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Mass spectrometric analysis of protein species of biologics

Standard

Mass spectrometric analysis of protein species of biologics. / Richter, Verena; Kwiatkowski, Marcel; Omidi, Maryam; Omidi, Azam; Robertson, Wesley; Schlüter, Hartmut.

in: Pharmaceutical Bioprocessing, Jahrgang 1, Nr. 4, 2013, S. 381-404.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › Rezension › Forschung

Harvard

Richter, V, Kwiatkowski, M, Omidi, M, Omidi, A, Robertson, W & Schlüter, H 2013, 'Mass spectrometric analysis of protein species of biologics', Pharmaceutical Bioprocessing, Jg. 1, Nr. 4, S. 381-404.

APA

Richter, V., Kwiatkowski, M., Omidi, M., Omidi, A., Robertson, W., & Schlüter, H. (2013). Mass spectrometric analysis of protein species of biologics. Pharmaceutical Bioprocessing, 1(4), 381-404.

Vancouver

Richter V, Kwiatkowski M, Omidi M, Omidi A, Robertson W, Schlüter H. Mass spectrometric analysis of protein species of biologics. Pharmaceutical Bioprocessing. 2013; 1(4):381-404.

Bibtex

@article{c724d85abaed4e7d9c57ff082637c014,
title = "Mass spectrometric analysis of protein species of biologics",
abstract = "The global market for therapeutic proteins has been growing fast in the past decade. In comparison to conventional small-molecule drugs therapeutic proteins have a very complex chemical composition. These proteins are typically comprised of dozens to hundreds of amino acids which often are post-translational modified. Recombinant therapeutic proteins are produced by living organisms resulting in a heterogeneous mixture of recombinant protein species (isoforms, proteoforms). The pharmaceutical function and biological and toxicological properties of biologics and follow-on biosimilars are critically related to their exact chemical composition. Therefore, analytical tools capable of identifying and quantifying therapeutic protein species and their accompanying species, often differing in a few chemical moieties only, are necessary to guarantee efficacy and safety of therapeutic proteins. In this review we focus on the application of mass spectrometry for analyzing the exact chemical composition of therapeutic protein species, highlighting especially top-down mass spectrometry.",
author = "Verena Richter and Marcel Kwiatkowski and Maryam Omidi and Azam Omidi and Wesley Robertson and Hartmut Schl{\"u}ter",
year = "2013",
language = "English",
volume = " 1",
pages = "381--404",
journal = "Pharmaceut Bioproc",
issn = "2048-9145",
publisher = "futurescience",
number = "4",

}

RIS

TY - JOUR

T1 - Mass spectrometric analysis of protein species of biologics

AU - Richter, Verena

AU - Kwiatkowski, Marcel

AU - Omidi, Maryam

AU - Omidi, Azam

AU - Robertson, Wesley

AU - Schlüter, Hartmut

PY - 2013

Y1 - 2013

N2 - The global market for therapeutic proteins has been growing fast in the past decade. In comparison to conventional small-molecule drugs therapeutic proteins have a very complex chemical composition. These proteins are typically comprised of dozens to hundreds of amino acids which often are post-translational modified. Recombinant therapeutic proteins are produced by living organisms resulting in a heterogeneous mixture of recombinant protein species (isoforms, proteoforms). The pharmaceutical function and biological and toxicological properties of biologics and follow-on biosimilars are critically related to their exact chemical composition. Therefore, analytical tools capable of identifying and quantifying therapeutic protein species and their accompanying species, often differing in a few chemical moieties only, are necessary to guarantee efficacy and safety of therapeutic proteins. In this review we focus on the application of mass spectrometry for analyzing the exact chemical composition of therapeutic protein species, highlighting especially top-down mass spectrometry.

AB - The global market for therapeutic proteins has been growing fast in the past decade. In comparison to conventional small-molecule drugs therapeutic proteins have a very complex chemical composition. These proteins are typically comprised of dozens to hundreds of amino acids which often are post-translational modified. Recombinant therapeutic proteins are produced by living organisms resulting in a heterogeneous mixture of recombinant protein species (isoforms, proteoforms). The pharmaceutical function and biological and toxicological properties of biologics and follow-on biosimilars are critically related to their exact chemical composition. Therefore, analytical tools capable of identifying and quantifying therapeutic protein species and their accompanying species, often differing in a few chemical moieties only, are necessary to guarantee efficacy and safety of therapeutic proteins. In this review we focus on the application of mass spectrometry for analyzing the exact chemical composition of therapeutic protein species, highlighting especially top-down mass spectrometry.

M3 - Critical review

VL - 1

SP - 381

EP - 404

JO - Pharmaceut Bioproc

JF - Pharmaceut Bioproc

SN - 2048-9145

IS - 4

ER -