Microinjection into cultured hippocampal neurons: a straightforward approach for controlled cellular delivery of nucleic acids, peptides and antibodies.
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Microinjection into cultured hippocampal neurons: a straightforward approach for controlled cellular delivery of nucleic acids, peptides and antibodies. / Lappe-Siefke, Corinna; Maas, Christoph; Kneussel, Matthias.
in: J NEUROSCI METH, Jahrgang 175, Nr. 1, 1, 2008, S. 88-95.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Microinjection into cultured hippocampal neurons: a straightforward approach for controlled cellular delivery of nucleic acids, peptides and antibodies.
AU - Lappe-Siefke, Corinna
AU - Maas, Christoph
AU - Kneussel, Matthias
PY - 2008
Y1 - 2008
N2 - Functional studies in neurons often require controllable simultaneous delivery of different molecules to individual cells within networks. Microinjection represents a suitable and alternative method to deliver cDNAs, oligonucleotides, siRNAs, peptides or antibodies for expression, expression knockdown or loss-of-function studies, respectively. Moreover, molecules can be systematically applied to individual neurons in a controlled manner without affecting neighbouring cells. Establishment of microinjection is often complicated and time consuming. Here we describe a simple and reliable protocol for molecular cell biologists to establish injection of various molecules (ng to microg range) to living neurons in a reasonable period of time.
AB - Functional studies in neurons often require controllable simultaneous delivery of different molecules to individual cells within networks. Microinjection represents a suitable and alternative method to deliver cDNAs, oligonucleotides, siRNAs, peptides or antibodies for expression, expression knockdown or loss-of-function studies, respectively. Moreover, molecules can be systematically applied to individual neurons in a controlled manner without affecting neighbouring cells. Establishment of microinjection is often complicated and time consuming. Here we describe a simple and reliable protocol for molecular cell biologists to establish injection of various molecules (ng to microg range) to living neurons in a reasonable period of time.
M3 - SCORING: Zeitschriftenaufsatz
VL - 175
SP - 88
EP - 95
JO - J NEUROSCI METH
JF - J NEUROSCI METH
SN - 0165-0270
IS - 1
M1 - 1
ER -
