Mechanism of AAA+ ATPase-mediated RuvAB-Holliday junction branch migration
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Mechanism of AAA+ ATPase-mediated RuvAB-Holliday junction branch migration. / Wald, Jiri; Fahrenkamp, Dirk; Goessweiner-Mohr, Nikolaus; Lugmayr, Wolfgang; Ciccarelli, Luciano; Vesper, Oliver; Marlovits, Thomas C.
in: NATURE, Jahrgang 609, Nr. 7927, 09.2022, S. 630-639.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Mechanism of AAA+ ATPase-mediated RuvAB-Holliday junction branch migration
AU - Wald, Jiri
AU - Fahrenkamp, Dirk
AU - Goessweiner-Mohr, Nikolaus
AU - Lugmayr, Wolfgang
AU - Ciccarelli, Luciano
AU - Vesper, Oliver
AU - Marlovits, Thomas C
N1 - © 2022. The Author(s).
PY - 2022/9
Y1 - 2022/9
N2 - The Holliday junction is a key intermediate formed during DNA recombination across all kingdoms of life1. In bacteria, the Holliday junction is processed by two homo-hexameric AAA+ ATPase RuvB motors, which assemble together with the RuvA-Holliday junction complex to energize the strand-exchange reaction2. Despite its importance for chromosome maintenance, the structure and mechanism by which this complex facilitates branch migration are unknown. Here, using time-resolved cryo-electron microscopy, we obtained structures of the ATP-hydrolysing RuvAB complex in seven distinct conformational states, captured during assembly and processing of a Holliday junction. Five structures together resolve the complete nucleotide cycle and reveal the spatiotemporal relationship between ATP hydrolysis, nucleotide exchange and context-specific conformational changes in RuvB. Coordinated motions in a converter formed by DNA-disengaged RuvB subunits stimulate hydrolysis and nucleotide exchange. Immobilization of the converter enables RuvB to convert the ATP-contained energy into a lever motion, which generates the pulling force driving the branch migration. We show that RuvB motors rotate together with the DNA substrate, which, together with a progressing nucleotide cycle, forms the mechanistic basis for DNA recombination by continuous branch migration. Together, our data decipher the molecular principles of homologous recombination by the RuvAB complex, elucidate discrete and sequential transition-state intermediates for chemo-mechanical coupling of hexameric AAA+ motors and provide a blueprint for the design of state-specific compounds targeting AAA+ motors.
AB - The Holliday junction is a key intermediate formed during DNA recombination across all kingdoms of life1. In bacteria, the Holliday junction is processed by two homo-hexameric AAA+ ATPase RuvB motors, which assemble together with the RuvA-Holliday junction complex to energize the strand-exchange reaction2. Despite its importance for chromosome maintenance, the structure and mechanism by which this complex facilitates branch migration are unknown. Here, using time-resolved cryo-electron microscopy, we obtained structures of the ATP-hydrolysing RuvAB complex in seven distinct conformational states, captured during assembly and processing of a Holliday junction. Five structures together resolve the complete nucleotide cycle and reveal the spatiotemporal relationship between ATP hydrolysis, nucleotide exchange and context-specific conformational changes in RuvB. Coordinated motions in a converter formed by DNA-disengaged RuvB subunits stimulate hydrolysis and nucleotide exchange. Immobilization of the converter enables RuvB to convert the ATP-contained energy into a lever motion, which generates the pulling force driving the branch migration. We show that RuvB motors rotate together with the DNA substrate, which, together with a progressing nucleotide cycle, forms the mechanistic basis for DNA recombination by continuous branch migration. Together, our data decipher the molecular principles of homologous recombination by the RuvAB complex, elucidate discrete and sequential transition-state intermediates for chemo-mechanical coupling of hexameric AAA+ motors and provide a blueprint for the design of state-specific compounds targeting AAA+ motors.
KW - ATPases Associated with Diverse Cellular Activities/genetics
KW - Adenosine Triphosphate
KW - Bacterial Proteins/metabolism
KW - Cryoelectron Microscopy
KW - DNA
KW - DNA Helicases/genetics
KW - DNA, Cruciform
KW - DNA-Binding Proteins/metabolism
KW - Escherichia coli/genetics
KW - Escherichia coli Proteins/genetics
U2 - 10.1038/s41586-022-05121-1
DO - 10.1038/s41586-022-05121-1
M3 - SCORING: Journal article
C2 - 36002576
VL - 609
SP - 630
EP - 639
JO - NATURE
JF - NATURE
SN - 0028-0836
IS - 7927
ER -