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Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells.

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Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. / Zeitz, Oliver; Schlichting, Lars; Richard, Gisbert; Strauss, Olaf.

in: GRAEF ARCH CLIN EXP, Jahrgang 245, Nr. 2, 2, 2007, S. 276-281.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Zeitz, O, Schlichting, L, Richard, G & Strauss, O 2007, 'Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells.', GRAEF ARCH CLIN EXP, Jg. 245, Nr. 2, 2, S. 276-281. <http://www.ncbi.nlm.nih.gov/pubmed/16868779?dopt=Citation>

APA

Zeitz, O., Schlichting, L., Richard, G., & Strauss, O. (2007). Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. GRAEF ARCH CLIN EXP, 245(2), 276-281. [2]. http://www.ncbi.nlm.nih.gov/pubmed/16868779?dopt=Citation

Vancouver

Zeitz O, Schlichting L, Richard G, Strauss O. Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. GRAEF ARCH CLIN EXP. 2007;245(2):276-281. 2.

Bibtex

@article{47d22deed85f455084f33a0e44e93fbd,
title = "Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells.",
abstract = "BACKGROUND: Oxidative damage to the retinal pigment epithelium might be involved in the pathogenesis of age related macular degeneration. Thus antioxidative protection represents a rationale for a causative therapy or prophylaxis. The aim of the present study is to evaluate antioxidative properties of vitamin C and pyruvate at retinal pigment epithelial (RPE) cells exposed to oxidative stress. METHODS: The ability of vitamin C and pyruvate to quench hydroxyl radicals was tested using the di-hydro-rhodamine (DHR) assay. Cells of the human RPE cell line ARPE-19 were exposed for 8 min to hydroxyl radicals generated by the Fenton reaction from 2.25 mM H2O2 and 30 microM Fe3+ -nitrilo-tri-acetate. This was done in the absence and presence of 0.3-3.0 mM pyruvate and vitamin C, respectively. Cell survival was analysed by vitality staining (life-dead-assay) and expressed as cell survival ratio. A survival ratio",
author = "Oliver Zeitz and Lars Schlichting and Gisbert Richard and Olaf Strauss",
year = "2007",
language = "Deutsch",
volume = "245",
pages = "276--281",
journal = "GRAEF ARCH CLIN EXP",
issn = "0721-832X",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells.

AU - Zeitz, Oliver

AU - Schlichting, Lars

AU - Richard, Gisbert

AU - Strauss, Olaf

PY - 2007

Y1 - 2007

N2 - BACKGROUND: Oxidative damage to the retinal pigment epithelium might be involved in the pathogenesis of age related macular degeneration. Thus antioxidative protection represents a rationale for a causative therapy or prophylaxis. The aim of the present study is to evaluate antioxidative properties of vitamin C and pyruvate at retinal pigment epithelial (RPE) cells exposed to oxidative stress. METHODS: The ability of vitamin C and pyruvate to quench hydroxyl radicals was tested using the di-hydro-rhodamine (DHR) assay. Cells of the human RPE cell line ARPE-19 were exposed for 8 min to hydroxyl radicals generated by the Fenton reaction from 2.25 mM H2O2 and 30 microM Fe3+ -nitrilo-tri-acetate. This was done in the absence and presence of 0.3-3.0 mM pyruvate and vitamin C, respectively. Cell survival was analysed by vitality staining (life-dead-assay) and expressed as cell survival ratio. A survival ratio

AB - BACKGROUND: Oxidative damage to the retinal pigment epithelium might be involved in the pathogenesis of age related macular degeneration. Thus antioxidative protection represents a rationale for a causative therapy or prophylaxis. The aim of the present study is to evaluate antioxidative properties of vitamin C and pyruvate at retinal pigment epithelial (RPE) cells exposed to oxidative stress. METHODS: The ability of vitamin C and pyruvate to quench hydroxyl radicals was tested using the di-hydro-rhodamine (DHR) assay. Cells of the human RPE cell line ARPE-19 were exposed for 8 min to hydroxyl radicals generated by the Fenton reaction from 2.25 mM H2O2 and 30 microM Fe3+ -nitrilo-tri-acetate. This was done in the absence and presence of 0.3-3.0 mM pyruvate and vitamin C, respectively. Cell survival was analysed by vitality staining (life-dead-assay) and expressed as cell survival ratio. A survival ratio

M3 - SCORING: Zeitschriftenaufsatz

VL - 245

SP - 276

EP - 281

JO - GRAEF ARCH CLIN EXP

JF - GRAEF ARCH CLIN EXP

SN - 0721-832X

IS - 2

M1 - 2

ER -