Monoclonal antibody to a supertypic determinant associated with HLA-DRw52.
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Monoclonal antibody to a supertypic determinant associated with HLA-DRw52. / Eiermann, Thomas; Krause, S; Wölpl, A; Ballas, M; Goldmann, S F.
in: HYBRIDOMA, Jahrgang 8, Nr. 4, 4, 1989, S. 467-474.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Monoclonal antibody to a supertypic determinant associated with HLA-DRw52.
AU - Eiermann, Thomas
AU - Krause, S
AU - Wölpl, A
AU - Ballas, M
AU - Goldmann, S F
PY - 1989
Y1 - 1989
N2 - A cytotoxic mouse monoclonal antibody UL-52 (IgG2b) was obtained after immunization of a BALB/c mouse with the lymphoblastoid cell line STA homozygous for HLA-A3, B8, Cw7, DR3, DRw52, DQw2, DPw2. Fluorescence analysis on a panel of B lymphoblastoid cell lines from the 10th International Histocompatibility Workshop 1987 showed almost exact concordance of UL-52 reactivity with the presence of the HLA-DRw52 antigen. Cytotoxicity testing of UL-52 on mononuclear cells of HLA-typed individuals revealed a pattern of reactivity closely associated with the HLA-DRw52 specificity as defined by conventional alloantisera (R = 0.77). UL-52 precipitated appropriate 29,000- and 33,000-dalton bands on SDS- polyacrylamide gels under reducing conditions from an HLA-DRw52 positive B lymphoblastoid cell line. Thus, by serological and biochemical criteria UL-52 defines a supertypic determinant associated with HLA-DRw52. In contrast to most DRw52- like monoclonal antibodies, UL-52 binds to DRw8 positive cells.
AB - A cytotoxic mouse monoclonal antibody UL-52 (IgG2b) was obtained after immunization of a BALB/c mouse with the lymphoblastoid cell line STA homozygous for HLA-A3, B8, Cw7, DR3, DRw52, DQw2, DPw2. Fluorescence analysis on a panel of B lymphoblastoid cell lines from the 10th International Histocompatibility Workshop 1987 showed almost exact concordance of UL-52 reactivity with the presence of the HLA-DRw52 antigen. Cytotoxicity testing of UL-52 on mononuclear cells of HLA-typed individuals revealed a pattern of reactivity closely associated with the HLA-DRw52 specificity as defined by conventional alloantisera (R = 0.77). UL-52 precipitated appropriate 29,000- and 33,000-dalton bands on SDS- polyacrylamide gels under reducing conditions from an HLA-DRw52 positive B lymphoblastoid cell line. Thus, by serological and biochemical criteria UL-52 defines a supertypic determinant associated with HLA-DRw52. In contrast to most DRw52- like monoclonal antibodies, UL-52 binds to DRw8 positive cells.
M3 - SCORING: Zeitschriftenaufsatz
VL - 8
SP - 467
EP - 474
IS - 4
M1 - 4
ER -
