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Magnetic resonance T1 relaxation time of venous thrombus is determined by iron processing and predicts susceptibility to lysis

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Magnetic resonance T1 relaxation time of venous thrombus is determined by iron processing and predicts susceptibility to lysis. / Saha, Prakash; Andia, Marcelo E; Modarai, Bijan; Blume, Ulrike; Humphries, Julia; Patel, Ashish S; Phinikaridou, Alkystis; Evans, Colin E; Mattock, Katherine; Grover, Steven P; Ahmad, Anwar; Lyons, Oliver T; Attia, Rizwan Q; Renné, Thomas; Premaratne, Sobath; Wiethoff, Andrea J; Botnar, René M; Schaeffter, Tobias; Waltham, Matthew; Smith, Alberto.

in: CIRCULATION, Jahrgang 128, Nr. 7, 13.08.2013, S. 729-36.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Saha, P, Andia, ME, Modarai, B, Blume, U, Humphries, J, Patel, AS, Phinikaridou, A, Evans, CE, Mattock, K, Grover, SP, Ahmad, A, Lyons, OT, Attia, RQ, Renné, T, Premaratne, S, Wiethoff, AJ, Botnar, RM, Schaeffter, T, Waltham, M & Smith, A 2013, 'Magnetic resonance T1 relaxation time of venous thrombus is determined by iron processing and predicts susceptibility to lysis', CIRCULATION, Jg. 128, Nr. 7, S. 729-36. https://doi.org/10.1161/CIRCULATIONAHA.113.001371

APA

Saha, P., Andia, M. E., Modarai, B., Blume, U., Humphries, J., Patel, A. S., Phinikaridou, A., Evans, C. E., Mattock, K., Grover, S. P., Ahmad, A., Lyons, O. T., Attia, R. Q., Renné, T., Premaratne, S., Wiethoff, A. J., Botnar, R. M., Schaeffter, T., Waltham, M., & Smith, A. (2013). Magnetic resonance T1 relaxation time of venous thrombus is determined by iron processing and predicts susceptibility to lysis. CIRCULATION, 128(7), 729-36. https://doi.org/10.1161/CIRCULATIONAHA.113.001371

Vancouver

Saha P, Andia ME, Modarai B, Blume U, Humphries J, Patel AS et al. Magnetic resonance T1 relaxation time of venous thrombus is determined by iron processing and predicts susceptibility to lysis. CIRCULATION. 2013 Aug 13;128(7):729-36. https://doi.org/10.1161/CIRCULATIONAHA.113.001371

Bibtex

@article{343180b39c1640118baa5e1ea80fcc58,
title = "Magnetic resonance T1 relaxation time of venous thrombus is determined by iron processing and predicts susceptibility to lysis",
abstract = "BACKGROUND: The magnetic resonance longitudinal relaxation time (T1) changes with thrombus age in humans. In this study, we investigate the possible mechanisms that give rise to the T1 signal in venous thrombi and whether changes in T1 relaxation time are informative of the susceptibility to lysis.METHODS AND RESULTS: Venous thrombosis was induced in the vena cava of BALB/C mice, and temporal changes in T1 relaxation time correlated with thrombus composition. The mean T1 relaxation time of thrombus was shortest at 7 days following thrombus induction and returned to that of blood as the thrombus resolved. T1 relaxation time was related to thrombus methemoglobin formation and further processing. Studies in inducible nitric oxide synthase (iNOS(-/-))-deficient mice revealed that inducible nitric oxide synthase mediates oxidation of erythrocyte lysis-derived iron to paramagnetic Fe3+, which causes thrombus T1 relaxation time shortening. Studies using chemokine receptor-2-deficient mice (Ccr2(-/-)) revealed that the return of the T1 signal to that of blood is regulated by removal of Fe3+ by macrophages that accumulate in the thrombus during its resolution. Quantification of T1 relaxation time was a good predictor of successful thrombolysis with a cutoff point of <747 ms having a sensitivity and specificity to predict successful lysis of 83% and 94%, respectively.CONCLUSIONS: The source of the T1 signal in the thrombus results from the oxidation of iron (released from the lysis of trapped erythrocytes in the thrombus) to its paramagnetic Fe3+ form. Quantification of T1 relaxation time appears to be a good predictor of the success of thrombolysis.",
keywords = "Animals, Endothelium, Vascular, Erythrocytes, Fibrinolysis, Humans, Iron, Ligation, Macrophages, Magnetic Resonance Imaging, Male, Mass Spectrometry, Methemoglobin, Mice, Mice, Inbred BALB C, Mice, Knockout, Nitric Oxide Synthase Type II, Oxidation-Reduction, Receptors, CCR2, Time Factors, Vena Cava, Inferior, Venous Thrombosis",
author = "Prakash Saha and Andia, {Marcelo E} and Bijan Modarai and Ulrike Blume and Julia Humphries and Patel, {Ashish S} and Alkystis Phinikaridou and Evans, {Colin E} and Katherine Mattock and Grover, {Steven P} and Anwar Ahmad and Lyons, {Oliver T} and Attia, {Rizwan Q} and Thomas Renn{\'e} and Sobath Premaratne and Wiethoff, {Andrea J} and Botnar, {Ren{\'e} M} and Tobias Schaeffter and Matthew Waltham and Alberto Smith",
year = "2013",
month = aug,
day = "13",
doi = "10.1161/CIRCULATIONAHA.113.001371",
language = "English",
volume = "128",
pages = "729--36",
journal = "CIRCULATION",
issn = "0009-7322",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

RIS

TY - JOUR

T1 - Magnetic resonance T1 relaxation time of venous thrombus is determined by iron processing and predicts susceptibility to lysis

AU - Saha, Prakash

AU - Andia, Marcelo E

AU - Modarai, Bijan

AU - Blume, Ulrike

AU - Humphries, Julia

AU - Patel, Ashish S

AU - Phinikaridou, Alkystis

AU - Evans, Colin E

AU - Mattock, Katherine

AU - Grover, Steven P

AU - Ahmad, Anwar

AU - Lyons, Oliver T

AU - Attia, Rizwan Q

AU - Renné, Thomas

AU - Premaratne, Sobath

AU - Wiethoff, Andrea J

AU - Botnar, René M

AU - Schaeffter, Tobias

AU - Waltham, Matthew

AU - Smith, Alberto

PY - 2013/8/13

Y1 - 2013/8/13

N2 - BACKGROUND: The magnetic resonance longitudinal relaxation time (T1) changes with thrombus age in humans. In this study, we investigate the possible mechanisms that give rise to the T1 signal in venous thrombi and whether changes in T1 relaxation time are informative of the susceptibility to lysis.METHODS AND RESULTS: Venous thrombosis was induced in the vena cava of BALB/C mice, and temporal changes in T1 relaxation time correlated with thrombus composition. The mean T1 relaxation time of thrombus was shortest at 7 days following thrombus induction and returned to that of blood as the thrombus resolved. T1 relaxation time was related to thrombus methemoglobin formation and further processing. Studies in inducible nitric oxide synthase (iNOS(-/-))-deficient mice revealed that inducible nitric oxide synthase mediates oxidation of erythrocyte lysis-derived iron to paramagnetic Fe3+, which causes thrombus T1 relaxation time shortening. Studies using chemokine receptor-2-deficient mice (Ccr2(-/-)) revealed that the return of the T1 signal to that of blood is regulated by removal of Fe3+ by macrophages that accumulate in the thrombus during its resolution. Quantification of T1 relaxation time was a good predictor of successful thrombolysis with a cutoff point of <747 ms having a sensitivity and specificity to predict successful lysis of 83% and 94%, respectively.CONCLUSIONS: The source of the T1 signal in the thrombus results from the oxidation of iron (released from the lysis of trapped erythrocytes in the thrombus) to its paramagnetic Fe3+ form. Quantification of T1 relaxation time appears to be a good predictor of the success of thrombolysis.

AB - BACKGROUND: The magnetic resonance longitudinal relaxation time (T1) changes with thrombus age in humans. In this study, we investigate the possible mechanisms that give rise to the T1 signal in venous thrombi and whether changes in T1 relaxation time are informative of the susceptibility to lysis.METHODS AND RESULTS: Venous thrombosis was induced in the vena cava of BALB/C mice, and temporal changes in T1 relaxation time correlated with thrombus composition. The mean T1 relaxation time of thrombus was shortest at 7 days following thrombus induction and returned to that of blood as the thrombus resolved. T1 relaxation time was related to thrombus methemoglobin formation and further processing. Studies in inducible nitric oxide synthase (iNOS(-/-))-deficient mice revealed that inducible nitric oxide synthase mediates oxidation of erythrocyte lysis-derived iron to paramagnetic Fe3+, which causes thrombus T1 relaxation time shortening. Studies using chemokine receptor-2-deficient mice (Ccr2(-/-)) revealed that the return of the T1 signal to that of blood is regulated by removal of Fe3+ by macrophages that accumulate in the thrombus during its resolution. Quantification of T1 relaxation time was a good predictor of successful thrombolysis with a cutoff point of <747 ms having a sensitivity and specificity to predict successful lysis of 83% and 94%, respectively.CONCLUSIONS: The source of the T1 signal in the thrombus results from the oxidation of iron (released from the lysis of trapped erythrocytes in the thrombus) to its paramagnetic Fe3+ form. Quantification of T1 relaxation time appears to be a good predictor of the success of thrombolysis.

KW - Animals

KW - Endothelium, Vascular

KW - Erythrocytes

KW - Fibrinolysis

KW - Humans

KW - Iron

KW - Ligation

KW - Macrophages

KW - Magnetic Resonance Imaging

KW - Male

KW - Mass Spectrometry

KW - Methemoglobin

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Knockout

KW - Nitric Oxide Synthase Type II

KW - Oxidation-Reduction

KW - Receptors, CCR2

KW - Time Factors

KW - Vena Cava, Inferior

KW - Venous Thrombosis

U2 - 10.1161/CIRCULATIONAHA.113.001371

DO - 10.1161/CIRCULATIONAHA.113.001371

M3 - SCORING: Journal article

C2 - 23820077

VL - 128

SP - 729

EP - 736

JO - CIRCULATION

JF - CIRCULATION

SN - 0009-7322

IS - 7

ER -