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miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction

Standard

miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction. / Saul, Meike J; Baumann, Isabell; Bruno, Annalisa; Emmerich, Anne C; Wellstein, Julia; Ottinger, Sarah M; Contursi, Annalisa; Dovizio, Melania; Donnini, Sandra; Tacconelli, Stefania; Raouf, Joan; Idborg, Helena; Stein, Stefan; Korotkova, Marina; Savai, Rajkumar; Terzuoli, Erika; Sala, Gianluca; Seeger, Werner; Jakobsson, Per-Johan; Patrignani, Paola; Suess, Beatrix; Steinhilber, Dieter.

in: FASEB J, Jahrgang 33, Nr. 6, 06.2019, S. 6933-6947.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Saul, MJ, Baumann, I, Bruno, A, Emmerich, AC, Wellstein, J, Ottinger, SM, Contursi, A, Dovizio, M, Donnini, S, Tacconelli, S, Raouf, J, Idborg, H, Stein, S, Korotkova, M, Savai, R, Terzuoli, E, Sala, G, Seeger, W, Jakobsson, P-J, Patrignani, P, Suess, B & Steinhilber, D 2019, 'miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction', FASEB J, Jg. 33, Nr. 6, S. 6933-6947. https://doi.org/10.1096/fj.201802547R

APA

Saul, M. J., Baumann, I., Bruno, A., Emmerich, A. C., Wellstein, J., Ottinger, S. M., Contursi, A., Dovizio, M., Donnini, S., Tacconelli, S., Raouf, J., Idborg, H., Stein, S., Korotkova, M., Savai, R., Terzuoli, E., Sala, G., Seeger, W., Jakobsson, P-J., ... Steinhilber, D. (2019). miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction. FASEB J, 33(6), 6933-6947. https://doi.org/10.1096/fj.201802547R

Vancouver

Saul MJ, Baumann I, Bruno A, Emmerich AC, Wellstein J, Ottinger SM et al. miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction. FASEB J. 2019 Jun;33(6):6933-6947. https://doi.org/10.1096/fj.201802547R

Bibtex

@article{3e3297fc83d549bb95caeda75719a720,
title = "miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction",
abstract = "MicroRNAs (miRs) are important posttranscriptional regulators of gene expression. Besides their well-characterized inhibitory effects on mRNA stability and translation, miRs can also activate gene expression. In this study, we identified a novel noncanonical function of miR-574-5p. We found that miR-574-5p acts as an RNA decoy to CUG RNA-binding protein 1 (CUGBP1) and antagonizes its function. MiR-574-5p induces microsomal prostaglandin E synthase-1 (mPGES-1) expression by preventing CUGBP1 binding to its 3'UTR, leading to an enhanced alternative splicing and generation of an mPGES-1 3'UTR isoform, increased mPGES-1 protein expression, PGE2 formation, and tumor growth in vivo. miR-574-5p-induced tumor growth in mice could be completely inhibited with the mPGES-1 inhibitor CIII. Moreover, miR-574-5p is induced by IL-1β and is strongly overexpressed in human nonsmall cell lung cancer where high mPGES-1 expression correlates with a low survival rate. The discovered function of miR-574-5p as a CUGBP1 decoy opens up new therapeutic opportunities. It might serve as a stratification marker to select lung tumor patients who respond to the pharmacological inhibition of PGE2 formation.-Saul, M. J., Baumann, I., Bruno, A., Emmerich, A. C., Wellstein, J., Ottinger, S. M., Contursi, A., Dovizio, M., Donnini, S., Tacconelli, S., Raouf, J., Idborg, H., Stein, S., Korotkova, M., Savai, R., Terzuoli, E., Sala, G., Seeger, W., Jakobsson, P.-J., Patrignani, P., Suess, B., Steinhilber, D. miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction.",
keywords = "A549 Cells, Animals, CELF1 Protein/genetics, Cell Proliferation, Gene Expression Regulation, Neoplastic/drug effects, HeLa Cells, Humans, Mice, Mice, Nude, MicroRNAs/genetics, Molecular Mimicry, Neoplasms, Experimental, Prostaglandin-E Synthases/genetics, Protein Binding, Protein Synthesis Inhibitors/pharmacology, Puromycin/pharmacology, RNA/genetics, RNA Interference, RNA Isoforms, RNA, Messenger",
author = "Saul, {Meike J} and Isabell Baumann and Annalisa Bruno and Emmerich, {Anne C} and Julia Wellstein and Ottinger, {Sarah M} and Annalisa Contursi and Melania Dovizio and Sandra Donnini and Stefania Tacconelli and Joan Raouf and Helena Idborg and Stefan Stein and Marina Korotkova and Rajkumar Savai and Erika Terzuoli and Gianluca Sala and Werner Seeger and Per-Johan Jakobsson and Paola Patrignani and Beatrix Suess and Dieter Steinhilber",
year = "2019",
month = jun,
doi = "10.1096/fj.201802547R",
language = "English",
volume = "33",
pages = "6933--6947",
journal = "FASEB J",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

RIS

TY - JOUR

T1 - miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction

AU - Saul, Meike J

AU - Baumann, Isabell

AU - Bruno, Annalisa

AU - Emmerich, Anne C

AU - Wellstein, Julia

AU - Ottinger, Sarah M

AU - Contursi, Annalisa

AU - Dovizio, Melania

AU - Donnini, Sandra

AU - Tacconelli, Stefania

AU - Raouf, Joan

AU - Idborg, Helena

AU - Stein, Stefan

AU - Korotkova, Marina

AU - Savai, Rajkumar

AU - Terzuoli, Erika

AU - Sala, Gianluca

AU - Seeger, Werner

AU - Jakobsson, Per-Johan

AU - Patrignani, Paola

AU - Suess, Beatrix

AU - Steinhilber, Dieter

PY - 2019/6

Y1 - 2019/6

N2 - MicroRNAs (miRs) are important posttranscriptional regulators of gene expression. Besides their well-characterized inhibitory effects on mRNA stability and translation, miRs can also activate gene expression. In this study, we identified a novel noncanonical function of miR-574-5p. We found that miR-574-5p acts as an RNA decoy to CUG RNA-binding protein 1 (CUGBP1) and antagonizes its function. MiR-574-5p induces microsomal prostaglandin E synthase-1 (mPGES-1) expression by preventing CUGBP1 binding to its 3'UTR, leading to an enhanced alternative splicing and generation of an mPGES-1 3'UTR isoform, increased mPGES-1 protein expression, PGE2 formation, and tumor growth in vivo. miR-574-5p-induced tumor growth in mice could be completely inhibited with the mPGES-1 inhibitor CIII. Moreover, miR-574-5p is induced by IL-1β and is strongly overexpressed in human nonsmall cell lung cancer where high mPGES-1 expression correlates with a low survival rate. The discovered function of miR-574-5p as a CUGBP1 decoy opens up new therapeutic opportunities. It might serve as a stratification marker to select lung tumor patients who respond to the pharmacological inhibition of PGE2 formation.-Saul, M. J., Baumann, I., Bruno, A., Emmerich, A. C., Wellstein, J., Ottinger, S. M., Contursi, A., Dovizio, M., Donnini, S., Tacconelli, S., Raouf, J., Idborg, H., Stein, S., Korotkova, M., Savai, R., Terzuoli, E., Sala, G., Seeger, W., Jakobsson, P.-J., Patrignani, P., Suess, B., Steinhilber, D. miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction.

AB - MicroRNAs (miRs) are important posttranscriptional regulators of gene expression. Besides their well-characterized inhibitory effects on mRNA stability and translation, miRs can also activate gene expression. In this study, we identified a novel noncanonical function of miR-574-5p. We found that miR-574-5p acts as an RNA decoy to CUG RNA-binding protein 1 (CUGBP1) and antagonizes its function. MiR-574-5p induces microsomal prostaglandin E synthase-1 (mPGES-1) expression by preventing CUGBP1 binding to its 3'UTR, leading to an enhanced alternative splicing and generation of an mPGES-1 3'UTR isoform, increased mPGES-1 protein expression, PGE2 formation, and tumor growth in vivo. miR-574-5p-induced tumor growth in mice could be completely inhibited with the mPGES-1 inhibitor CIII. Moreover, miR-574-5p is induced by IL-1β and is strongly overexpressed in human nonsmall cell lung cancer where high mPGES-1 expression correlates with a low survival rate. The discovered function of miR-574-5p as a CUGBP1 decoy opens up new therapeutic opportunities. It might serve as a stratification marker to select lung tumor patients who respond to the pharmacological inhibition of PGE2 formation.-Saul, M. J., Baumann, I., Bruno, A., Emmerich, A. C., Wellstein, J., Ottinger, S. M., Contursi, A., Dovizio, M., Donnini, S., Tacconelli, S., Raouf, J., Idborg, H., Stein, S., Korotkova, M., Savai, R., Terzuoli, E., Sala, G., Seeger, W., Jakobsson, P.-J., Patrignani, P., Suess, B., Steinhilber, D. miR-574-5p as RNA decoy for CUGBP1 stimulates human lung tumor growth by mPGES-1 induction.

KW - A549 Cells

KW - Animals

KW - CELF1 Protein/genetics

KW - Cell Proliferation

KW - Gene Expression Regulation, Neoplastic/drug effects

KW - HeLa Cells

KW - Humans

KW - Mice

KW - Mice, Nude

KW - MicroRNAs/genetics

KW - Molecular Mimicry

KW - Neoplasms, Experimental

KW - Prostaglandin-E Synthases/genetics

KW - Protein Binding

KW - Protein Synthesis Inhibitors/pharmacology

KW - Puromycin/pharmacology

KW - RNA/genetics

KW - RNA Interference

KW - RNA Isoforms

KW - RNA, Messenger

U2 - 10.1096/fj.201802547R

DO - 10.1096/fj.201802547R

M3 - SCORING: Journal article

C2 - 30922080

VL - 33

SP - 6933

EP - 6947

JO - FASEB J

JF - FASEB J

SN - 0892-6638

IS - 6

ER -