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MIC-Drop: A platform for large-scale in vivo CRISPR screens

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MIC-Drop: A platform for large-scale in vivo CRISPR screens. / Parvez, Saba; Herdman, Chelsea; Beerens, Manu; Chakraborti, Korak; Harmer, Zachary P; Yeh, Jing-Ruey J; MacRae, Calum A; Yost, H Joseph; Peterson, Randall T.

in: SCIENCE, Jahrgang 373, Nr. 6559, 03.09.2021, S. 1146-1151.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Parvez, S, Herdman, C, Beerens, M, Chakraborti, K, Harmer, ZP, Yeh, J-RJ, MacRae, CA, Yost, HJ & Peterson, RT 2021, 'MIC-Drop: A platform for large-scale in vivo CRISPR screens', SCIENCE, Jg. 373, Nr. 6559, S. 1146-1151. https://doi.org/10.1126/science.abi8870

APA

Parvez, S., Herdman, C., Beerens, M., Chakraborti, K., Harmer, Z. P., Yeh, J-R. J., MacRae, C. A., Yost, H. J., & Peterson, R. T. (2021). MIC-Drop: A platform for large-scale in vivo CRISPR screens. SCIENCE, 373(6559), 1146-1151. https://doi.org/10.1126/science.abi8870

Vancouver

Parvez S, Herdman C, Beerens M, Chakraborti K, Harmer ZP, Yeh J-RJ et al. MIC-Drop: A platform for large-scale in vivo CRISPR screens. SCIENCE. 2021 Sep 3;373(6559):1146-1151. https://doi.org/10.1126/science.abi8870

Bibtex

@article{5c3d00d801564bbdab3410919362eaeb,
title = "MIC-Drop: A platform for large-scale in vivo CRISPR screens",
abstract = "CRISPR-Cas9 can be scaled up for large-scale screens in cultured cells, but CRISPR screens in animals have been challenging because generating, validating, and keeping track of large numbers of mutant animals is prohibitive. Here, we introduce Multiplexed Intermixed CRISPR Droplets (MIC-Drop), a platform combining droplet microfluidics, single-needle en masse CRISPR ribonucleoprotein injections, and DNA barcoding to enable large-scale functional genetic screens in zebrafish. The platform can efficiently identify genes responsible for morphological or behavioral phenotypes. In one application, we showed that MIC-Drop could identify small-molecule targets. Furthermore, in a MIC-Drop screen of 188 poorly characterized genes, we discovered several genes important for cardiac development and function. With the potential to scale to thousands of genes, MIC-Drop enables genome-scale reverse genetic screens in model organisms.",
keywords = "Animals, CRISPR-Cas Systems, Cardiovascular System/growth & development, Cell Culture Techniques, Genetic Testing, High-Throughput Nucleotide Sequencing, Microfluidic Analytical Techniques, Zebrafish/genetics",
author = "Saba Parvez and Chelsea Herdman and Manu Beerens and Korak Chakraborti and Harmer, {Zachary P} and Yeh, {Jing-Ruey J} and MacRae, {Calum A} and Yost, {H Joseph} and Peterson, {Randall T}",
year = "2021",
month = sep,
day = "3",
doi = "10.1126/science.abi8870",
language = "English",
volume = "373",
pages = "1146--1151",
journal = "SCIENCE",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6559",

}

RIS

TY - JOUR

T1 - MIC-Drop: A platform for large-scale in vivo CRISPR screens

AU - Parvez, Saba

AU - Herdman, Chelsea

AU - Beerens, Manu

AU - Chakraborti, Korak

AU - Harmer, Zachary P

AU - Yeh, Jing-Ruey J

AU - MacRae, Calum A

AU - Yost, H Joseph

AU - Peterson, Randall T

PY - 2021/9/3

Y1 - 2021/9/3

N2 - CRISPR-Cas9 can be scaled up for large-scale screens in cultured cells, but CRISPR screens in animals have been challenging because generating, validating, and keeping track of large numbers of mutant animals is prohibitive. Here, we introduce Multiplexed Intermixed CRISPR Droplets (MIC-Drop), a platform combining droplet microfluidics, single-needle en masse CRISPR ribonucleoprotein injections, and DNA barcoding to enable large-scale functional genetic screens in zebrafish. The platform can efficiently identify genes responsible for morphological or behavioral phenotypes. In one application, we showed that MIC-Drop could identify small-molecule targets. Furthermore, in a MIC-Drop screen of 188 poorly characterized genes, we discovered several genes important for cardiac development and function. With the potential to scale to thousands of genes, MIC-Drop enables genome-scale reverse genetic screens in model organisms.

AB - CRISPR-Cas9 can be scaled up for large-scale screens in cultured cells, but CRISPR screens in animals have been challenging because generating, validating, and keeping track of large numbers of mutant animals is prohibitive. Here, we introduce Multiplexed Intermixed CRISPR Droplets (MIC-Drop), a platform combining droplet microfluidics, single-needle en masse CRISPR ribonucleoprotein injections, and DNA barcoding to enable large-scale functional genetic screens in zebrafish. The platform can efficiently identify genes responsible for morphological or behavioral phenotypes. In one application, we showed that MIC-Drop could identify small-molecule targets. Furthermore, in a MIC-Drop screen of 188 poorly characterized genes, we discovered several genes important for cardiac development and function. With the potential to scale to thousands of genes, MIC-Drop enables genome-scale reverse genetic screens in model organisms.

KW - Animals

KW - CRISPR-Cas Systems

KW - Cardiovascular System/growth & development

KW - Cell Culture Techniques

KW - Genetic Testing

KW - High-Throughput Nucleotide Sequencing

KW - Microfluidic Analytical Techniques

KW - Zebrafish/genetics

U2 - 10.1126/science.abi8870

DO - 10.1126/science.abi8870

M3 - SCORING: Journal article

C2 - 34413171

VL - 373

SP - 1146

EP - 1151

JO - SCIENCE

JF - SCIENCE

SN - 0036-8075

IS - 6559

ER -