LINE-1 retrotransposition events affect endothelial proliferation and migration
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LINE-1 retrotransposition events affect endothelial proliferation and migration. / Banaz-Yaşar, Ferya; Steffen, Gyde; Hauschild, Jessica; Bongartz, Birthe M; Schumann, Gerald G; Ergün, Süleyman.
in: HISTOCHEM CELL BIOL, Jahrgang 134, Nr. 6, 12.2010, S. 581-9.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - LINE-1 retrotransposition events affect endothelial proliferation and migration
AU - Banaz-Yaşar, Ferya
AU - Steffen, Gyde
AU - Hauschild, Jessica
AU - Bongartz, Birthe M
AU - Schumann, Gerald G
AU - Ergün, Süleyman
PY - 2010/12
Y1 - 2010/12
N2 - Long interspersed nuclear element-1 (LINE-1, L1) is a retrotransposon which affects the human genome by a variety of mechanisms. While LINE-1 expression is suppressed in the most somatic human cells, LINE-1 elements are activated in human cancer. Recently, high accumulation of LINE-1-encoded ORF1p and ORF2p in endothelial cells of mature human blood vessels was described. Here, we demonstrate that LINE-1 de novo retrotransposition events lead to a reduction of endothelial cell proliferation and migration in a porcine aortic endothelial (PAE) cell model. Cell cycle studies show a G0/G1 arrest in PAE cells harboring LINE-1 de novo retrotransposition events. Remarkably, in in situ analysis LINE-1-encoded ORF2p was not detectable in tumor blood vessels of different human organs while vascular endothelial cells of corresponding normal organs strongly expressed LINE-1 ORF2p. Quantitative RT-PCR analysis revealed that LINE-1 de novo retrotransposition influences selectively the expression of some angiogenic factors such as VEGF and Tie-2. Thus, our data suggest that LINE-1 de novo retrotransposition events might suppress angiogenesis and tumor vascularisation by reducing the angiogenic capacity of vascular endothelial cells.
AB - Long interspersed nuclear element-1 (LINE-1, L1) is a retrotransposon which affects the human genome by a variety of mechanisms. While LINE-1 expression is suppressed in the most somatic human cells, LINE-1 elements are activated in human cancer. Recently, high accumulation of LINE-1-encoded ORF1p and ORF2p in endothelial cells of mature human blood vessels was described. Here, we demonstrate that LINE-1 de novo retrotransposition events lead to a reduction of endothelial cell proliferation and migration in a porcine aortic endothelial (PAE) cell model. Cell cycle studies show a G0/G1 arrest in PAE cells harboring LINE-1 de novo retrotransposition events. Remarkably, in in situ analysis LINE-1-encoded ORF2p was not detectable in tumor blood vessels of different human organs while vascular endothelial cells of corresponding normal organs strongly expressed LINE-1 ORF2p. Quantitative RT-PCR analysis revealed that LINE-1 de novo retrotransposition influences selectively the expression of some angiogenic factors such as VEGF and Tie-2. Thus, our data suggest that LINE-1 de novo retrotransposition events might suppress angiogenesis and tumor vascularisation by reducing the angiogenic capacity of vascular endothelial cells.
KW - Animals
KW - Cell Movement
KW - Cell Proliferation
KW - Endonucleases
KW - Endothelium, Vascular
KW - Humans
KW - Ki-67 Antigen
KW - Long Interspersed Nucleotide Elements
KW - Male
KW - Neovascularization, Pathologic
KW - Prostatic Neoplasms
KW - RNA-Directed DNA Polymerase
KW - Receptor, TIE-2
KW - Swine
KW - Testicular Neoplasms
KW - Urinary Bladder Neoplasms
KW - Vascular Endothelial Growth Factor A
U2 - 10.1007/s00418-010-0758-y
DO - 10.1007/s00418-010-0758-y
M3 - SCORING: Journal article
C2 - 21069374
VL - 134
SP - 581
EP - 589
JO - HISTOCHEM CELL BIOL
JF - HISTOCHEM CELL BIOL
SN - 0948-6143
IS - 6
ER -
