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Management of Tamm-Horsfall Protein for Reliable Urinary Analytics

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Management of Tamm-Horsfall Protein for Reliable Urinary Analytics. / Xu, Xiaomeng; Barreiro, Karina; Musante, Luca; Kretz, Oliver; Lin, Hanfei; Zou, Hequn; Huber, Tobias B; Holthofer, Harry.

in: PROTEOM CLIN APPL, Jahrgang 13, Nr. 6, 11.2019, S. e1900018.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Xu, X, Barreiro, K, Musante, L, Kretz, O, Lin, H, Zou, H, Huber, TB & Holthofer, H 2019, 'Management of Tamm-Horsfall Protein for Reliable Urinary Analytics', PROTEOM CLIN APPL, Jg. 13, Nr. 6, S. e1900018. https://doi.org/10.1002/prca.201900018

APA

Xu, X., Barreiro, K., Musante, L., Kretz, O., Lin, H., Zou, H., Huber, T. B., & Holthofer, H. (2019). Management of Tamm-Horsfall Protein for Reliable Urinary Analytics. PROTEOM CLIN APPL, 13(6), e1900018. https://doi.org/10.1002/prca.201900018

Vancouver

Xu X, Barreiro K, Musante L, Kretz O, Lin H, Zou H et al. Management of Tamm-Horsfall Protein for Reliable Urinary Analytics. PROTEOM CLIN APPL. 2019 Nov;13(6):e1900018. https://doi.org/10.1002/prca.201900018

Bibtex

@article{6b23c9556c984901a5e032f1b5a13022,
title = "Management of Tamm-Horsfall Protein for Reliable Urinary Analytics",
abstract = "PURPOSE: Urinary extracellular vesicles (uEVs) are a novel source of biomarkers. However, urinary Tamm-Horsfall Protein (THP; uromodulin) interferes with all vesicle isolation attempts, precipitates with normal urinary proteins, thus, representing an unwanted {"}contaminant{"} in urinary assays. Thus, the aim is to develop a simple method to manage THP efficiently.EXPERIMENTAL DESIGN: The uEVs are isolated by hydrostatic filtration dialysis (HFD) and treated with a defined solution of urea to optimize release of uEVs from sample. Presence of uEVs is confirmed by transmission electron microscopy, Western blotting, and proteomic profiling in MS.RESULTS: Using HFD with urea treatment for uEV isolation reduces sample complexity to a great extent. The novel simplified uEV isolation protocol allows comprehensive vesicle proteomics analysis and should be part of any urine analytics to release all sample constituents from THP trap.CONCLUSIONS AND CLINICAL RELEVANCE: The method brings a quick and easy protocol for THP management during uEV isolation, providing major benefits for comprehensive sample analytics.",
author = "Xiaomeng Xu and Karina Barreiro and Luca Musante and Oliver Kretz and Hanfei Lin and Hequn Zou and Huber, {Tobias B} and Harry Holthofer",
note = "{\textcopyright} 2019 The Authors. Proteomics - Clinical Application published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2019",
month = nov,
doi = "10.1002/prca.201900018",
language = "English",
volume = "13",
pages = "e1900018",
journal = "PROTEOM CLIN APPL",
issn = "1862-8346",
publisher = "Wiley-VCH Verlag GmbH",
number = "6",

}

RIS

TY - JOUR

T1 - Management of Tamm-Horsfall Protein for Reliable Urinary Analytics

AU - Xu, Xiaomeng

AU - Barreiro, Karina

AU - Musante, Luca

AU - Kretz, Oliver

AU - Lin, Hanfei

AU - Zou, Hequn

AU - Huber, Tobias B

AU - Holthofer, Harry

N1 - © 2019 The Authors. Proteomics - Clinical Application published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2019/11

Y1 - 2019/11

N2 - PURPOSE: Urinary extracellular vesicles (uEVs) are a novel source of biomarkers. However, urinary Tamm-Horsfall Protein (THP; uromodulin) interferes with all vesicle isolation attempts, precipitates with normal urinary proteins, thus, representing an unwanted "contaminant" in urinary assays. Thus, the aim is to develop a simple method to manage THP efficiently.EXPERIMENTAL DESIGN: The uEVs are isolated by hydrostatic filtration dialysis (HFD) and treated with a defined solution of urea to optimize release of uEVs from sample. Presence of uEVs is confirmed by transmission electron microscopy, Western blotting, and proteomic profiling in MS.RESULTS: Using HFD with urea treatment for uEV isolation reduces sample complexity to a great extent. The novel simplified uEV isolation protocol allows comprehensive vesicle proteomics analysis and should be part of any urine analytics to release all sample constituents from THP trap.CONCLUSIONS AND CLINICAL RELEVANCE: The method brings a quick and easy protocol for THP management during uEV isolation, providing major benefits for comprehensive sample analytics.

AB - PURPOSE: Urinary extracellular vesicles (uEVs) are a novel source of biomarkers. However, urinary Tamm-Horsfall Protein (THP; uromodulin) interferes with all vesicle isolation attempts, precipitates with normal urinary proteins, thus, representing an unwanted "contaminant" in urinary assays. Thus, the aim is to develop a simple method to manage THP efficiently.EXPERIMENTAL DESIGN: The uEVs are isolated by hydrostatic filtration dialysis (HFD) and treated with a defined solution of urea to optimize release of uEVs from sample. Presence of uEVs is confirmed by transmission electron microscopy, Western blotting, and proteomic profiling in MS.RESULTS: Using HFD with urea treatment for uEV isolation reduces sample complexity to a great extent. The novel simplified uEV isolation protocol allows comprehensive vesicle proteomics analysis and should be part of any urine analytics to release all sample constituents from THP trap.CONCLUSIONS AND CLINICAL RELEVANCE: The method brings a quick and easy protocol for THP management during uEV isolation, providing major benefits for comprehensive sample analytics.

U2 - 10.1002/prca.201900018

DO - 10.1002/prca.201900018

M3 - SCORING: Journal article

C2 - 31424164

VL - 13

SP - e1900018

JO - PROTEOM CLIN APPL

JF - PROTEOM CLIN APPL

SN - 1862-8346

IS - 6

ER -