Microbiota of Children With Complex Appendicitis
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Microbiota of Children With Complex Appendicitis : Different Composition and Diversity of The Microbiota in Children With Complex Compared With Simple Appendicitis. / The, Sarah-May M L; Bakx, Roel; Budding, Andries E; de Meij, Tim G J; van der Lee, Johanna H; Bunders, Madeleine J; Poort, Linda; Heij, Hugo A; van Heurn, L W Ernst; Gorter, Ramon R.
in: PEDIATR INFECT DIS J, Jahrgang 38, Nr. 10, 10.2019, S. 1054-1060.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Microbiota of Children With Complex Appendicitis
T2 - Different Composition and Diversity of The Microbiota in Children With Complex Compared With Simple Appendicitis
AU - The, Sarah-May M L
AU - Bakx, Roel
AU - Budding, Andries E
AU - de Meij, Tim G J
AU - van der Lee, Johanna H
AU - Bunders, Madeleine J
AU - Poort, Linda
AU - Heij, Hugo A
AU - van Heurn, L W Ernst
AU - Gorter, Ramon R
PY - 2019/10
Y1 - 2019/10
N2 - BACKGROUND: Two types of appendicitis are hypothesized, simple and complex, with potential different treatment strategies. To improve differentiation, underlying pathogeneses need to be further unraveled.AIM: To determine if the microbial composition in the appendix differs between children with simple and complex appendicitis.METHODS: Two-center, prospective cohort study including 40 children (0-17 years old) undergoing appendectomy for suspected appendicitis. Appendix tissue was used for IS-pro analysis to identify bacterial species by their length of 16S-23S rDNA interspacer (IS) region. Cluster analysis, based on IS-profiles, and correspondence with type of appendicitis, using Fisher exact test, was performed. Simple and complex appendicitis were compared regarding bacterial presence, intensity and diversity, using Fisher exact test and Mann-Whitney U test, respectively.RESULTS: Appendicitis was confirmed in 36 of 40 patients (16 simple, 20 complex). Cluster analysis identified 2 clusters, encompassing 34 patients. Distribution of simple and complex appendicitis was 12 (80%) and 3 (20%) versus 3 (16%) and 16 (84%) patients for clusters 1 and 2, respectively (P < 0.001). Complex appendicitis was on phylum level characterized by an increased intensity (Bacteroidetes P = 0.001, Firmicutes, Actinobacteria, Fusobacteria and Verrucomicrobia (FAFV) P = 0.005 and Proteobacteria P < 0.001) and diversity (Bacteroidetes P = 0.001 and Proteobacteria P = 0.016) and an increased abundance of 5 species (Alistipes finegoldii P = 0.009, Bacteroides fragilis P = 0.002, Escherichia coli P = 0.014, Parvimonas micra P = 0.022 and Sutterella spp P = 0.026).CONCLUSIONS: The microbial composition of the appendix differs between children with simple and complex appendicitis, regarding both composition and diversity. Future research should focus on the role of these bacteria in the pathogenesis of both types and its implications for preoperative diagnostics.
AB - BACKGROUND: Two types of appendicitis are hypothesized, simple and complex, with potential different treatment strategies. To improve differentiation, underlying pathogeneses need to be further unraveled.AIM: To determine if the microbial composition in the appendix differs between children with simple and complex appendicitis.METHODS: Two-center, prospective cohort study including 40 children (0-17 years old) undergoing appendectomy for suspected appendicitis. Appendix tissue was used for IS-pro analysis to identify bacterial species by their length of 16S-23S rDNA interspacer (IS) region. Cluster analysis, based on IS-profiles, and correspondence with type of appendicitis, using Fisher exact test, was performed. Simple and complex appendicitis were compared regarding bacterial presence, intensity and diversity, using Fisher exact test and Mann-Whitney U test, respectively.RESULTS: Appendicitis was confirmed in 36 of 40 patients (16 simple, 20 complex). Cluster analysis identified 2 clusters, encompassing 34 patients. Distribution of simple and complex appendicitis was 12 (80%) and 3 (20%) versus 3 (16%) and 16 (84%) patients for clusters 1 and 2, respectively (P < 0.001). Complex appendicitis was on phylum level characterized by an increased intensity (Bacteroidetes P = 0.001, Firmicutes, Actinobacteria, Fusobacteria and Verrucomicrobia (FAFV) P = 0.005 and Proteobacteria P < 0.001) and diversity (Bacteroidetes P = 0.001 and Proteobacteria P = 0.016) and an increased abundance of 5 species (Alistipes finegoldii P = 0.009, Bacteroides fragilis P = 0.002, Escherichia coli P = 0.014, Parvimonas micra P = 0.022 and Sutterella spp P = 0.026).CONCLUSIONS: The microbial composition of the appendix differs between children with simple and complex appendicitis, regarding both composition and diversity. Future research should focus on the role of these bacteria in the pathogenesis of both types and its implications for preoperative diagnostics.
KW - Adolescent
KW - Appendectomy
KW - Appendicitis/microbiology
KW - Appendix/microbiology
KW - Bacteria/classification
KW - Child
KW - Child, Preschool
KW - Cluster Analysis
KW - DNA, Bacterial/chemistry
KW - DNA, Ribosomal/chemistry
KW - Female
KW - Humans
KW - Infant
KW - Infant, Newborn
KW - Male
KW - Microbiota
KW - Phylogeny
KW - Pilot Projects
KW - Prospective Studies
KW - RNA, Ribosomal, 16S/genetics
KW - RNA, Ribosomal, 23S/genetics
KW - Sequence Analysis, DNA
U2 - 10.1097/INF.0000000000002434
DO - 10.1097/INF.0000000000002434
M3 - SCORING: Journal article
C2 - 31568143
VL - 38
SP - 1054
EP - 1060
JO - PEDIATR INFECT DIS J
JF - PEDIATR INFECT DIS J
SN - 0891-3668
IS - 10
ER -
