Methylation-specific PCR for DNA-based detection of occult tumor cells in lymph nodes of prostate cancer patients.
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Methylation-specific PCR for DNA-based detection of occult tumor cells in lymph nodes of prostate cancer patients. / Köllermann, Jens; Müller, Markus; Goessl, Carsten; Krause, Hans; Helpap, Burkhard; Pantel, Klaus; Miller, Kurt.
in: EUR UROL, Jahrgang 44, Nr. 5, 5, 2003, S. 533-538.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Methylation-specific PCR for DNA-based detection of occult tumor cells in lymph nodes of prostate cancer patients.
AU - Köllermann, Jens
AU - Müller, Markus
AU - Goessl, Carsten
AU - Krause, Hans
AU - Helpap, Burkhard
AU - Pantel, Klaus
AU - Miller, Kurt
PY - 2003
Y1 - 2003
N2 - OBJECTIVES: To assess the feasibility of methylation-specific PCR (MSP) for the detection of promoter hypermethylation of the detoxifying glutathione-S-transferase P1 gene (GSTP1) to detect occult prostate cancer cells in lymph nodes (LNs). METHODS: Paraffin-embedded pelvic LNs from 20 patients with pT2pN0M0R0 prostate cancer who developed PSA relapse were assessed by MSP. In 18 of the patients, samples of the primary tumor were obtained for MSP. In 19 patients, bone marrow (BM) aspirates were analyzed preoperatively for disseminated tumor cells by immunocytochemistry (mAb A45-B/B3). In 16 patients, biopsies of the anastomotic region were performed following PSA relapse. As a negative control GSTP1 methylation status was also assessed in LNs from 9 patients for whom an autopsy was performed for non-cancer-related causes. RESULTS: All primary tumors displayed GSTP1 hypermethylation (HM). Preoperative BM assessment showed disseminated tumor cells in 8/20 cases (40%). In 4 patients, biopsies of the vesico-urethral anastomosis showed local tumor recurrence. The LNs in the cancer patients showed GSTP1 HM in 18/20 cases (90%) versus 1/9 patients (11.1%) in the non-cancer cohort (p
AB - OBJECTIVES: To assess the feasibility of methylation-specific PCR (MSP) for the detection of promoter hypermethylation of the detoxifying glutathione-S-transferase P1 gene (GSTP1) to detect occult prostate cancer cells in lymph nodes (LNs). METHODS: Paraffin-embedded pelvic LNs from 20 patients with pT2pN0M0R0 prostate cancer who developed PSA relapse were assessed by MSP. In 18 of the patients, samples of the primary tumor were obtained for MSP. In 19 patients, bone marrow (BM) aspirates were analyzed preoperatively for disseminated tumor cells by immunocytochemistry (mAb A45-B/B3). In 16 patients, biopsies of the anastomotic region were performed following PSA relapse. As a negative control GSTP1 methylation status was also assessed in LNs from 9 patients for whom an autopsy was performed for non-cancer-related causes. RESULTS: All primary tumors displayed GSTP1 hypermethylation (HM). Preoperative BM assessment showed disseminated tumor cells in 8/20 cases (40%). In 4 patients, biopsies of the vesico-urethral anastomosis showed local tumor recurrence. The LNs in the cancer patients showed GSTP1 HM in 18/20 cases (90%) versus 1/9 patients (11.1%) in the non-cancer cohort (p
M3 - SCORING: Zeitschriftenaufsatz
VL - 44
SP - 533
EP - 538
JO - EUR UROL
JF - EUR UROL
SN - 0302-2838
IS - 5
M1 - 5
ER -