Molecular characterization and in situ mRNA localization of the neural recognition molecule J1-160/180
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Molecular characterization and in situ mRNA localization of the neural recognition molecule J1-160/180 : a modular structure similar to tenascin. / Fuss, B; Wintergerst, E S; Bartsch, U; Schachner, M.
in: J CELL BIOL, Jahrgang 120, Nr. 5, 03.1993, S. 1237-49.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Molecular characterization and in situ mRNA localization of the neural recognition molecule J1-160/180
T2 - a modular structure similar to tenascin
AU - Fuss, B
AU - Wintergerst, E S
AU - Bartsch, U
AU - Schachner, M
PY - 1993/3
Y1 - 1993/3
N2 - The oligodendrocyte-derived extracellular matrix glycoprotein J1-160/180 is a recognition molecule expressed exclusively in the central nervous system. J1-160/180 has been shown to be adhesive for astrocytes and repellent towards neurons and growth cones. We report here the complete nucleotide sequence of J1-160/180 in the rat. The predicted amino acid sequence showed a structural architecture very similar to tenascin: a cysteine-rich amino terminal region is followed by 4.5 epidermal growth factor-like repeats, 9 fibronectin type III homologous repeats and a domain homologous to fibrinogen. Sequence comparison analysis revealed highest homology of rat J1-160/180 to mouse tenascin and chicken restrictin with a similarity of 66% and 85%, respectively. The J1-160/180-coding mRNA is derived from a single copy gene. Using the polymerase chain reaction we could show that two J1-160/180 isoforms are generated by alternative splicing of the sixth fibronectin type III homologous repeat. Localization of J1-160/180 mRNA by in situ hybridization in the cerebellum, hippocampus and olfactory bulb confirmed the expression of J1-160/180 by oligodendrocytes with a peak of transcription at 7-14 d after birth, indicating a functional role during myelination. In addition, J1-160/180-specific RNA was found in a small subset of neurons in all three structures of the CNS analyzed. These neurons continue to express J1-160/180 in the adult.
AB - The oligodendrocyte-derived extracellular matrix glycoprotein J1-160/180 is a recognition molecule expressed exclusively in the central nervous system. J1-160/180 has been shown to be adhesive for astrocytes and repellent towards neurons and growth cones. We report here the complete nucleotide sequence of J1-160/180 in the rat. The predicted amino acid sequence showed a structural architecture very similar to tenascin: a cysteine-rich amino terminal region is followed by 4.5 epidermal growth factor-like repeats, 9 fibronectin type III homologous repeats and a domain homologous to fibrinogen. Sequence comparison analysis revealed highest homology of rat J1-160/180 to mouse tenascin and chicken restrictin with a similarity of 66% and 85%, respectively. The J1-160/180-coding mRNA is derived from a single copy gene. Using the polymerase chain reaction we could show that two J1-160/180 isoforms are generated by alternative splicing of the sixth fibronectin type III homologous repeat. Localization of J1-160/180 mRNA by in situ hybridization in the cerebellum, hippocampus and olfactory bulb confirmed the expression of J1-160/180 by oligodendrocytes with a peak of transcription at 7-14 d after birth, indicating a functional role during myelination. In addition, J1-160/180-specific RNA was found in a small subset of neurons in all three structures of the CNS analyzed. These neurons continue to express J1-160/180 in the adult.
KW - Alternative Splicing
KW - Animals
KW - Base Sequence
KW - Cell Adhesion
KW - Cell Adhesion Molecules, Neuronal
KW - Cloning, Molecular
KW - DNA
KW - Extracellular Matrix Proteins
KW - In Situ Hybridization
KW - Molecular Sequence Data
KW - Nerve Tissue Proteins
KW - Oligodeoxyribonucleotides
KW - Polymerase Chain Reaction
KW - RNA, Messenger
KW - Rats
KW - Tenascin
KW - Tissue Distribution
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - SCORING: Journal article
C2 - 7679676
VL - 120
SP - 1237
EP - 1249
JO - J CELL BIOL
JF - J CELL BIOL
SN - 0021-9525
IS - 5
ER -