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Liquid application method for time-resolved analyses by serial synchrotron crystallography

Standard

Liquid application method for time-resolved analyses by serial synchrotron crystallography. / Mehrabi, Pedram; Schulz, Eike C; Agthe, Michael; Horrell, Sam; Bourenkov, Gleb; von Stetten, David; Leimkohl, Jan-Philipp; Schikora, Hendrik; Schneider, Thomas R; Pearson, Arwen R; Tellkamp, Friedjof; Miller, R J Dwayne.

in: NAT METHODS, Jahrgang 16, Nr. 10, 10.2019, S. 979-982.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Mehrabi, P, Schulz, EC, Agthe, M, Horrell, S, Bourenkov, G, von Stetten, D, Leimkohl, J-P, Schikora, H, Schneider, TR, Pearson, AR, Tellkamp, F & Miller, RJD 2019, 'Liquid application method for time-resolved analyses by serial synchrotron crystallography', NAT METHODS, Jg. 16, Nr. 10, S. 979-982. https://doi.org/10.1038/s41592-019-0553-1

APA

Mehrabi, P., Schulz, E. C., Agthe, M., Horrell, S., Bourenkov, G., von Stetten, D., Leimkohl, J-P., Schikora, H., Schneider, T. R., Pearson, A. R., Tellkamp, F., & Miller, R. J. D. (2019). Liquid application method for time-resolved analyses by serial synchrotron crystallography. NAT METHODS, 16(10), 979-982. https://doi.org/10.1038/s41592-019-0553-1

Vancouver

Mehrabi P, Schulz EC, Agthe M, Horrell S, Bourenkov G, von Stetten D et al. Liquid application method for time-resolved analyses by serial synchrotron crystallography. NAT METHODS. 2019 Okt;16(10):979-982. https://doi.org/10.1038/s41592-019-0553-1

Bibtex

@article{3b5f6e5b281340d5a19306fb087a8ac8,
title = "Liquid application method for time-resolved analyses by serial synchrotron crystallography",
abstract = "We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.",
keywords = "Acetylglucosamine/chemistry, Aldose-Ketose Isomerases/chemistry, Crystallography/methods, Glucose/chemistry, Muramidase/chemistry, Proof of Concept Study, Synchrotrons",
author = "Pedram Mehrabi and Schulz, {Eike C} and Michael Agthe and Sam Horrell and Gleb Bourenkov and {von Stetten}, David and Jan-Philipp Leimkohl and Hendrik Schikora and Schneider, {Thomas R} and Pearson, {Arwen R} and Friedjof Tellkamp and Miller, {R J Dwayne}",
year = "2019",
month = oct,
doi = "10.1038/s41592-019-0553-1",
language = "English",
volume = "16",
pages = "979--982",
journal = "NAT METHODS",
issn = "1548-7091",
publisher = "NATURE PUBLISHING GROUP",
number = "10",

}

RIS

TY - JOUR

T1 - Liquid application method for time-resolved analyses by serial synchrotron crystallography

AU - Mehrabi, Pedram

AU - Schulz, Eike C

AU - Agthe, Michael

AU - Horrell, Sam

AU - Bourenkov, Gleb

AU - von Stetten, David

AU - Leimkohl, Jan-Philipp

AU - Schikora, Hendrik

AU - Schneider, Thomas R

AU - Pearson, Arwen R

AU - Tellkamp, Friedjof

AU - Miller, R J Dwayne

PY - 2019/10

Y1 - 2019/10

N2 - We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.

AB - We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.

KW - Acetylglucosamine/chemistry

KW - Aldose-Ketose Isomerases/chemistry

KW - Crystallography/methods

KW - Glucose/chemistry

KW - Muramidase/chemistry

KW - Proof of Concept Study

KW - Synchrotrons

U2 - 10.1038/s41592-019-0553-1

DO - 10.1038/s41592-019-0553-1

M3 - SCORING: Journal article

C2 - 31527838

VL - 16

SP - 979

EP - 982

JO - NAT METHODS

JF - NAT METHODS

SN - 1548-7091

IS - 10

ER -