Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects.
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Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects. / Grüner, Florian; Blumendorf, Florian; Schmutzler, Oliver; Staufer, Theresa; Bradbury, Michelle; Wiesner, Ulrich; Rosentreter, Tanja; Loers, Gabriele; Lutz, David; Richter, Bernadette; Fischer, Markus; Schulz, Florian; Steiner, Swantje; Warmer, Martin; Burkhardt, Anja; Meents, Alke; Kupinski, Matthew; Hoeschen, Christoph.
in: SCI REP-UK, Jahrgang 8, Nr. 1, 08.11.2018, S. 16561.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects.
AU - Grüner, Florian
AU - Blumendorf, Florian
AU - Schmutzler, Oliver
AU - Staufer, Theresa
AU - Bradbury, Michelle
AU - Wiesner, Ulrich
AU - Rosentreter, Tanja
AU - Loers, Gabriele
AU - Lutz, David
AU - Richter, Bernadette
AU - Fischer, Markus
AU - Schulz, Florian
AU - Steiner, Swantje
AU - Warmer, Martin
AU - Burkhardt, Anja
AU - Meents, Alke
AU - Kupinski, Matthew
AU - Hoeschen, Christoph
PY - 2018/11/8
Y1 - 2018/11/8
N2 - Accurate in vivo localisation of minimal amounts of functionalised gold-nanoparticles, enabling e.g. early-tumour diagnostics and pharmacokinetic tracking studies, requires a precision imaging system offering very high sensitivity, temporal and spatial resolution, large depth penetration, and arbitrarily long serial measurements. X-ray fluorescence imaging could offer such capabilities; however, its utilisation for human-sized scales is hampered by a high intrinsic background level. Here we measure and model this anisotropic background and present a spatial filtering scheme for background reduction enabling the localisation of nanoparticle-amounts as reported from small-animal tumour models. As a basic application study towards precision pharmacokinetics, we demonstrate specific localisation to sites of disease by adapting gold-nanoparticles with small targeting ligands in murine spinal cord injury models, at record sensitivity levels using sub-mm resolution. Both studies contribute to the future use of molecularly-targeted gold-nanoparticles as next-generation clinical diagnostic and pharmacokinetic tools.
AB - Accurate in vivo localisation of minimal amounts of functionalised gold-nanoparticles, enabling e.g. early-tumour diagnostics and pharmacokinetic tracking studies, requires a precision imaging system offering very high sensitivity, temporal and spatial resolution, large depth penetration, and arbitrarily long serial measurements. X-ray fluorescence imaging could offer such capabilities; however, its utilisation for human-sized scales is hampered by a high intrinsic background level. Here we measure and model this anisotropic background and present a spatial filtering scheme for background reduction enabling the localisation of nanoparticle-amounts as reported from small-animal tumour models. As a basic application study towards precision pharmacokinetics, we demonstrate specific localisation to sites of disease by adapting gold-nanoparticles with small targeting ligands in murine spinal cord injury models, at record sensitivity levels using sub-mm resolution. Both studies contribute to the future use of molecularly-targeted gold-nanoparticles as next-generation clinical diagnostic and pharmacokinetic tools.
M3 - SCORING: Journal article
VL - 8
SP - 16561
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
IS - 1
ER -
