Makorin ring zinc finger protein 1 (MKRN1), a novel poly(A)-binding protein-interacting protein, stimulates translation in nerve cells.
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Makorin ring zinc finger protein 1 (MKRN1), a novel poly(A)-binding protein-interacting protein, stimulates translation in nerve cells. / Miroci, Hatmone; Schob, Claudia; Kindler, Stefan; Ölschläger-Schütt, Janin; Fehr, Susanne; Jungenitz, Tassilo; Schwarzacher, Stephan W; Bagni, Claudia; Mohr, Evita.
in: J BIOL CHEM, Jahrgang 287, Nr. 2, 2, 2012, S. 1322-1334.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Makorin ring zinc finger protein 1 (MKRN1), a novel poly(A)-binding protein-interacting protein, stimulates translation in nerve cells.
AU - Miroci, Hatmone
AU - Schob, Claudia
AU - Kindler, Stefan
AU - Ölschläger-Schütt, Janin
AU - Fehr, Susanne
AU - Jungenitz, Tassilo
AU - Schwarzacher, Stephan W
AU - Bagni, Claudia
AU - Mohr, Evita
PY - 2012
Y1 - 2012
N2 - The poly(A)-binding protein (PABP), a key component of different ribonucleoprotein complexes, plays a crucial role in the control of mRNA translation rates, stability, and subcellular targeting. In this study we identify RING zinc finger protein Makorin 1 (MKRN1), a bona fide RNA-binding protein, as a binding partner of PABP that interacts with PABP in an RNA-independent manner. In rat brain, a so far uncharacterized short MKRN1 isoform, MKRN1-short, predominates and is detected in forebrain nerve cells. In neuronal dendrites, MKRN1-short co-localizes with PABP in granule-like structures, which are morphological correlates of sites of mRNA metabolism. Moreover, in primary rat neurons MKRN1-short associates with dendritically localized mRNAs. When tethered to a reporter mRNA, MKRN1-short significantly enhances reporter protein synthesis. Furthermore, after induction of synaptic plasticity via electrical stimulation of the perforant path in vivo, MKRN1-short specifically accumulates in the activated dendritic lamina, the middle molecular layer of the hippocampal dentate gyrus. Collectively, these data indicate that in mammalian neurons MKRN1-short interacts with PABP to locally control the translation of dendritic mRNAs at synapses.
AB - The poly(A)-binding protein (PABP), a key component of different ribonucleoprotein complexes, plays a crucial role in the control of mRNA translation rates, stability, and subcellular targeting. In this study we identify RING zinc finger protein Makorin 1 (MKRN1), a bona fide RNA-binding protein, as a binding partner of PABP that interacts with PABP in an RNA-independent manner. In rat brain, a so far uncharacterized short MKRN1 isoform, MKRN1-short, predominates and is detected in forebrain nerve cells. In neuronal dendrites, MKRN1-short co-localizes with PABP in granule-like structures, which are morphological correlates of sites of mRNA metabolism. Moreover, in primary rat neurons MKRN1-short associates with dendritically localized mRNAs. When tethered to a reporter mRNA, MKRN1-short significantly enhances reporter protein synthesis. Furthermore, after induction of synaptic plasticity via electrical stimulation of the perforant path in vivo, MKRN1-short specifically accumulates in the activated dendritic lamina, the middle molecular layer of the hippocampal dentate gyrus. Collectively, these data indicate that in mammalian neurons MKRN1-short interacts with PABP to locally control the translation of dendritic mRNAs at synapses.
KW - Animals
KW - Male
KW - Rats
KW - Rats, Sprague-Dawley
KW - Dendrites/genetics/metabolism
KW - Dentate Gyrus/cytology/metabolism
KW - Nerve Tissue Proteins/genetics/metabolism
KW - Neuronal Plasticity/physiology
KW - Poly(A)-Binding Proteins/genetics/metabolism
KW - Protein Biosynthesis/physiology
KW - Protein Isoforms/genetics/metabolism
KW - RNA, Messenger/genetics/metabolism
KW - Synapses/genetics/metabolism
KW - Animals
KW - Male
KW - Rats
KW - Rats, Sprague-Dawley
KW - Dendrites/genetics/metabolism
KW - Dentate Gyrus/cytology/metabolism
KW - Nerve Tissue Proteins/genetics/metabolism
KW - Neuronal Plasticity/physiology
KW - Poly(A)-Binding Proteins/genetics/metabolism
KW - Protein Biosynthesis/physiology
KW - Protein Isoforms/genetics/metabolism
KW - RNA, Messenger/genetics/metabolism
KW - Synapses/genetics/metabolism
M3 - SCORING: Journal article
VL - 287
SP - 1322
EP - 1334
JO - J BIOL CHEM
JF - J BIOL CHEM
SN - 0021-9258
IS - 2
M1 - 2
ER -