PortalPublikationen"Molecular Activity Painting"

"Molecular Activity Painting"

Standard

"Molecular Activity Painting" : Switch-like, Light-Controlled Perturbations inside Living Cells. / Chen, Xi; Venkatachalapathy, Muthukumaran; Kamps, Dominic; Weigel, Simone; Kumar, Ravi; Orlich, Michael; Garrecht, Ruben; Hirtz, Michael; Niemeyer, Christof M; Wu, Yao-Wen; Dehmelt, Leif.

in: ANGEW CHEM INT EDIT, Jahrgang 56, Nr. 21, 15.05.2017, S. 5916-5920.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Chen, X, Venkatachalapathy, M, Kamps, D, Weigel, S, Kumar, R, Orlich, M, Garrecht, R, Hirtz, M, Niemeyer, CM, Wu, Y-W & Dehmelt, L 2017, '"Molecular Activity Painting": Switch-like, Light-Controlled Perturbations inside Living Cells', ANGEW CHEM INT EDIT, Jg. 56, Nr. 21, S. 5916-5920. https://doi.org/10.1002/anie.201611432

APA

Chen, X., Venkatachalapathy, M., Kamps, D., Weigel, S., Kumar, R., Orlich, M., Garrecht, R., Hirtz, M., Niemeyer, C. M., Wu, Y-W., & Dehmelt, L. (2017). "Molecular Activity Painting": Switch-like, Light-Controlled Perturbations inside Living Cells. ANGEW CHEM INT EDIT, 56(21), 5916-5920. https://doi.org/10.1002/anie.201611432

Vancouver

Chen X, Venkatachalapathy M, Kamps D, Weigel S, Kumar R, Orlich M et al. "Molecular Activity Painting": Switch-like, Light-Controlled Perturbations inside Living Cells. ANGEW CHEM INT EDIT. 2017 Mai 15;56(21):5916-5920. https://doi.org/10.1002/anie.201611432

Bibtex

@article{7e44b728f974418f8a5b6946b2cdcce8,
title = "{"}Molecular Activity Painting{"}: Switch-like, Light-Controlled Perturbations inside Living Cells",
abstract = "Acute subcellular protein targeting is a powerful tool to study biological networks. However, signaling at the plasma membrane is highly dynamic, making it difficult to study in space and time. In particular, sustained local control of molecular function is challenging owing to the lateral diffusion of plasma membrane targeted molecules. Herein we present {"}molecular activity painting{"} (MAP), a novel technology which combines photoactivatable chemically induced dimerization (pCID) with immobilized artificial receptors. The immobilization of artificial receptors by surface-immobilized antibodies blocks lateral diffusion, enabling rapid and stable {"}painting{"} of signaling molecules and their activity at the plasma membrane with micrometer precision. Using this method, we show that painting of the RhoA-myosin activator GEF-H1 induces patterned acto-myosin contraction inside living cells.",
keywords = "Cell Membrane/chemistry, Cells, Cultured, DNA-Binding Proteins/chemistry, Dimerization, Inventions/trends, Light, Transcription Factors/chemistry",
author = "Xi Chen and Muthukumaran Venkatachalapathy and Dominic Kamps and Simone Weigel and Ravi Kumar and Michael Orlich and Ruben Garrecht and Michael Hirtz and Niemeyer, {Christof M} and Yao-Wen Wu and Leif Dehmelt",
note = "{\textcopyright} 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2017",
month = may,
day = "15",
doi = "10.1002/anie.201611432",
language = "English",
volume = "56",
pages = "5916--5920",
journal = "ANGEW CHEM INT EDIT",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "21",

}

RIS

TY - JOUR

T1 - "Molecular Activity Painting"

T2 - Switch-like, Light-Controlled Perturbations inside Living Cells

AU - Chen, Xi

AU - Venkatachalapathy, Muthukumaran

AU - Kamps, Dominic

AU - Weigel, Simone

AU - Kumar, Ravi

AU - Orlich, Michael

AU - Garrecht, Ruben

AU - Hirtz, Michael

AU - Niemeyer, Christof M

AU - Wu, Yao-Wen

AU - Dehmelt, Leif

N1 - © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2017/5/15

Y1 - 2017/5/15

N2 - Acute subcellular protein targeting is a powerful tool to study biological networks. However, signaling at the plasma membrane is highly dynamic, making it difficult to study in space and time. In particular, sustained local control of molecular function is challenging owing to the lateral diffusion of plasma membrane targeted molecules. Herein we present "molecular activity painting" (MAP), a novel technology which combines photoactivatable chemically induced dimerization (pCID) with immobilized artificial receptors. The immobilization of artificial receptors by surface-immobilized antibodies blocks lateral diffusion, enabling rapid and stable "painting" of signaling molecules and their activity at the plasma membrane with micrometer precision. Using this method, we show that painting of the RhoA-myosin activator GEF-H1 induces patterned acto-myosin contraction inside living cells.

AB - Acute subcellular protein targeting is a powerful tool to study biological networks. However, signaling at the plasma membrane is highly dynamic, making it difficult to study in space and time. In particular, sustained local control of molecular function is challenging owing to the lateral diffusion of plasma membrane targeted molecules. Herein we present "molecular activity painting" (MAP), a novel technology which combines photoactivatable chemically induced dimerization (pCID) with immobilized artificial receptors. The immobilization of artificial receptors by surface-immobilized antibodies blocks lateral diffusion, enabling rapid and stable "painting" of signaling molecules and their activity at the plasma membrane with micrometer precision. Using this method, we show that painting of the RhoA-myosin activator GEF-H1 induces patterned acto-myosin contraction inside living cells.

KW - Cell Membrane/chemistry

KW - Cells, Cultured

KW - DNA-Binding Proteins/chemistry

KW - Dimerization

KW - Inventions/trends

KW - Light

KW - Transcription Factors/chemistry

U2 - 10.1002/anie.201611432

DO - 10.1002/anie.201611432

M3 - SCORING: Journal article

C2 - 28370940

VL - 56

SP - 5916

EP - 5920

JO - ANGEW CHEM INT EDIT

JF - ANGEW CHEM INT EDIT

SN - 1433-7851

IS - 21

ER -