Lack of association between CTLA-4 and PDCD1 polymorphisms and acute rejection in German liver transplant recipients
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Lack of association between CTLA-4 and PDCD1 polymorphisms and acute rejection in German liver transplant recipients. / Thude, Hansjörg; Schipler, Anna Dorothea; Treszl, Andras; Peine, Sven; Koch, Martina; Sterneck, Martina; Nashan, Björn.
in: HUM IMMUNOL, Jahrgang 74, Nr. 8, 01.08.2013, S. 1041-5.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Lack of association between CTLA-4 and PDCD1 polymorphisms and acute rejection in German liver transplant recipients
AU - Thude, Hansjörg
AU - Schipler, Anna Dorothea
AU - Treszl, Andras
AU - Peine, Sven
AU - Koch, Martina
AU - Sterneck, Martina
AU - Nashan, Björn
N1 - Copyright © 2013 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
PY - 2013/8/1
Y1 - 2013/8/1
N2 - Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell-death 1 (PDCD1) are two genes encoding coinhibitory immunoreceptors that are involved in regulation transplant rejection and tolerance induction. Thus, CTLA-4 and PDCD1 may be good candidate genes to evaluate in liver transplant rejection. In this retrospective study, we investigated whether four functional single nucleotide polymorphisms (SNP) of the CTLA-4 gene and PDCD1 gene were associated with susceptibility to liver transplant rejection. The SNPs -1772T>C (rs733618), -1661A>G (rs4553808) of the CTLA-4 gene, and the SNPs 7146G>A (rs11568821), 7209C>T (rs41386349) of the PDCD1 gene were genotyped by polymerase chain reaction allele specific restriction enzyme analysis (PCR-ASRA) in 100 liver recipients with acute rejection, 104 liver transplant recipients without acute rejection and 100 healthy control individuals. For the selected SNPs we did not detect any significant difference in genotypic and allelic frequencies between liver transplant recipients with and without acute rejection. In conclusion, our results suggest that the tested SNPs may not be associated with susceptibility to acute liver transplant rejection in a Caucasian population.
AB - Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell-death 1 (PDCD1) are two genes encoding coinhibitory immunoreceptors that are involved in regulation transplant rejection and tolerance induction. Thus, CTLA-4 and PDCD1 may be good candidate genes to evaluate in liver transplant rejection. In this retrospective study, we investigated whether four functional single nucleotide polymorphisms (SNP) of the CTLA-4 gene and PDCD1 gene were associated with susceptibility to liver transplant rejection. The SNPs -1772T>C (rs733618), -1661A>G (rs4553808) of the CTLA-4 gene, and the SNPs 7146G>A (rs11568821), 7209C>T (rs41386349) of the PDCD1 gene were genotyped by polymerase chain reaction allele specific restriction enzyme analysis (PCR-ASRA) in 100 liver recipients with acute rejection, 104 liver transplant recipients without acute rejection and 100 healthy control individuals. For the selected SNPs we did not detect any significant difference in genotypic and allelic frequencies between liver transplant recipients with and without acute rejection. In conclusion, our results suggest that the tested SNPs may not be associated with susceptibility to acute liver transplant rejection in a Caucasian population.
KW - Adult
KW - Aged
KW - Alleles
KW - CTLA-4 Antigen
KW - European Continental Ancestry Group
KW - Female
KW - Gene Frequency
KW - Genotype
KW - Germany
KW - Graft Rejection
KW - Humans
KW - Liver Transplantation
KW - Male
KW - Middle Aged
KW - Odds Ratio
KW - Polymorphism, Single Nucleotide
KW - Programmed Cell Death 1 Receptor
KW - Retrospective Studies
U2 - 10.1016/j.humimm.2013.04.021
DO - 10.1016/j.humimm.2013.04.021
M3 - SCORING: Journal article
C2 - 23628397
VL - 74
SP - 1041
EP - 1045
JO - HUM IMMUNOL
JF - HUM IMMUNOL
SN - 0198-8859
IS - 8
ER -
