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Liver-specific gene expression in cultured human hematopoietic stem cells.

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Liver-specific gene expression in cultured human hematopoietic stem cells. / Fiegel, Henning C; Lioznov, Michael; Cortes-Dericks, Lourdes; Lange, Claudia; Kluth, Dietrich; Fehse, Boris; Zander, Axel R.

in: STEM CELLS, Jahrgang 21, Nr. 1, 1, 2003, S. 98-104.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Fiegel, HC, Lioznov, M, Cortes-Dericks, L, Lange, C, Kluth, D, Fehse, B & Zander, AR 2003, 'Liver-specific gene expression in cultured human hematopoietic stem cells.', STEM CELLS, Jg. 21, Nr. 1, 1, S. 98-104. <http://www.ncbi.nlm.nih.gov/pubmed/12529556?dopt=Citation>

APA

Fiegel, H. C., Lioznov, M., Cortes-Dericks, L., Lange, C., Kluth, D., Fehse, B., & Zander, A. R. (2003). Liver-specific gene expression in cultured human hematopoietic stem cells. STEM CELLS, 21(1), 98-104. [1]. http://www.ncbi.nlm.nih.gov/pubmed/12529556?dopt=Citation

Vancouver

Fiegel HC, Lioznov M, Cortes-Dericks L, Lange C, Kluth D, Fehse B et al. Liver-specific gene expression in cultured human hematopoietic stem cells. STEM CELLS. 2003;21(1):98-104. 1.

Bibtex

@article{9e704939cd8e48b0b7d33c087217b3bb,
title = "Liver-specific gene expression in cultured human hematopoietic stem cells.",
abstract = "Hematopoietic and hepatic stem cells share characteristic markers such as CD34, c-kit, and Thy1. Based on the recent observations that hepatocytes may originate from bone marrow, we investigated the potential of CD34(+) bone marrow cells to differentiate into hepatocytic cells in vitro. CD34(+) and CD34(-) human bone marrow cells were separated by magnetic cell sorting. Cells were cultured on a collagen matrix in a defined medium containing hepatocyte growth factor. Cell count and size were measured by flow cytometry, and reverse transcription polymerase chain reaction was carried out for the liver-specific markers CK-19 and albumin. During cell culture, CD34(+) cells showed an increasing cell number and proliferative activity as assessed by Ki-67 staining. Under the specified culture conditions, CD34(+) cells expressed albumin RNA and CK-19 RNA after 28 days, whereas CD34(-) cells did not show liver-specific gene expression. The results indicate that CD34(+) adult human bone marrow stem cells can differentiate into hepatocytic cells in vitro.",
author = "Fiegel, {Henning C} and Michael Lioznov and Lourdes Cortes-Dericks and Claudia Lange and Dietrich Kluth and Boris Fehse and Zander, {Axel R}",
year = "2003",
language = "Deutsch",
volume = "21",
pages = "98--104",
journal = "STEM CELLS",
issn = "1066-5099",
publisher = "ALPHAMED PRESS",
number = "1",

}

RIS

TY - JOUR

T1 - Liver-specific gene expression in cultured human hematopoietic stem cells.

AU - Fiegel, Henning C

AU - Lioznov, Michael

AU - Cortes-Dericks, Lourdes

AU - Lange, Claudia

AU - Kluth, Dietrich

AU - Fehse, Boris

AU - Zander, Axel R

PY - 2003

Y1 - 2003

N2 - Hematopoietic and hepatic stem cells share characteristic markers such as CD34, c-kit, and Thy1. Based on the recent observations that hepatocytes may originate from bone marrow, we investigated the potential of CD34(+) bone marrow cells to differentiate into hepatocytic cells in vitro. CD34(+) and CD34(-) human bone marrow cells were separated by magnetic cell sorting. Cells were cultured on a collagen matrix in a defined medium containing hepatocyte growth factor. Cell count and size were measured by flow cytometry, and reverse transcription polymerase chain reaction was carried out for the liver-specific markers CK-19 and albumin. During cell culture, CD34(+) cells showed an increasing cell number and proliferative activity as assessed by Ki-67 staining. Under the specified culture conditions, CD34(+) cells expressed albumin RNA and CK-19 RNA after 28 days, whereas CD34(-) cells did not show liver-specific gene expression. The results indicate that CD34(+) adult human bone marrow stem cells can differentiate into hepatocytic cells in vitro.

AB - Hematopoietic and hepatic stem cells share characteristic markers such as CD34, c-kit, and Thy1. Based on the recent observations that hepatocytes may originate from bone marrow, we investigated the potential of CD34(+) bone marrow cells to differentiate into hepatocytic cells in vitro. CD34(+) and CD34(-) human bone marrow cells were separated by magnetic cell sorting. Cells were cultured on a collagen matrix in a defined medium containing hepatocyte growth factor. Cell count and size were measured by flow cytometry, and reverse transcription polymerase chain reaction was carried out for the liver-specific markers CK-19 and albumin. During cell culture, CD34(+) cells showed an increasing cell number and proliferative activity as assessed by Ki-67 staining. Under the specified culture conditions, CD34(+) cells expressed albumin RNA and CK-19 RNA after 28 days, whereas CD34(-) cells did not show liver-specific gene expression. The results indicate that CD34(+) adult human bone marrow stem cells can differentiate into hepatocytic cells in vitro.

M3 - SCORING: Zeitschriftenaufsatz

VL - 21

SP - 98

EP - 104

JO - STEM CELLS

JF - STEM CELLS

SN - 1066-5099

IS - 1

M1 - 1

ER -