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Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes

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Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes. / Saul, Meike J; Hegewald, Anett B; Emmerich, Anne C; Ossipova, Elena; Vogel, Marc; Baumann, Isabell; Kultima, Kim; Lengqivst, Johan; Steinhilber, Dieter; Jakobsson, Per Johan.

in: FRONT PHARMACOL, Jahrgang 10, 2019, S. 640.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Saul, MJ, Hegewald, AB, Emmerich, AC, Ossipova, E, Vogel, M, Baumann, I, Kultima, K, Lengqivst, J, Steinhilber, D & Jakobsson, PJ 2019, 'Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes', FRONT PHARMACOL, Jg. 10, S. 640. https://doi.org/10.3389/fphar.2019.00640

APA

Saul, M. J., Hegewald, A. B., Emmerich, A. C., Ossipova, E., Vogel, M., Baumann, I., Kultima, K., Lengqivst, J., Steinhilber, D., & Jakobsson, P. J. (2019). Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes. FRONT PHARMACOL, 10, 640. https://doi.org/10.3389/fphar.2019.00640

Vancouver

Saul MJ, Hegewald AB, Emmerich AC, Ossipova E, Vogel M, Baumann I et al. Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes. FRONT PHARMACOL. 2019;10:640. https://doi.org/10.3389/fphar.2019.00640

Bibtex

@article{ed00fb31c657407bac199574185cfac5,
title = "Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes",
abstract = "MicroRNAs (miRs) are small noncoding RNAs which control the expression of target genes by either translational repression or RNA degradation, known as canonical miR functions. The recent discovery that miR-328 has a noncanonical function and can activate gene expression by antagonizing the activity of heterogeneous ribonuclear protein E2 (hnRNP E2) opens an unexplored and exciting field of gene expression regulation. The global importance of such noncanonical miR function is not yet known. In order to achieve a better understanding of the new miR activity, we performed a compartment specific tandem mass tag (TMT)-based proteomic analysis in differentiated MonoMac6 (MM6) cells, to monitor gene expression variations in response to miR-328 knockdown. We identified a broad spectrum of novel potential miR-328/hnRNP E2 and miR-328 targets involved in regulation of compartment specific cellular processes, such as inflammation or RNA splicing. This study provides first insights of the global significance of noncanonical miR function.",
author = "Saul, {Meike J} and Hegewald, {Anett B} and Emmerich, {Anne C} and Elena Ossipova and Marc Vogel and Isabell Baumann and Kim Kultima and Johan Lengqivst and Dieter Steinhilber and Jakobsson, {Per Johan}",
year = "2019",
doi = "10.3389/fphar.2019.00640",
language = "English",
volume = "10",
pages = "640",
journal = "FRONT PHARMACOL",
issn = "1663-9812",
publisher = "Frontiers Media S. A.",

}

RIS

TY - JOUR

T1 - Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes

AU - Saul, Meike J

AU - Hegewald, Anett B

AU - Emmerich, Anne C

AU - Ossipova, Elena

AU - Vogel, Marc

AU - Baumann, Isabell

AU - Kultima, Kim

AU - Lengqivst, Johan

AU - Steinhilber, Dieter

AU - Jakobsson, Per Johan

PY - 2019

Y1 - 2019

N2 - MicroRNAs (miRs) are small noncoding RNAs which control the expression of target genes by either translational repression or RNA degradation, known as canonical miR functions. The recent discovery that miR-328 has a noncanonical function and can activate gene expression by antagonizing the activity of heterogeneous ribonuclear protein E2 (hnRNP E2) opens an unexplored and exciting field of gene expression regulation. The global importance of such noncanonical miR function is not yet known. In order to achieve a better understanding of the new miR activity, we performed a compartment specific tandem mass tag (TMT)-based proteomic analysis in differentiated MonoMac6 (MM6) cells, to monitor gene expression variations in response to miR-328 knockdown. We identified a broad spectrum of novel potential miR-328/hnRNP E2 and miR-328 targets involved in regulation of compartment specific cellular processes, such as inflammation or RNA splicing. This study provides first insights of the global significance of noncanonical miR function.

AB - MicroRNAs (miRs) are small noncoding RNAs which control the expression of target genes by either translational repression or RNA degradation, known as canonical miR functions. The recent discovery that miR-328 has a noncanonical function and can activate gene expression by antagonizing the activity of heterogeneous ribonuclear protein E2 (hnRNP E2) opens an unexplored and exciting field of gene expression regulation. The global importance of such noncanonical miR function is not yet known. In order to achieve a better understanding of the new miR activity, we performed a compartment specific tandem mass tag (TMT)-based proteomic analysis in differentiated MonoMac6 (MM6) cells, to monitor gene expression variations in response to miR-328 knockdown. We identified a broad spectrum of novel potential miR-328/hnRNP E2 and miR-328 targets involved in regulation of compartment specific cellular processes, such as inflammation or RNA splicing. This study provides first insights of the global significance of noncanonical miR function.

U2 - 10.3389/fphar.2019.00640

DO - 10.3389/fphar.2019.00640

M3 - SCORING: Journal article

C2 - 31231226

VL - 10

SP - 640

JO - FRONT PHARMACOL

JF - FRONT PHARMACOL

SN - 1663-9812

ER -