Microsatellite DNA alterations of gastrointestinal stromal tumors are predictive for outcome.
Standard
Microsatellite DNA alterations of gastrointestinal stromal tumors are predictive for outcome. / Schurr, Paulus; Wolter, Stefan; Kaifi, Jussuf; Reichelt, Uta; Kleinhans, Helge; Wachowiak, Robin; Yekebas, Emre; Strate, Tim; Kalinin, Viacheslav; Simon, Ronald; Sauter, Guido; Schaefer, Hansjoerg; Izbicki, Jakob.
in: CLIN CANCER RES, Jahrgang 12, Nr. 17, 17, 2006, S. 5151-5157.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Microsatellite DNA alterations of gastrointestinal stromal tumors are predictive for outcome.
AU - Schurr, Paulus
AU - Wolter, Stefan
AU - Kaifi, Jussuf
AU - Reichelt, Uta
AU - Kleinhans, Helge
AU - Wachowiak, Robin
AU - Yekebas, Emre
AU - Strate, Tim
AU - Kalinin, Viacheslav
AU - Simon, Ronald
AU - Sauter, Guido
AU - Schaefer, Hansjoerg
AU - Izbicki, Jakob
PY - 2006
Y1 - 2006
N2 - PURPOSE: In gastrointestinal stromal tumors (GIST), loss of heterozygosity (LOH) on chromosome 22 and its presumptive biological function has been described. The prognostic value of these and other DNA regions for patient survival remains unclear. EXPERIMENTAL DESIGN: Sixty patients who underwent surgery at our institution between 1992 and 2003 for GIST were histopathologically reclassified by immunohistochemistry and the GIST consensus group criteria 2001. Twenty-one microsatellite loci on chromosomes 3, 9, 13, 17, 18, and 22 were screened for alterations in tumor and healthy DNA. Survival was calculated by Kaplan-Meier plots. RESULTS: Eleven (18.3%) of 60 patients showed metastases at presentation. Thirteen (21.7%) of 60 were high-risk GISTs. LOH was found in all tumors. Twenty-eight (46.7%) of 60 showed more than two LOH in 21 microsatellite marker sites. The frequency of single marker LOH varied from 1.7% to 28.3% among tumors. Frequent LOH was found on chromosomes 22 and 17. The correlation of LOH positivity and the consensus scoring was significant (P=0.005, chi2 test). After a median observation time of 33.3 months (95% confidence interval, 23.9-42.6), overall survival was best for patients with tumors of very low, low, and intermediate risks with only 6 of 36 death events, whereas 14 of 24 high-risk and metastasized patients had died (P
AB - PURPOSE: In gastrointestinal stromal tumors (GIST), loss of heterozygosity (LOH) on chromosome 22 and its presumptive biological function has been described. The prognostic value of these and other DNA regions for patient survival remains unclear. EXPERIMENTAL DESIGN: Sixty patients who underwent surgery at our institution between 1992 and 2003 for GIST were histopathologically reclassified by immunohistochemistry and the GIST consensus group criteria 2001. Twenty-one microsatellite loci on chromosomes 3, 9, 13, 17, 18, and 22 were screened for alterations in tumor and healthy DNA. Survival was calculated by Kaplan-Meier plots. RESULTS: Eleven (18.3%) of 60 patients showed metastases at presentation. Thirteen (21.7%) of 60 were high-risk GISTs. LOH was found in all tumors. Twenty-eight (46.7%) of 60 showed more than two LOH in 21 microsatellite marker sites. The frequency of single marker LOH varied from 1.7% to 28.3% among tumors. Frequent LOH was found on chromosomes 22 and 17. The correlation of LOH positivity and the consensus scoring was significant (P=0.005, chi2 test). After a median observation time of 33.3 months (95% confidence interval, 23.9-42.6), overall survival was best for patients with tumors of very low, low, and intermediate risks with only 6 of 36 death events, whereas 14 of 24 high-risk and metastasized patients had died (P
M3 - SCORING: Zeitschriftenaufsatz
VL - 12
SP - 5151
EP - 5157
JO - CLIN CANCER RES
JF - CLIN CANCER RES
SN - 1078-0432
IS - 17
M1 - 17
ER -
