Morphologic analysis of three-dimensional tumors developed in fibrin matrix and agar culture system.
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Morphologic analysis of three-dimensional tumors developed in fibrin matrix and agar culture system. / Kumazawa, H; Hess, Markus; Koldovsky, P.
in: PATHOBIOLOGY, Jahrgang 61, Nr. 1, 1, 1993, S. 31-35.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Morphologic analysis of three-dimensional tumors developed in fibrin matrix and agar culture system.
AU - Kumazawa, H
AU - Hess, Markus
AU - Koldovsky, P
PY - 1993
Y1 - 1993
N2 - The three-dimensional growth of cultured tumor cell lines (HT29, a colon adenocarcinoma cell line; M21, a melanoma cell line; KB, a nasopharyngeal carcinoma cell line) has been investigated in an agar culture system with a fibrin matrix in vitro. The tumor cells developed to tumors 3 x 3 mm in diameter after 10 days in culture in vitro. This size was large enough to allow histologic examination. The tumor cells located in the surface area of the three-dimensional tumor seemed to grow well. However, the tumor cells in the center degenerated or did not proliferate, indicating a lack of nutrition and/or anoxia in the center. The histologic comparison between the xenografted tumors on nude mice and the three-dimensional tumors in vitro suggests that the structures of the three-dimensional tumors were comparable, especially in the surface area, to the xenografted tumors. Furthermore, the antitumor effect of mitomycin C on the three-dimensional tumors was found to be dose-dependent.
AB - The three-dimensional growth of cultured tumor cell lines (HT29, a colon adenocarcinoma cell line; M21, a melanoma cell line; KB, a nasopharyngeal carcinoma cell line) has been investigated in an agar culture system with a fibrin matrix in vitro. The tumor cells developed to tumors 3 x 3 mm in diameter after 10 days in culture in vitro. This size was large enough to allow histologic examination. The tumor cells located in the surface area of the three-dimensional tumor seemed to grow well. However, the tumor cells in the center degenerated or did not proliferate, indicating a lack of nutrition and/or anoxia in the center. The histologic comparison between the xenografted tumors on nude mice and the three-dimensional tumors in vitro suggests that the structures of the three-dimensional tumors were comparable, especially in the surface area, to the xenografted tumors. Furthermore, the antitumor effect of mitomycin C on the three-dimensional tumors was found to be dose-dependent.
M3 - SCORING: Zeitschriftenaufsatz
VL - 61
SP - 31
EP - 35
JO - PATHOBIOLOGY
JF - PATHOBIOLOGY
SN - 1015-2008
IS - 1
M1 - 1
ER -
