morphometric and Functional Analysis of Hippocampal Granule Neurons at the Single-Cell Level. HUM GENE THER, 26(6), 332-333. https://doi.org/10.1089/hum.2015.010

Vancouver

Gomez-Nicola D, Riecken K, Perry VH, Fehse B. Multicolor RGB Marking Allows Morphometric and Functional Analysis of Hippocampal Granule Neurons at the Single-Cell Level. HUM GENE THER. 2015 Apr 20;26(6):332-333. https://doi.org/10.1089/hum.2015.010

Bibtex

@article{476c1f4dda504bb6afb9e204c880492a,
title = "Multicolor RGB Marking Allows Morphometric and Functional Analysis of Hippocampal Granule Neurons at the Single-Cell Level",
abstract = "Identifying and tracking cells in the brain is a challenging task, requiring advanced molecular biology techniques and precise anatomical information. We have applied the principle of RGB marking to the long-term marking and tracking of progenitor and mature cells in the adult brain. Briefly, multicolor RGB marking is based on the simultaneous, lentiviral vector-mediated expression of three genes encoding fluorescent proteins in the three basic colors (red, green, and blue). Here, we show the application of RGB marking to the stable multicolor marking of adult granule cells in the hippocampus. This technique provides each individual cell with a characteristic hue, which facilitates their anatomical identification and spatial tracking. Multicolor RBG marking of mature neurons can provide an effective approach to dissect the function of individual cells, as it allows combination with functional techniques, facilitating the understanding of complex neuronal populations such as that of the dentate gyrus.",
author = "Diego Gomez-Nicola and Kristoffer Riecken and Perry, {V Hugh} and Boris Fehse",
year = "2015",
month = apr,
day = "20",
doi = "10.1089/hum.2015.010",
language = "English",
volume = "26",
pages = "332--333",
journal = "HUM GENE THER",
issn = "1043-0342",
publisher = "Mary Ann Liebert Inc.",
number = "6",

}

RIS

TY - JOUR

T1 - Multicolor RGB Marking Allows Morphometric and Functional Analysis of Hippocampal Granule Neurons at the Single-Cell Level

AU - Gomez-Nicola, Diego

AU - Riecken, Kristoffer

AU - Perry, V Hugh

AU - Fehse, Boris

PY - 2015/4/20

Y1 - 2015/4/20

N2 - Identifying and tracking cells in the brain is a challenging task, requiring advanced molecular biology techniques and precise anatomical information. We have applied the principle of RGB marking to the long-term marking and tracking of progenitor and mature cells in the adult brain. Briefly, multicolor RGB marking is based on the simultaneous, lentiviral vector-mediated expression of three genes encoding fluorescent proteins in the three basic colors (red, green, and blue). Here, we show the application of RGB marking to the stable multicolor marking of adult granule cells in the hippocampus. This technique provides each individual cell with a characteristic hue, which facilitates their anatomical identification and spatial tracking. Multicolor RBG marking of mature neurons can provide an effective approach to dissect the function of individual cells, as it allows combination with functional techniques, facilitating the understanding of complex neuronal populations such as that of the dentate gyrus.

AB - Identifying and tracking cells in the brain is a challenging task, requiring advanced molecular biology techniques and precise anatomical information. We have applied the principle of RGB marking to the long-term marking and tracking of progenitor and mature cells in the adult brain. Briefly, multicolor RGB marking is based on the simultaneous, lentiviral vector-mediated expression of three genes encoding fluorescent proteins in the three basic colors (red, green, and blue). Here, we show the application of RGB marking to the stable multicolor marking of adult granule cells in the hippocampus. This technique provides each individual cell with a characteristic hue, which facilitates their anatomical identification and spatial tracking. Multicolor RBG marking of mature neurons can provide an effective approach to dissect the function of individual cells, as it allows combination with functional techniques, facilitating the understanding of complex neuronal populations such as that of the dentate gyrus.

U2 - 10.1089/hum.2015.010

DO - 10.1089/hum.2015.010

M3 - SCORING: Journal article

C2 - 25894663

VL - 26

SP - 332

EP - 333

JO - HUM GENE THER

JF - HUM GENE THER

SN - 1043-0342

IS - 6

ER -