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Multicenter assessment of the rapid Unyvero Blood Culture molecular assay

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Multicenter assessment of the rapid Unyvero Blood Culture molecular assay. / Burrack-Lange, Sandra Christina; Personne, Yoann; Huber, Monika; Winkler, Elisabeth; Weile, Jan; Knabbe, Cornelius; Görig, Julia; Rohde, Holger.

in: J MED MICROBIOL, Jahrgang 67, Nr. 9, 09.2018, S. 1294-1301.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Burrack-Lange, SC, Personne, Y, Huber, M, Winkler, E, Weile, J, Knabbe, C, Görig, J & Rohde, H 2018, 'Multicenter assessment of the rapid Unyvero Blood Culture molecular assay', J MED MICROBIOL, Jg. 67, Nr. 9, S. 1294-1301. https://doi.org/10.1099/jmm.0.000804

APA

Burrack-Lange, S. C., Personne, Y., Huber, M., Winkler, E., Weile, J., Knabbe, C., Görig, J., & Rohde, H. (2018). Multicenter assessment of the rapid Unyvero Blood Culture molecular assay. J MED MICROBIOL, 67(9), 1294-1301. https://doi.org/10.1099/jmm.0.000804

Vancouver

Burrack-Lange SC, Personne Y, Huber M, Winkler E, Weile J, Knabbe C et al. Multicenter assessment of the rapid Unyvero Blood Culture molecular assay. J MED MICROBIOL. 2018 Sep;67(9):1294-1301. https://doi.org/10.1099/jmm.0.000804

Bibtex

@article{1242e738235d427093704f3021346ac2,
title = "Multicenter assessment of the rapid Unyvero Blood Culture molecular assay",
abstract = "PURPOSE: Bloodstream infections remain an important cause of morbidity and mortality. Rapid diagnosis can reduce the time from empiric antimicrobial therapy to targeted therapy and improve patient outcomes.METHODOLOGY: The fully automated Unyvero Blood Culture (BCU) Application (Curetis GmbH) can identify a broad panel of pathogens (36 analytes covering over 50 pathogens) and 16 antibiotic resistance gene markers simultaneously in about 5 h. The assay was evaluated in three clinical laboratories in comparison to routine microbiological procedures.RESULTS: A total of 207 blood cultures were included in the study, and 90.5 % of the species identified by culture were covered by the Unyvero BCU panel with an overall sensitivity of 96.8 % and specificity of 99.8 %. The time to result was reduced on average by about 34 h. The assay accurately identified 95 % of the species, including 158/164 monomicrobial and 7/9 polymicrobial cultures. The Unyvero BCU Cartridge detected a large number of resistance markers including mecA (n=57), aac(6')aph(2'') (n=40), one vanB resistance gene, and six instances of blaCTX-M.CONCLUSION: The Unyvero BCU Application provided fast, reliable results, while significantly improving turnaround time in blood culture diagnostics.",
keywords = "Anti-Bacterial Agents, Bacteremia, Bacteria, Bacterial Proteins, Blood Culture, Humans, Multiplex Polymerase Chain Reaction, Polymerase Chain Reaction, Sensitivity and Specificity, Evaluation Studies, Journal Article, Multicenter Study",
author = "Burrack-Lange, {Sandra Christina} and Yoann Personne and Monika Huber and Elisabeth Winkler and Jan Weile and Cornelius Knabbe and Julia G{\"o}rig and Holger Rohde",
year = "2018",
month = sep,
doi = "10.1099/jmm.0.000804",
language = "English",
volume = "67",
pages = "1294--1301",
journal = "J MED MICROBIOL",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "9",

}

RIS

TY - JOUR

T1 - Multicenter assessment of the rapid Unyvero Blood Culture molecular assay

AU - Burrack-Lange, Sandra Christina

AU - Personne, Yoann

AU - Huber, Monika

AU - Winkler, Elisabeth

AU - Weile, Jan

AU - Knabbe, Cornelius

AU - Görig, Julia

AU - Rohde, Holger

PY - 2018/9

Y1 - 2018/9

N2 - PURPOSE: Bloodstream infections remain an important cause of morbidity and mortality. Rapid diagnosis can reduce the time from empiric antimicrobial therapy to targeted therapy and improve patient outcomes.METHODOLOGY: The fully automated Unyvero Blood Culture (BCU) Application (Curetis GmbH) can identify a broad panel of pathogens (36 analytes covering over 50 pathogens) and 16 antibiotic resistance gene markers simultaneously in about 5 h. The assay was evaluated in three clinical laboratories in comparison to routine microbiological procedures.RESULTS: A total of 207 blood cultures were included in the study, and 90.5 % of the species identified by culture were covered by the Unyvero BCU panel with an overall sensitivity of 96.8 % and specificity of 99.8 %. The time to result was reduced on average by about 34 h. The assay accurately identified 95 % of the species, including 158/164 monomicrobial and 7/9 polymicrobial cultures. The Unyvero BCU Cartridge detected a large number of resistance markers including mecA (n=57), aac(6')aph(2'') (n=40), one vanB resistance gene, and six instances of blaCTX-M.CONCLUSION: The Unyvero BCU Application provided fast, reliable results, while significantly improving turnaround time in blood culture diagnostics.

AB - PURPOSE: Bloodstream infections remain an important cause of morbidity and mortality. Rapid diagnosis can reduce the time from empiric antimicrobial therapy to targeted therapy and improve patient outcomes.METHODOLOGY: The fully automated Unyvero Blood Culture (BCU) Application (Curetis GmbH) can identify a broad panel of pathogens (36 analytes covering over 50 pathogens) and 16 antibiotic resistance gene markers simultaneously in about 5 h. The assay was evaluated in three clinical laboratories in comparison to routine microbiological procedures.RESULTS: A total of 207 blood cultures were included in the study, and 90.5 % of the species identified by culture were covered by the Unyvero BCU panel with an overall sensitivity of 96.8 % and specificity of 99.8 %. The time to result was reduced on average by about 34 h. The assay accurately identified 95 % of the species, including 158/164 monomicrobial and 7/9 polymicrobial cultures. The Unyvero BCU Cartridge detected a large number of resistance markers including mecA (n=57), aac(6')aph(2'') (n=40), one vanB resistance gene, and six instances of blaCTX-M.CONCLUSION: The Unyvero BCU Application provided fast, reliable results, while significantly improving turnaround time in blood culture diagnostics.

KW - Anti-Bacterial Agents

KW - Bacteremia

KW - Bacteria

KW - Bacterial Proteins

KW - Blood Culture

KW - Humans

KW - Multiplex Polymerase Chain Reaction

KW - Polymerase Chain Reaction

KW - Sensitivity and Specificity

KW - Evaluation Studies

KW - Journal Article

KW - Multicenter Study

U2 - 10.1099/jmm.0.000804

DO - 10.1099/jmm.0.000804

M3 - SCORING: Journal article

C2 - 30051799

VL - 67

SP - 1294

EP - 1301

JO - J MED MICROBIOL

JF - J MED MICROBIOL

SN - 0022-2615

IS - 9

ER -