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Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo

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Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo. / Rivera-Monroy, Jhon; Musiol, Lena; Unthan-Fechner, Kirsten; Farkas, Ákos; Clancy, Anne; Coy-Vergara, Javier; Weill, Uri; Gockel, Sarah; Lin, Shuh-Yow; Corey, David P; Kohl, Tobias; Ströbel, Philipp; Schuldiner, Maya; Schwappach, Blanche; Vilardi, Fabio.

in: SCI REP-UK, Jahrgang 6, 21.12.2016, S. 39464.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Rivera-Monroy, J, Musiol, L, Unthan-Fechner, K, Farkas, Á, Clancy, A, Coy-Vergara, J, Weill, U, Gockel, S, Lin, S-Y, Corey, DP, Kohl, T, Ströbel, P, Schuldiner, M, Schwappach, B & Vilardi, F 2016, 'Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo', SCI REP-UK, Jg. 6, S. 39464. https://doi.org/10.1038/srep39464

APA

Rivera-Monroy, J., Musiol, L., Unthan-Fechner, K., Farkas, Á., Clancy, A., Coy-Vergara, J., Weill, U., Gockel, S., Lin, S-Y., Corey, D. P., Kohl, T., Ströbel, P., Schuldiner, M., Schwappach, B., & Vilardi, F. (2016). Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo. SCI REP-UK, 6, 39464. https://doi.org/10.1038/srep39464

Vancouver

Rivera-Monroy J, Musiol L, Unthan-Fechner K, Farkas Á, Clancy A, Coy-Vergara J et al. Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo. SCI REP-UK. 2016 Dez 21;6:39464. https://doi.org/10.1038/srep39464

Bibtex

@article{ff9046f16ae04e828de228503a35b107,
title = "Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo",
abstract = "Tail-anchored (TA) proteins are post-translationally inserted into membranes. The TRC40 pathway targets TA proteins to the endoplasmic reticulum via a receptor comprised of WRB and CAML. TRC40 pathway clients have been identified using in vitro assays, however, the relevance of the TRC40 pathway in vivo remains unknown. We followed the fate of TA proteins in two tissue-specific WRB knockout mouse models and found that their dependence on the TRC40 pathway in vitro did not predict their reaction to receptor depletion in vivo. The SNARE syntaxin 5 (Stx5) was extremely sensitive to disruption of the TRC40 pathway. Screening yeast TA proteins with mammalian homologues, we show that the particular sensitivity of Stx5 is conserved, possibly due to aggregation propensity of its cytoplasmic domain. We establish that Stx5 is an autophagy target that is inefficiently membrane-targeted by alternative pathways. Our results highlight an intimate relationship between the TRC40 pathway and cellular proteostasis.",
keywords = "Adaptor Proteins, Signal Transducing/genetics, Adenosine Triphosphatases/metabolism, Alleles, Animals, Autophagy, Cytoplasm/metabolism, HeLa Cells, Humans, Membrane Proteins/genetics, Mice, Mice, Knockout, Myocytes, Cardiac/cytology, Proteasome Endopeptidase Complex/metabolism, Protein Domains, Proteostasis, Qa-SNARE Proteins/metabolism, RNA, Small Interfering/metabolism",
author = "Jhon Rivera-Monroy and Lena Musiol and Kirsten Unthan-Fechner and {\'A}kos Farkas and Anne Clancy and Javier Coy-Vergara and Uri Weill and Sarah Gockel and Shuh-Yow Lin and Corey, {David P} and Tobias Kohl and Philipp Str{\"o}bel and Maya Schuldiner and Blanche Schwappach and Fabio Vilardi",
year = "2016",
month = dec,
day = "21",
doi = "10.1038/srep39464",
language = "English",
volume = "6",
pages = "39464",
journal = "SCI REP-UK",
issn = "2045-2322",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - Mice lacking WRB reveal differential biogenesis requirements of tail-anchored proteins in vivo

AU - Rivera-Monroy, Jhon

AU - Musiol, Lena

AU - Unthan-Fechner, Kirsten

AU - Farkas, Ákos

AU - Clancy, Anne

AU - Coy-Vergara, Javier

AU - Weill, Uri

AU - Gockel, Sarah

AU - Lin, Shuh-Yow

AU - Corey, David P

AU - Kohl, Tobias

AU - Ströbel, Philipp

AU - Schuldiner, Maya

AU - Schwappach, Blanche

AU - Vilardi, Fabio

PY - 2016/12/21

Y1 - 2016/12/21

N2 - Tail-anchored (TA) proteins are post-translationally inserted into membranes. The TRC40 pathway targets TA proteins to the endoplasmic reticulum via a receptor comprised of WRB and CAML. TRC40 pathway clients have been identified using in vitro assays, however, the relevance of the TRC40 pathway in vivo remains unknown. We followed the fate of TA proteins in two tissue-specific WRB knockout mouse models and found that their dependence on the TRC40 pathway in vitro did not predict their reaction to receptor depletion in vivo. The SNARE syntaxin 5 (Stx5) was extremely sensitive to disruption of the TRC40 pathway. Screening yeast TA proteins with mammalian homologues, we show that the particular sensitivity of Stx5 is conserved, possibly due to aggregation propensity of its cytoplasmic domain. We establish that Stx5 is an autophagy target that is inefficiently membrane-targeted by alternative pathways. Our results highlight an intimate relationship between the TRC40 pathway and cellular proteostasis.

AB - Tail-anchored (TA) proteins are post-translationally inserted into membranes. The TRC40 pathway targets TA proteins to the endoplasmic reticulum via a receptor comprised of WRB and CAML. TRC40 pathway clients have been identified using in vitro assays, however, the relevance of the TRC40 pathway in vivo remains unknown. We followed the fate of TA proteins in two tissue-specific WRB knockout mouse models and found that their dependence on the TRC40 pathway in vitro did not predict their reaction to receptor depletion in vivo. The SNARE syntaxin 5 (Stx5) was extremely sensitive to disruption of the TRC40 pathway. Screening yeast TA proteins with mammalian homologues, we show that the particular sensitivity of Stx5 is conserved, possibly due to aggregation propensity of its cytoplasmic domain. We establish that Stx5 is an autophagy target that is inefficiently membrane-targeted by alternative pathways. Our results highlight an intimate relationship between the TRC40 pathway and cellular proteostasis.

KW - Adaptor Proteins, Signal Transducing/genetics

KW - Adenosine Triphosphatases/metabolism

KW - Alleles

KW - Animals

KW - Autophagy

KW - Cytoplasm/metabolism

KW - HeLa Cells

KW - Humans

KW - Membrane Proteins/genetics

KW - Mice

KW - Mice, Knockout

KW - Myocytes, Cardiac/cytology

KW - Proteasome Endopeptidase Complex/metabolism

KW - Protein Domains

KW - Proteostasis

KW - Qa-SNARE Proteins/metabolism

KW - RNA, Small Interfering/metabolism

U2 - 10.1038/srep39464

DO - 10.1038/srep39464

M3 - SCORING: Journal article

C2 - 28000760

VL - 6

SP - 39464

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

ER -