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Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes

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Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes. / Horváth, András; Lemoine, Marc D; Löser, Alexandra; Mannhardt, Ingra; Flenner, Frederik; Uzun, Ahmet Umur; Neuber, Christiane; Breckwoldt, Kaja; Hansen, Arne; Girdauskas, Evaldas; Reichenspurner, Hermann; Willems, Stephan; Jost, Norbert; Wettwer, Erich; Eschenhagen, Thomas; Christ, Torsten.

in: STEM CELL REP, Jahrgang 10, Nr. 3, 13.03.2018, S. 822-833.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Horváth, A, Lemoine, MD, Löser, A, Mannhardt, I, Flenner, F, Uzun, AU, Neuber, C, Breckwoldt, K, Hansen, A, Girdauskas, E, Reichenspurner, H, Willems, S, Jost, N, Wettwer, E, Eschenhagen, T & Christ, T 2018, 'Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes', STEM CELL REP, Jg. 10, Nr. 3, S. 822-833. https://doi.org/10.1016/j.stemcr.2018.01.012

APA

Horváth, A., Lemoine, M. D., Löser, A., Mannhardt, I., Flenner, F., Uzun, A. U., Neuber, C., Breckwoldt, K., Hansen, A., Girdauskas, E., Reichenspurner, H., Willems, S., Jost, N., Wettwer, E., Eschenhagen, T., & Christ, T. (2018). Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes. STEM CELL REP, 10(3), 822-833. https://doi.org/10.1016/j.stemcr.2018.01.012

Vancouver

Horváth A, Lemoine MD, Löser A, Mannhardt I, Flenner F, Uzun AU et al. Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes. STEM CELL REP. 2018 Mär 13;10(3):822-833. https://doi.org/10.1016/j.stemcr.2018.01.012

Bibtex

@article{9edbb3a65eae42ccb4b00debb9cd8647,
title = "Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes",
abstract = "Human induced pluripotent stem cell (hiPSC) cardiomyocytes (CMs) show less negative resting membrane potential (RMP), which is attributed to small inward rectifier currents (IK1). Here, IK1 was measured in hiPSC-CMs (proprietary and commercial cell line) cultured as monolayer (ML) or 3D engineered heart tissue (EHT) and, for direct comparison, in CMs from human right atrial (RA) and left ventricular (LV) tissue. RMP was measured in isolated cells and intact tissues. IK1 density in ML- and EHT-CMs from the proprietary line was similar to LV and RA, respectively. IK1 density in EHT-CMs from the commercial line was 2-fold smaller than in the proprietary line. RMP in EHT of both lines was similar to RA and LV. Repolarization fraction and IK,ACh response discriminated best between RA and LV and indicated predominantly ventricular phenotype in hiPSC-CMs/EHT. The data indicate that IK1 is not necessarily low in hiPSC-CMs, and technical issues may underlie low RMP in hiPSC-CMs.",
keywords = "Journal Article",
author = "Andr{\'a}s Horv{\'a}th and Lemoine, {Marc D} and Alexandra L{\"o}ser and Ingra Mannhardt and Frederik Flenner and Uzun, {Ahmet Umur} and Christiane Neuber and Kaja Breckwoldt and Arne Hansen and Evaldas Girdauskas and Hermann Reichenspurner and Stephan Willems and Norbert Jost and Erich Wettwer and Thomas Eschenhagen and Torsten Christ",
note = "Copyright {\textcopyright} 2018 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2018",
month = mar,
day = "13",
doi = "10.1016/j.stemcr.2018.01.012",
language = "English",
volume = "10",
pages = "822--833",
journal = "STEM CELL REP",
issn = "2213-6711",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes

AU - Horváth, András

AU - Lemoine, Marc D

AU - Löser, Alexandra

AU - Mannhardt, Ingra

AU - Flenner, Frederik

AU - Uzun, Ahmet Umur

AU - Neuber, Christiane

AU - Breckwoldt, Kaja

AU - Hansen, Arne

AU - Girdauskas, Evaldas

AU - Reichenspurner, Hermann

AU - Willems, Stephan

AU - Jost, Norbert

AU - Wettwer, Erich

AU - Eschenhagen, Thomas

AU - Christ, Torsten

N1 - Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2018/3/13

Y1 - 2018/3/13

N2 - Human induced pluripotent stem cell (hiPSC) cardiomyocytes (CMs) show less negative resting membrane potential (RMP), which is attributed to small inward rectifier currents (IK1). Here, IK1 was measured in hiPSC-CMs (proprietary and commercial cell line) cultured as monolayer (ML) or 3D engineered heart tissue (EHT) and, for direct comparison, in CMs from human right atrial (RA) and left ventricular (LV) tissue. RMP was measured in isolated cells and intact tissues. IK1 density in ML- and EHT-CMs from the proprietary line was similar to LV and RA, respectively. IK1 density in EHT-CMs from the commercial line was 2-fold smaller than in the proprietary line. RMP in EHT of both lines was similar to RA and LV. Repolarization fraction and IK,ACh response discriminated best between RA and LV and indicated predominantly ventricular phenotype in hiPSC-CMs/EHT. The data indicate that IK1 is not necessarily low in hiPSC-CMs, and technical issues may underlie low RMP in hiPSC-CMs.

AB - Human induced pluripotent stem cell (hiPSC) cardiomyocytes (CMs) show less negative resting membrane potential (RMP), which is attributed to small inward rectifier currents (IK1). Here, IK1 was measured in hiPSC-CMs (proprietary and commercial cell line) cultured as monolayer (ML) or 3D engineered heart tissue (EHT) and, for direct comparison, in CMs from human right atrial (RA) and left ventricular (LV) tissue. RMP was measured in isolated cells and intact tissues. IK1 density in ML- and EHT-CMs from the proprietary line was similar to LV and RA, respectively. IK1 density in EHT-CMs from the commercial line was 2-fold smaller than in the proprietary line. RMP in EHT of both lines was similar to RA and LV. Repolarization fraction and IK,ACh response discriminated best between RA and LV and indicated predominantly ventricular phenotype in hiPSC-CMs/EHT. The data indicate that IK1 is not necessarily low in hiPSC-CMs, and technical issues may underlie low RMP in hiPSC-CMs.

KW - Journal Article

U2 - 10.1016/j.stemcr.2018.01.012

DO - 10.1016/j.stemcr.2018.01.012

M3 - SCORING: Journal article

C2 - 29429959

VL - 10

SP - 822

EP - 833

JO - STEM CELL REP

JF - STEM CELL REP

SN - 2213-6711

IS - 3

ER -