Live Cell Imaging of Cyclic Nucleotides in Human Cardiomyocytes.

Kira Beneke; Cristina Molina

doi:10.1007/978-1-0716-2245-2_12

Live Cell Imaging of Cyclic Nucleotides in Human Cardiomyocytes.

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Live Cell Imaging of Cyclic Nucleotides in Human Cardiomyocytes. / Beneke, Kira; Molina, Cristina.

cAMP Signaling: Methods and Protocols. Hrsg. / Manuela Zaccolo. 2. Aufl. Methods in Molecular Biology : Humana Press, 2022. S. 195-204 (Methods in Molecular Biology; Band 2483).

Publikationen: SCORING: Beitrag in Buch/Sammelwerk › Kapitel › Forschung › Begutachtung

Harvard

Beneke, K & Molina, C 2022, Live Cell Imaging of Cyclic Nucleotides in Human Cardiomyocytes. in M Zaccolo (Hrsg.), cAMP Signaling: Methods and Protocols. 2 Aufl., Methods in Molecular Biology, Bd. 2483, Humana Press, Methods in Molecular Biology, S. 195-204. https://doi.org/10.1007/978-1-0716-2245-2_12

APA

Beneke, K., & Molina, C. (2022). Live Cell Imaging of Cyclic Nucleotides in Human Cardiomyocytes. in M. Zaccolo (Hrsg.), cAMP Signaling: Methods and Protocols (2 Aufl., S. 195-204). (Methods in Molecular Biology; Band 2483). Humana Press. https://doi.org/10.1007/978-1-0716-2245-2_12

Vancouver

Beneke K, Molina C. Live Cell Imaging of Cyclic Nucleotides in Human Cardiomyocytes. in Zaccolo M, Hrsg., cAMP Signaling: Methods and Protocols. 2 Aufl. Methods in Molecular Biology: Humana Press. 2022. S. 195-204. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-0716-2245-2_12

Bibtex

@inbook{7e4fca43fff348b380d51d38a2b28b79,

title = "Live Cell Imaging of Cyclic Nucleotides in Human Cardiomyocytes.",

abstract = "The ubiquitous second messengers' 3',5'-cyclic adenosine monophosphate (cAMP ) and 3',5'-cyclic guanosine monophosphate (cGMP) are crucial in regulating cardiomyocyte function, as well as pathological processes, by acting in distinct subcellular microdomains and thus controlling excitation-contraction coupling. Spatio-temporal intracellular dynamics of cyclic nucleotides can be measured in living cells using fluorescence resonance energy transfer (FRET ) by transducing isolated cells with genetically encoded biosensors. While FRET experiments have been regularly performed in cardiomyocytes from different animal models, human-based translational experiments are very challenging due to the difficulty to culture and transduce adult human cardiomyocytes. Here, we describe a technique for obtaining human atrial and ventricular myocytes which allows to keep them alive in culture long enough to transduce them and visualize cAMP and cGMP in physiological and pathological human settings.Keywords: Atrial myocytes; Biosensors; Culture; Fluorescence resonance energy transfer; Human; Isolation; Ventricular myocytes; cAMP; cGMP.",

author = "Kira Beneke and Cristina Molina",

year = "2022",

doi = "10.1007/978-1-0716-2245-2_12",

language = "English",

isbn = "978-1-0716-2244-5",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "195--204",

editor = "Manuela Zaccolo",

booktitle = "cAMP Signaling",

address = "United States",

edition = "2",

}

RIS

TY - CHAP

T1 - Live Cell Imaging of Cyclic Nucleotides in Human Cardiomyocytes.

AU - Beneke, Kira

AU - Molina, Cristina

PY - 2022

Y1 - 2022

N2 - The ubiquitous second messengers' 3',5'-cyclic adenosine monophosphate (cAMP ) and 3',5'-cyclic guanosine monophosphate (cGMP) are crucial in regulating cardiomyocyte function, as well as pathological processes, by acting in distinct subcellular microdomains and thus controlling excitation-contraction coupling. Spatio-temporal intracellular dynamics of cyclic nucleotides can be measured in living cells using fluorescence resonance energy transfer (FRET ) by transducing isolated cells with genetically encoded biosensors. While FRET experiments have been regularly performed in cardiomyocytes from different animal models, human-based translational experiments are very challenging due to the difficulty to culture and transduce adult human cardiomyocytes. Here, we describe a technique for obtaining human atrial and ventricular myocytes which allows to keep them alive in culture long enough to transduce them and visualize cAMP and cGMP in physiological and pathological human settings.Keywords: Atrial myocytes; Biosensors; Culture; Fluorescence resonance energy transfer; Human; Isolation; Ventricular myocytes; cAMP; cGMP.

AB - The ubiquitous second messengers' 3',5'-cyclic adenosine monophosphate (cAMP ) and 3',5'-cyclic guanosine monophosphate (cGMP) are crucial in regulating cardiomyocyte function, as well as pathological processes, by acting in distinct subcellular microdomains and thus controlling excitation-contraction coupling. Spatio-temporal intracellular dynamics of cyclic nucleotides can be measured in living cells using fluorescence resonance energy transfer (FRET ) by transducing isolated cells with genetically encoded biosensors. While FRET experiments have been regularly performed in cardiomyocytes from different animal models, human-based translational experiments are very challenging due to the difficulty to culture and transduce adult human cardiomyocytes. Here, we describe a technique for obtaining human atrial and ventricular myocytes which allows to keep them alive in culture long enough to transduce them and visualize cAMP and cGMP in physiological and pathological human settings.Keywords: Atrial myocytes; Biosensors; Culture; Fluorescence resonance energy transfer; Human; Isolation; Ventricular myocytes; cAMP; cGMP.

U2 - 10.1007/978-1-0716-2245-2_12

DO - 10.1007/978-1-0716-2245-2_12

M3 - Chapter

SN - 978-1-0716-2244-5

T3 - Methods in Molecular Biology

SP - 195

EP - 204

BT - cAMP Signaling

A2 - Zaccolo, Manuela

PB - Humana Press

CY - Methods in Molecular Biology

ER -