Lectin histochemistry of resected adenocarcinoma of the lung

TY - JOUR

T1 - Lectin histochemistry of resected adenocarcinoma of the lung

T2 - helix pomatia agglutinin binding is an independent prognostic factor

AU - Laack, Eckart

AU - Nikbakht, Haleh

AU - Peters, Anja

AU - Kugler, Christian

AU - Jasiewicz, Yvonne

AU - Edler, Lutz

AU - Hossfeld, Dieter Kurt

AU - Schumacher, Udo

PY - 2002/3

Y1 - 2002/3

N2 - The worldwide incidence of adenocarcinoma of the lung is rising. Unfortunately, no significant prognostic marker beyond the classical TNM staging exists to stratify these patients for appropriate therapy. Because lectins, carbohydrate-binding proteins, have been shown to be useful prognostic markers in several other adenocarcinomas, a panel of lectins [Helix pomatia agglutinin (HPA), Phaseolus vulgaris leukoagglutinin, Ulex europaeus agglutinin, Maackia amurenis agglutinin, Sambucus nigra agglutinin] with different carbohydrate-binding specificities were tested for their prognostic relevance. Paraffin wax sections of 93 patients with adenocarcinomas of the lung who had undergone surgery between 1990 and 1995 were investigated by lectin histochemistry. Lectin-binding data and other known prognostic factors were correlated with survival. In univariate analysis, binding of HPA, Phaseolus vulgaris leukoagglutinin, and Ulex europaeus agglutinin to adenocarcinoma cells were prognostic indicators for overall and relapse-free survival, whereas Maackia amurenis agglutinin and Sambucus nigra agglutinin binding had no prognostic value. However, in a multivariate analysis next to stage and gender only HPA was a significant independent prognostic factor on survival. In conclusion, HPA binding was the primary marker-based predictor of prognosis in our patient population and allows to stratify patients with adenocarcinomas of the lung into a low- and a high-risk group.

AB - The worldwide incidence of adenocarcinoma of the lung is rising. Unfortunately, no significant prognostic marker beyond the classical TNM staging exists to stratify these patients for appropriate therapy. Because lectins, carbohydrate-binding proteins, have been shown to be useful prognostic markers in several other adenocarcinomas, a panel of lectins [Helix pomatia agglutinin (HPA), Phaseolus vulgaris leukoagglutinin, Ulex europaeus agglutinin, Maackia amurenis agglutinin, Sambucus nigra agglutinin] with different carbohydrate-binding specificities were tested for their prognostic relevance. Paraffin wax sections of 93 patients with adenocarcinomas of the lung who had undergone surgery between 1990 and 1995 were investigated by lectin histochemistry. Lectin-binding data and other known prognostic factors were correlated with survival. In univariate analysis, binding of HPA, Phaseolus vulgaris leukoagglutinin, and Ulex europaeus agglutinin to adenocarcinoma cells were prognostic indicators for overall and relapse-free survival, whereas Maackia amurenis agglutinin and Sambucus nigra agglutinin binding had no prognostic value. However, in a multivariate analysis next to stage and gender only HPA was a significant independent prognostic factor on survival. In conclusion, HPA binding was the primary marker-based predictor of prognosis in our patient population and allows to stratify patients with adenocarcinomas of the lung into a low- and a high-risk group.

KW - Adenocarcinoma

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Animals

KW - Female

KW - Helix (Snails)

KW - Histocytochemistry

KW - Humans

KW - Lectins

KW - Lung Neoplasms

KW - Male

KW - Middle Aged

KW - Predictive Value of Tests

KW - Prognosis

KW - Survival Analysis

KW - Tumor Markers, Biological

U2 - 10.1016/S0002-9440(10)64921-8

DO - 10.1016/S0002-9440(10)64921-8

M3 - SCORING: Journal article

C2 - 11891197

VL - 160

SP - 1001

EP - 1008

JO - AM J PATHOL

JF - AM J PATHOL

SN - 0002-9440

IS - 3

ER -