PortalPublikationenLoss of retroviral gene expression in bone marrow reconstitu...

Loss of retroviral gene expression in bone marrow reconstituted mice correlates with down-regulation of gene expression in long-term culture initiating cells

Standard

Loss of retroviral gene expression in bone marrow reconstituted mice correlates with down-regulation of gene expression in long-term culture initiating cells. / Lange, C; Blankenstein, T.

in: GENE THER, Jahrgang 4, Nr. 4, 01.04.1997, S. 303-8.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Lange, C & Blankenstein, T 1997, 'Loss of retroviral gene expression in bone marrow reconstituted mice correlates with down-regulation of gene expression in long-term culture initiating cells', GENE THER, Jg. 4, Nr. 4, S. 303-8. https://doi.org/10.1038/sj.gt.3300395

APA

Lange, C., & Blankenstein, T. (1997). Loss of retroviral gene expression in bone marrow reconstituted mice correlates with down-regulation of gene expression in long-term culture initiating cells. GENE THER, 4(4), 303-8. https://doi.org/10.1038/sj.gt.3300395

Vancouver

Lange C, Blankenstein T. Loss of retroviral gene expression in bone marrow reconstituted mice correlates with down-regulation of gene expression in long-term culture initiating cells. GENE THER. 1997 Apr 1;4(4):303-8. https://doi.org/10.1038/sj.gt.3300395

Bibtex

@article{1b84f0db2c01462b9e6833ac49fb8cad,
title = "Loss of retroviral gene expression in bone marrow reconstituted mice correlates with down-regulation of gene expression in long-term culture initiating cells",
abstract = "The question of whether a retroviral vector is expressed in vivo after infection of hematopoietic stem cells remains unpredictable. In this study, we show that a switch off of retroviral sequences can be tested in vitro using the long-term bone marrow culture system. The time kinetics of the down-regulation of retroviral expression in vitro and in vivo have been demonstrated to be similar. The correlation of switch off events in both systems allows for evaluation of retroviral expression in vitro provided the silencing is caused by viral sequences. This approach could be useful for testing vectors for gene therapeutical applications of the hematopoietic system.",
keywords = "Animals, Cells, Cultured, Female, Gene Expression Regulation, Viral, Genes, Viral, Hematopoietic Stem Cells, Mice, Mice, Inbred C57BL, Retroviridae, Time Factors, Transfection",
author = "C Lange and T Blankenstein",
year = "1997",
month = apr,
day = "1",
doi = "10.1038/sj.gt.3300395",
language = "English",
volume = "4",
pages = "303--8",
journal = "GENE THER",
issn = "0969-7128",
publisher = "NATURE PUBLISHING GROUP",
number = "4",

}

RIS

TY - JOUR

T1 - Loss of retroviral gene expression in bone marrow reconstituted mice correlates with down-regulation of gene expression in long-term culture initiating cells

AU - Lange, C

AU - Blankenstein, T

PY - 1997/4/1

Y1 - 1997/4/1

N2 - The question of whether a retroviral vector is expressed in vivo after infection of hematopoietic stem cells remains unpredictable. In this study, we show that a switch off of retroviral sequences can be tested in vitro using the long-term bone marrow culture system. The time kinetics of the down-regulation of retroviral expression in vitro and in vivo have been demonstrated to be similar. The correlation of switch off events in both systems allows for evaluation of retroviral expression in vitro provided the silencing is caused by viral sequences. This approach could be useful for testing vectors for gene therapeutical applications of the hematopoietic system.

AB - The question of whether a retroviral vector is expressed in vivo after infection of hematopoietic stem cells remains unpredictable. In this study, we show that a switch off of retroviral sequences can be tested in vitro using the long-term bone marrow culture system. The time kinetics of the down-regulation of retroviral expression in vitro and in vivo have been demonstrated to be similar. The correlation of switch off events in both systems allows for evaluation of retroviral expression in vitro provided the silencing is caused by viral sequences. This approach could be useful for testing vectors for gene therapeutical applications of the hematopoietic system.

KW - Animals

KW - Cells, Cultured

KW - Female

KW - Gene Expression Regulation, Viral

KW - Genes, Viral

KW - Hematopoietic Stem Cells

KW - Mice

KW - Mice, Inbred C57BL

KW - Retroviridae

KW - Time Factors

KW - Transfection

U2 - 10.1038/sj.gt.3300395

DO - 10.1038/sj.gt.3300395

M3 - SCORING: Journal article

C2 - 9176515

VL - 4

SP - 303

EP - 308

JO - GENE THER

JF - GENE THER

SN - 0969-7128

IS - 4

ER -