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Modulation of arachidonic and linoleic acid metabolites in myeloperoxidase-deficient mice during acute inflammation

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Modulation of arachidonic and linoleic acid metabolites in myeloperoxidase-deficient mice during acute inflammation. / Kubala, Lukas; Schmelzer, Kara R; Klinke, Anna; Kolarova, Hana; Baldus, Stephan; Hammock, Bruce D; Eiserich, Jason P.

in: FREE RADICAL BIO MED, Jahrgang 48, Nr. 10, 15.05.2010, S. 1311-1320.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kubala, L, Schmelzer, KR, Klinke, A, Kolarova, H, Baldus, S, Hammock, BD & Eiserich, JP 2010, 'Modulation of arachidonic and linoleic acid metabolites in myeloperoxidase-deficient mice during acute inflammation', FREE RADICAL BIO MED, Jg. 48, Nr. 10, S. 1311-1320. https://doi.org/10.1016/j.freeradbiomed.2010.02.010

APA

Kubala, L., Schmelzer, K. R., Klinke, A., Kolarova, H., Baldus, S., Hammock, B. D., & Eiserich, J. P. (2010). Modulation of arachidonic and linoleic acid metabolites in myeloperoxidase-deficient mice during acute inflammation. FREE RADICAL BIO MED, 48(10), 1311-1320. https://doi.org/10.1016/j.freeradbiomed.2010.02.010

Vancouver

Kubala L, Schmelzer KR, Klinke A, Kolarova H, Baldus S, Hammock BD et al. Modulation of arachidonic and linoleic acid metabolites in myeloperoxidase-deficient mice during acute inflammation. FREE RADICAL BIO MED. 2010 Mai 15;48(10):1311-1320. https://doi.org/10.1016/j.freeradbiomed.2010.02.010

Bibtex

@article{2e8e3706f977467bbfb4780be85a7ad3,
title = "Modulation of arachidonic and linoleic acid metabolites in myeloperoxidase-deficient mice during acute inflammation",
abstract = "Acute inflammation is a common feature of many life-threatening pathologies, including septic shock. One hallmark of acute inflammation is the peroxidation of polyunsaturated fatty acids forming bioactive products that regulate inflammation. Myeloperoxidase (MPO) is an abundant phagocyte-derived hemoprotein released during phagocyte activation. Here, we investigated the role of MPO in modulating biologically active arachidonic acid (AA) and linoleic acid (LA) metabolites during acute inflammation. Wild-type and MPO-knockout (KO) mice were exposed to intraperitoneally injected endotoxin for 24 h, and plasma LA and AA oxidation products were comprehensively analyzed using a liquid chromatography-mass spectrometry method. Compared to wild-type mice, MPO-KO mice had significantly lower plasma levels of LA epoxides and corresponding LA- and AA-derived fatty acid diols. AA and LA hydroxy intermediates (hydroxyeicosatetraenoic and hydroxyoctadecadienoic acids) were also significantly lower in MPO-KO mice. Conversely, MPO-deficient mice had significantly higher plasma levels of cysteinyl-leukotrienes with well-known proinflammatory properties. In vitro experiments revealed significantly lower amounts of AA and LA epoxides, LA- and AA-derived fatty acid diols, and AA and LA hydroxy intermediates in stimulated polymorphonuclear neutrophils isolated from MPO-KO mice. Our results demonstrate that MPO modulates the balance of pro- and anti-inflammatory lipid mediators during acute inflammation and, in this way, may control acute inflammatory diseases.",
keywords = "Animals, Arachidonic Acid/metabolism, Chromatography, Liquid, Disease Models, Animal, Epoxy Compounds/blood, Fatty Acids, Unsaturated/blood, Hydroxyeicosatetraenoic Acids/blood, Inflammation, Linoleic Acid/metabolism, Lipopolysaccharides/administration & dosage, Male, Mass Spectrometry, Mice, Mice, Inbred C57BL, Mice, Knockout, Neutrophils/metabolism, Peroxidase/genetics, Shock, Septic/blood",
author = "Lukas Kubala and Schmelzer, {Kara R} and Anna Klinke and Hana Kolarova and Stephan Baldus and Hammock, {Bruce D} and Eiserich, {Jason P}",
note = "Copyright 2010 Elsevier Inc. All rights reserved.",
year = "2010",
month = may,
day = "15",
doi = "10.1016/j.freeradbiomed.2010.02.010",
language = "English",
volume = "48",
pages = "1311--1320",
journal = "FREE RADICAL BIO MED",
issn = "0891-5849",
publisher = "Elsevier Inc.",
number = "10",

}

RIS

TY - JOUR

T1 - Modulation of arachidonic and linoleic acid metabolites in myeloperoxidase-deficient mice during acute inflammation

AU - Kubala, Lukas

AU - Schmelzer, Kara R

AU - Klinke, Anna

AU - Kolarova, Hana

AU - Baldus, Stephan

AU - Hammock, Bruce D

AU - Eiserich, Jason P

N1 - Copyright 2010 Elsevier Inc. All rights reserved.

PY - 2010/5/15

Y1 - 2010/5/15

N2 - Acute inflammation is a common feature of many life-threatening pathologies, including septic shock. One hallmark of acute inflammation is the peroxidation of polyunsaturated fatty acids forming bioactive products that regulate inflammation. Myeloperoxidase (MPO) is an abundant phagocyte-derived hemoprotein released during phagocyte activation. Here, we investigated the role of MPO in modulating biologically active arachidonic acid (AA) and linoleic acid (LA) metabolites during acute inflammation. Wild-type and MPO-knockout (KO) mice were exposed to intraperitoneally injected endotoxin for 24 h, and plasma LA and AA oxidation products were comprehensively analyzed using a liquid chromatography-mass spectrometry method. Compared to wild-type mice, MPO-KO mice had significantly lower plasma levels of LA epoxides and corresponding LA- and AA-derived fatty acid diols. AA and LA hydroxy intermediates (hydroxyeicosatetraenoic and hydroxyoctadecadienoic acids) were also significantly lower in MPO-KO mice. Conversely, MPO-deficient mice had significantly higher plasma levels of cysteinyl-leukotrienes with well-known proinflammatory properties. In vitro experiments revealed significantly lower amounts of AA and LA epoxides, LA- and AA-derived fatty acid diols, and AA and LA hydroxy intermediates in stimulated polymorphonuclear neutrophils isolated from MPO-KO mice. Our results demonstrate that MPO modulates the balance of pro- and anti-inflammatory lipid mediators during acute inflammation and, in this way, may control acute inflammatory diseases.

AB - Acute inflammation is a common feature of many life-threatening pathologies, including septic shock. One hallmark of acute inflammation is the peroxidation of polyunsaturated fatty acids forming bioactive products that regulate inflammation. Myeloperoxidase (MPO) is an abundant phagocyte-derived hemoprotein released during phagocyte activation. Here, we investigated the role of MPO in modulating biologically active arachidonic acid (AA) and linoleic acid (LA) metabolites during acute inflammation. Wild-type and MPO-knockout (KO) mice were exposed to intraperitoneally injected endotoxin for 24 h, and plasma LA and AA oxidation products were comprehensively analyzed using a liquid chromatography-mass spectrometry method. Compared to wild-type mice, MPO-KO mice had significantly lower plasma levels of LA epoxides and corresponding LA- and AA-derived fatty acid diols. AA and LA hydroxy intermediates (hydroxyeicosatetraenoic and hydroxyoctadecadienoic acids) were also significantly lower in MPO-KO mice. Conversely, MPO-deficient mice had significantly higher plasma levels of cysteinyl-leukotrienes with well-known proinflammatory properties. In vitro experiments revealed significantly lower amounts of AA and LA epoxides, LA- and AA-derived fatty acid diols, and AA and LA hydroxy intermediates in stimulated polymorphonuclear neutrophils isolated from MPO-KO mice. Our results demonstrate that MPO modulates the balance of pro- and anti-inflammatory lipid mediators during acute inflammation and, in this way, may control acute inflammatory diseases.

KW - Animals

KW - Arachidonic Acid/metabolism

KW - Chromatography, Liquid

KW - Disease Models, Animal

KW - Epoxy Compounds/blood

KW - Fatty Acids, Unsaturated/blood

KW - Hydroxyeicosatetraenoic Acids/blood

KW - Inflammation

KW - Linoleic Acid/metabolism

KW - Lipopolysaccharides/administration & dosage

KW - Male

KW - Mass Spectrometry

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Neutrophils/metabolism

KW - Peroxidase/genetics

KW - Shock, Septic/blood

U2 - 10.1016/j.freeradbiomed.2010.02.010

DO - 10.1016/j.freeradbiomed.2010.02.010

M3 - SCORING: Journal article

C2 - 20156554

VL - 48

SP - 1311

EP - 1320

JO - FREE RADICAL BIO MED

JF - FREE RADICAL BIO MED

SN - 0891-5849

IS - 10

ER -