Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion
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Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion. / Horstmann, Julia A; Lunelli, Michele; Cazzola, Hélène; Heidemann, Johannes; Kühne, Caroline; Steffen, Pascal; Szefs, Sandra; Rossi, Claire; Lokareddy, Ravi K; Wang, Chu; Lemaire, Laurine; Hughes, Kelly T; Uetrecht, Charlotte; Schlüter, Hartmut; Grassl, Guntram A; Stradal, Theresia E B; Rossez, Yannick; Kolbe, Michael; Erhardt, Marc.
in: NAT COMMUN, Jahrgang 11, Nr. 1, 24.04.2020, S. 2013.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion
AU - Horstmann, Julia A
AU - Lunelli, Michele
AU - Cazzola, Hélène
AU - Heidemann, Johannes
AU - Kühne, Caroline
AU - Steffen, Pascal
AU - Szefs, Sandra
AU - Rossi, Claire
AU - Lokareddy, Ravi K
AU - Wang, Chu
AU - Lemaire, Laurine
AU - Hughes, Kelly T
AU - Uetrecht, Charlotte
AU - Schlüter, Hartmut
AU - Grassl, Guntram A
AU - Stradal, Theresia E B
AU - Rossez, Yannick
AU - Kolbe, Michael
AU - Erhardt, Marc
PY - 2020/4/24
Y1 - 2020/4/24
N2 - The long external filament of bacterial flagella is composed of several thousand copies of a single protein, flagellin. Here, we explore the role played by lysine methylation of flagellin in Salmonella, which requires the methylase FliB. We show that both flagellins of Salmonella enterica serovar Typhimurium, FliC and FljB, are methylated at surface-exposed lysine residues by FliB. A Salmonella Typhimurium mutant deficient in flagellin methylation is outcompeted for gut colonization in a gastroenteritis mouse model, and methylation of flagellin promotes bacterial invasion of epithelial cells in vitro. Lysine methylation increases the surface hydrophobicity of flagellin, and enhances flagella-dependent adhesion of Salmonella to phosphatidylcholine vesicles and epithelial cells. Therefore, posttranslational methylation of flagellin facilitates adhesion of Salmonella Typhimurium to hydrophobic host cell surfaces, and contributes to efficient gut colonization and host infection.
AB - The long external filament of bacterial flagella is composed of several thousand copies of a single protein, flagellin. Here, we explore the role played by lysine methylation of flagellin in Salmonella, which requires the methylase FliB. We show that both flagellins of Salmonella enterica serovar Typhimurium, FliC and FljB, are methylated at surface-exposed lysine residues by FliB. A Salmonella Typhimurium mutant deficient in flagellin methylation is outcompeted for gut colonization in a gastroenteritis mouse model, and methylation of flagellin promotes bacterial invasion of epithelial cells in vitro. Lysine methylation increases the surface hydrophobicity of flagellin, and enhances flagella-dependent adhesion of Salmonella to phosphatidylcholine vesicles and epithelial cells. Therefore, posttranslational methylation of flagellin facilitates adhesion of Salmonella Typhimurium to hydrophobic host cell surfaces, and contributes to efficient gut colonization and host infection.
U2 - 10.1038/s41467-020-15738-3
DO - 10.1038/s41467-020-15738-3
M3 - SCORING: Journal article
C2 - 32332720
VL - 11
SP - 2013
JO - NAT COMMUN
JF - NAT COMMUN
SN - 2041-1723
IS - 1
ER -