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miR-148a is upregulated by Twist1 and T-bet and promotes Th1-cell survival by regulating the proapoptotic gene Bim

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miR-148a is upregulated by Twist1 and T-bet and promotes Th1-cell survival by regulating the proapoptotic gene Bim. / Haftmann, Claudia; Stittrich, Anna-Barbara; Zimmermann, Jakob; Fang, Zhuo; Hradilkova, Kristyna; Bardua, Markus; Westendorf, Kerstin; Heinz, Gitta A; Riedel, René; Siede, Julia; Lehmann, Katrin; Weinberger, Esther E; Zimmel, David; Lauer, Uta; Häupl, Thomas; Sieper, Joachim; Backhaus, Marina; Neumann, Christian; Hoffmann, Ute; Porstner, Martina; Chen, Wei; Grün, Joachim R; Baumgrass, Ria; Matz, Mareen; Löhning, Max; Scheffold, Alexander; Wittmann, Jürgen; Chang, Hyun-Dong; Rajewsky, Nikolaus; Jäck, Hans-Martin; Radbruch, Andreas; Mashreghi, Mir-Farzin.

in: EUR J IMMUNOL, Jahrgang 45, Nr. 4, 04.2015, S. 1192-205.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Haftmann, C, Stittrich, A-B, Zimmermann, J, Fang, Z, Hradilkova, K, Bardua, M, Westendorf, K, Heinz, GA, Riedel, R, Siede, J, Lehmann, K, Weinberger, EE, Zimmel, D, Lauer, U, Häupl, T, Sieper, J, Backhaus, M, Neumann, C, Hoffmann, U, Porstner, M, Chen, W, Grün, JR, Baumgrass, R, Matz, M, Löhning, M, Scheffold, A, Wittmann, J, Chang, H-D, Rajewsky, N, Jäck, H-M, Radbruch, A & Mashreghi, M-F 2015, 'miR-148a is upregulated by Twist1 and T-bet and promotes Th1-cell survival by regulating the proapoptotic gene Bim', EUR J IMMUNOL, Jg. 45, Nr. 4, S. 1192-205. https://doi.org/10.1002/eji.201444633

APA

Haftmann, C., Stittrich, A-B., Zimmermann, J., Fang, Z., Hradilkova, K., Bardua, M., Westendorf, K., Heinz, G. A., Riedel, R., Siede, J., Lehmann, K., Weinberger, E. E., Zimmel, D., Lauer, U., Häupl, T., Sieper, J., Backhaus, M., Neumann, C., Hoffmann, U., ... Mashreghi, M-F. (2015). miR-148a is upregulated by Twist1 and T-bet and promotes Th1-cell survival by regulating the proapoptotic gene Bim. EUR J IMMUNOL, 45(4), 1192-205. https://doi.org/10.1002/eji.201444633

Vancouver

Haftmann C, Stittrich A-B, Zimmermann J, Fang Z, Hradilkova K, Bardua M et al. miR-148a is upregulated by Twist1 and T-bet and promotes Th1-cell survival by regulating the proapoptotic gene Bim. EUR J IMMUNOL. 2015 Apr;45(4):1192-205. https://doi.org/10.1002/eji.201444633

Bibtex

@article{d7419afbe1f04053947fdd1fe986cd0d,
title = "miR-148a is upregulated by Twist1 and T-bet and promotes Th1-cell survival by regulating the proapoptotic gene Bim",
abstract = "Repeatedly activated T helper 1 (Th1) cells present during chronic inflammation can efficiently adapt to the inflammatory milieu, for example, by expressing the transcription factor Twist1, which limits the immunopathology caused by Th1 cells. Here, we show that in repeatedly activated murine Th1 cells, Twist1 and T-bet induce expression of microRNA-148a (miR-148a). miR-148a regulates expression of the proapoptotic gene Bim, resulting in a decreased Bim/Bcl2 ratio. Inhibition of miR-148a by antagomirs in repeatedly activated Th1 cells increases the expression of Bim, leading to enhanced apoptosis. Knockdown of Bim expression by siRNA in miR-148a antagomir-treated cells restores viability of the Th1 cells, demonstrating that miR-148a controls survival by regulating Bim expression. Thus, Twist1 and T-bet not only control the differentiation and function of Th1 cells, but also their persistence in chronic inflammation. ",
keywords = "Animals, Apoptosis/genetics, Apoptosis Regulatory Proteins/genetics, Arthritis, Rheumatoid/immunology, Bcl-2-Like Protein 11, Cell Survival/immunology, Cells, Cultured, Gene Expression Regulation, Humans, Membrane Proteins/genetics, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, MicroRNAs/antagonists & inhibitors, Nuclear Proteins/genetics, Proto-Oncogene Proteins/genetics, RNA Interference, RNA, Small Interfering, T-Box Domain Proteins/genetics, Th1 Cells/immunology, Twist-Related Protein 1/genetics",
author = "Claudia Haftmann and Anna-Barbara Stittrich and Jakob Zimmermann and Zhuo Fang and Kristyna Hradilkova and Markus Bardua and Kerstin Westendorf and Heinz, {Gitta A} and Ren{\'e} Riedel and Julia Siede and Katrin Lehmann and Weinberger, {Esther E} and David Zimmel and Uta Lauer and Thomas H{\"a}upl and Joachim Sieper and Marina Backhaus and Christian Neumann and Ute Hoffmann and Martina Porstner and Wei Chen and Gr{\"u}n, {Joachim R} and Ria Baumgrass and Mareen Matz and Max L{\"o}hning and Alexander Scheffold and J{\"u}rgen Wittmann and Hyun-Dong Chang and Nikolaus Rajewsky and Hans-Martin J{\"a}ck and Andreas Radbruch and Mir-Farzin Mashreghi",
note = "{\textcopyright} 2014 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2015",
month = apr,
doi = "10.1002/eji.201444633",
language = "English",
volume = "45",
pages = "1192--205",
journal = "EUR J IMMUNOL",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag GmbH",
number = "4",

}

RIS

TY - JOUR

T1 - miR-148a is upregulated by Twist1 and T-bet and promotes Th1-cell survival by regulating the proapoptotic gene Bim

AU - Haftmann, Claudia

AU - Stittrich, Anna-Barbara

AU - Zimmermann, Jakob

AU - Fang, Zhuo

AU - Hradilkova, Kristyna

AU - Bardua, Markus

AU - Westendorf, Kerstin

AU - Heinz, Gitta A

AU - Riedel, René

AU - Siede, Julia

AU - Lehmann, Katrin

AU - Weinberger, Esther E

AU - Zimmel, David

AU - Lauer, Uta

AU - Häupl, Thomas

AU - Sieper, Joachim

AU - Backhaus, Marina

AU - Neumann, Christian

AU - Hoffmann, Ute

AU - Porstner, Martina

AU - Chen, Wei

AU - Grün, Joachim R

AU - Baumgrass, Ria

AU - Matz, Mareen

AU - Löhning, Max

AU - Scheffold, Alexander

AU - Wittmann, Jürgen

AU - Chang, Hyun-Dong

AU - Rajewsky, Nikolaus

AU - Jäck, Hans-Martin

AU - Radbruch, Andreas

AU - Mashreghi, Mir-Farzin

N1 - © 2014 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2015/4

Y1 - 2015/4

N2 - Repeatedly activated T helper 1 (Th1) cells present during chronic inflammation can efficiently adapt to the inflammatory milieu, for example, by expressing the transcription factor Twist1, which limits the immunopathology caused by Th1 cells. Here, we show that in repeatedly activated murine Th1 cells, Twist1 and T-bet induce expression of microRNA-148a (miR-148a). miR-148a regulates expression of the proapoptotic gene Bim, resulting in a decreased Bim/Bcl2 ratio. Inhibition of miR-148a by antagomirs in repeatedly activated Th1 cells increases the expression of Bim, leading to enhanced apoptosis. Knockdown of Bim expression by siRNA in miR-148a antagomir-treated cells restores viability of the Th1 cells, demonstrating that miR-148a controls survival by regulating Bim expression. Thus, Twist1 and T-bet not only control the differentiation and function of Th1 cells, but also their persistence in chronic inflammation.

AB - Repeatedly activated T helper 1 (Th1) cells present during chronic inflammation can efficiently adapt to the inflammatory milieu, for example, by expressing the transcription factor Twist1, which limits the immunopathology caused by Th1 cells. Here, we show that in repeatedly activated murine Th1 cells, Twist1 and T-bet induce expression of microRNA-148a (miR-148a). miR-148a regulates expression of the proapoptotic gene Bim, resulting in a decreased Bim/Bcl2 ratio. Inhibition of miR-148a by antagomirs in repeatedly activated Th1 cells increases the expression of Bim, leading to enhanced apoptosis. Knockdown of Bim expression by siRNA in miR-148a antagomir-treated cells restores viability of the Th1 cells, demonstrating that miR-148a controls survival by regulating Bim expression. Thus, Twist1 and T-bet not only control the differentiation and function of Th1 cells, but also their persistence in chronic inflammation.

KW - Animals

KW - Apoptosis/genetics

KW - Apoptosis Regulatory Proteins/genetics

KW - Arthritis, Rheumatoid/immunology

KW - Bcl-2-Like Protein 11

KW - Cell Survival/immunology

KW - Cells, Cultured

KW - Gene Expression Regulation

KW - Humans

KW - Membrane Proteins/genetics

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - MicroRNAs/antagonists & inhibitors

KW - Nuclear Proteins/genetics

KW - Proto-Oncogene Proteins/genetics

KW - RNA Interference

KW - RNA, Small Interfering

KW - T-Box Domain Proteins/genetics

KW - Th1 Cells/immunology

KW - Twist-Related Protein 1/genetics

U2 - 10.1002/eji.201444633

DO - 10.1002/eji.201444633

M3 - SCORING: Journal article

C2 - 25486906

VL - 45

SP - 1192

EP - 1205

JO - EUR J IMMUNOL

JF - EUR J IMMUNOL

SN - 0014-2980

IS - 4

ER -