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MerMAIDs: a family of metagenomically discovered marine anion-conducting and intensely desensitizing channelrhodopsins

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MerMAIDs: a family of metagenomically discovered marine anion-conducting and intensely desensitizing channelrhodopsins. / Oppermann, Johannes; Fischer, Paul; Silapetere, Arita; Liepe, Bernhard; Rodriguez-Rozada, Silvia; Flores-Uribe, José; Peter, Enrico; Keidel, Anke; Vierock, Johannes; Kaufmann, Joel; Broser, Matthias; Luck, Meike; Bartl, Franz; Hildebrandt, Peter; Wiegert, J Simon; Béjà, Oded; Hegemann, Peter; Wietek, Jonas.

in: NAT COMMUN, Jahrgang 10, Nr. 1, 25.07.2019, S. 3315.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Oppermann, J, Fischer, P, Silapetere, A, Liepe, B, Rodriguez-Rozada, S, Flores-Uribe, J, Peter, E, Keidel, A, Vierock, J, Kaufmann, J, Broser, M, Luck, M, Bartl, F, Hildebrandt, P, Wiegert, JS, Béjà, O, Hegemann, P & Wietek, J 2019, 'MerMAIDs: a family of metagenomically discovered marine anion-conducting and intensely desensitizing channelrhodopsins', NAT COMMUN, Jg. 10, Nr. 1, S. 3315. https://doi.org/10.1038/s41467-019-11322-6

APA

Oppermann, J., Fischer, P., Silapetere, A., Liepe, B., Rodriguez-Rozada, S., Flores-Uribe, J., Peter, E., Keidel, A., Vierock, J., Kaufmann, J., Broser, M., Luck, M., Bartl, F., Hildebrandt, P., Wiegert, J. S., Béjà, O., Hegemann, P., & Wietek, J. (2019). MerMAIDs: a family of metagenomically discovered marine anion-conducting and intensely desensitizing channelrhodopsins. NAT COMMUN, 10(1), 3315. https://doi.org/10.1038/s41467-019-11322-6

Vancouver

Oppermann J, Fischer P, Silapetere A, Liepe B, Rodriguez-Rozada S, Flores-Uribe J et al. MerMAIDs: a family of metagenomically discovered marine anion-conducting and intensely desensitizing channelrhodopsins. NAT COMMUN. 2019 Jul 25;10(1):3315. https://doi.org/10.1038/s41467-019-11322-6

Bibtex

@article{7265c86d17794e668c87688c9f920cc8,
title = "MerMAIDs: a family of metagenomically discovered marine anion-conducting and intensely desensitizing channelrhodopsins",
abstract = "Channelrhodopsins (ChRs) are algal light-gated ion channels widely used as optogenetic tools for manipulating neuronal activity. ChRs desensitize under continuous bright-light illumination, resulting in a significant decline of photocurrents. Here we describe a metagenomically identified family of phylogenetically distinct anion-conducting ChRs (designated MerMAIDs). MerMAIDs almost completely desensitize during continuous illumination due to accumulation of a late non-conducting photointermediate that disrupts the ion permeation pathway. MerMAID desensitization can be fully explained by a single photocycle in which a long-lived desensitized state follows the short-lived conducting state. A conserved cysteine is the critical factor in desensitization, as its mutation results in recovery of large stationary photocurrents. The rapid desensitization of MerMAIDs enables their use as optogenetic silencers for transient suppression of individual action potentials without affecting subsequent spiking during continuous illumination. Our results could facilitate the development of optogenetic tools from metagenomic databases and enhance general understanding of ChR function.",
author = "Johannes Oppermann and Paul Fischer and Arita Silapetere and Bernhard Liepe and Silvia Rodriguez-Rozada and Jos{\'e} Flores-Uribe and Enrico Peter and Anke Keidel and Johannes Vierock and Joel Kaufmann and Matthias Broser and Meike Luck and Franz Bartl and Peter Hildebrandt and Wiegert, {J Simon} and Oded B{\'e}j{\`a} and Peter Hegemann and Jonas Wietek",
year = "2019",
month = jul,
day = "25",
doi = "10.1038/s41467-019-11322-6",
language = "English",
volume = "10",
pages = "3315",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - MerMAIDs: a family of metagenomically discovered marine anion-conducting and intensely desensitizing channelrhodopsins

AU - Oppermann, Johannes

AU - Fischer, Paul

AU - Silapetere, Arita

AU - Liepe, Bernhard

AU - Rodriguez-Rozada, Silvia

AU - Flores-Uribe, José

AU - Peter, Enrico

AU - Keidel, Anke

AU - Vierock, Johannes

AU - Kaufmann, Joel

AU - Broser, Matthias

AU - Luck, Meike

AU - Bartl, Franz

AU - Hildebrandt, Peter

AU - Wiegert, J Simon

AU - Béjà, Oded

AU - Hegemann, Peter

AU - Wietek, Jonas

PY - 2019/7/25

Y1 - 2019/7/25

N2 - Channelrhodopsins (ChRs) are algal light-gated ion channels widely used as optogenetic tools for manipulating neuronal activity. ChRs desensitize under continuous bright-light illumination, resulting in a significant decline of photocurrents. Here we describe a metagenomically identified family of phylogenetically distinct anion-conducting ChRs (designated MerMAIDs). MerMAIDs almost completely desensitize during continuous illumination due to accumulation of a late non-conducting photointermediate that disrupts the ion permeation pathway. MerMAID desensitization can be fully explained by a single photocycle in which a long-lived desensitized state follows the short-lived conducting state. A conserved cysteine is the critical factor in desensitization, as its mutation results in recovery of large stationary photocurrents. The rapid desensitization of MerMAIDs enables their use as optogenetic silencers for transient suppression of individual action potentials without affecting subsequent spiking during continuous illumination. Our results could facilitate the development of optogenetic tools from metagenomic databases and enhance general understanding of ChR function.

AB - Channelrhodopsins (ChRs) are algal light-gated ion channels widely used as optogenetic tools for manipulating neuronal activity. ChRs desensitize under continuous bright-light illumination, resulting in a significant decline of photocurrents. Here we describe a metagenomically identified family of phylogenetically distinct anion-conducting ChRs (designated MerMAIDs). MerMAIDs almost completely desensitize during continuous illumination due to accumulation of a late non-conducting photointermediate that disrupts the ion permeation pathway. MerMAID desensitization can be fully explained by a single photocycle in which a long-lived desensitized state follows the short-lived conducting state. A conserved cysteine is the critical factor in desensitization, as its mutation results in recovery of large stationary photocurrents. The rapid desensitization of MerMAIDs enables their use as optogenetic silencers for transient suppression of individual action potentials without affecting subsequent spiking during continuous illumination. Our results could facilitate the development of optogenetic tools from metagenomic databases and enhance general understanding of ChR function.

U2 - 10.1038/s41467-019-11322-6

DO - 10.1038/s41467-019-11322-6

M3 - SCORING: Journal article

C2 - 31346176

VL - 10

SP - 3315

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -