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Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy

Standard

Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy. / Ernst, Ann-Kathrin; Putscher, Annika; Samatov, Timur R; Suling, Anna; Galatenko, Vladimir V; Shkurnikov, Maxim Yu; Knyazev, Evgeny N; Tonevitsky, Alexander G; Haalck, Thomas; Lange, Tobias; Maar, Hanna; Schröder-Schwarz, Jennifer; Riecken, Kristoffer; Schumacher, Udo; Wicklein, Daniel.

in: PLOS ONE, Jahrgang 13, Nr. 2, 2018, S. e0192525.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ernst, A-K, Putscher, A, Samatov, TR, Suling, A, Galatenko, VV, Shkurnikov, MY, Knyazev, EN, Tonevitsky, AG, Haalck, T, Lange, T, Maar, H, Schröder-Schwarz, J, Riecken, K, Schumacher, U & Wicklein, D 2018, 'Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy', PLOS ONE, Jg. 13, Nr. 2, S. e0192525. https://doi.org/10.1371/journal.pone.0192525

APA

Ernst, A-K., Putscher, A., Samatov, T. R., Suling, A., Galatenko, V. V., Shkurnikov, M. Y., Knyazev, E. N., Tonevitsky, A. G., Haalck, T., Lange, T., Maar, H., Schröder-Schwarz, J., Riecken, K., Schumacher, U., & Wicklein, D. (2018). Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy. PLOS ONE, 13(2), e0192525. https://doi.org/10.1371/journal.pone.0192525

Vancouver

Ernst A-K, Putscher A, Samatov TR, Suling A, Galatenko VV, Shkurnikov MY et al. Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy. PLOS ONE. 2018;13(2):e0192525. https://doi.org/10.1371/journal.pone.0192525

Bibtex

@article{4b36dc4f903148d3b7cfdcfd19be1b34,
title = "Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy",
abstract = "Finding additional functional targets for combination therapy could improve the outcome for melanoma patients. In a spontaneous metastasis xenograft model of human melanoma a shRNA mediated knockdown of L1CAM more than sevenfold reduced the number of lung metastases after the induction of subcutaneous tumors for two human melanoma cell lines (MeWo, MV3). Whole genome expression arrays of the initially L1CAM high MeWo subcutaneous tumors revealed unchanged or downregulated genes involved in epithelial to mesenchymal transition (EMT) except an upregulation of Jagged 1, indicating a compensatory change in Notch signaling especially as Jagged 1 expression showed an increase in MeWo L1CAM metastases and Jagged 1 was expressed in metastases of the initially L1CAM low MV3 cells as well. Expression of 17 genes showed concordant regulation for L1CAM knockdown tumors of both cell lines. The changes in gene expression indicated changes in the EMT network of the melanoma cells and an increase in p53/p21 and p38 activity contributing to the reduced metastatic potential of the L1CAM knockdowns. Taken together, these data make L1CAM a highly interesting therapeutic target to prevent further metastatic spread in melanoma patients.",
keywords = "Journal Article",
author = "Ann-Kathrin Ernst and Annika Putscher and Samatov, {Timur R} and Anna Suling and Galatenko, {Vladimir V} and Shkurnikov, {Maxim Yu} and Knyazev, {Evgeny N} and Tonevitsky, {Alexander G} and Thomas Haalck and Tobias Lange and Hanna Maar and Jennifer Schr{\"o}der-Schwarz and Kristoffer Riecken and Udo Schumacher and Daniel Wicklein",
year = "2018",
doi = "10.1371/journal.pone.0192525",
language = "English",
volume = "13",
pages = "e0192525",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

RIS

TY - JOUR

T1 - Knockdown of L1CAM significantly reduces metastasis in a xenograft model of human melanoma: L1CAM is a potential target for anti-melanoma therapy

AU - Ernst, Ann-Kathrin

AU - Putscher, Annika

AU - Samatov, Timur R

AU - Suling, Anna

AU - Galatenko, Vladimir V

AU - Shkurnikov, Maxim Yu

AU - Knyazev, Evgeny N

AU - Tonevitsky, Alexander G

AU - Haalck, Thomas

AU - Lange, Tobias

AU - Maar, Hanna

AU - Schröder-Schwarz, Jennifer

AU - Riecken, Kristoffer

AU - Schumacher, Udo

AU - Wicklein, Daniel

PY - 2018

Y1 - 2018

N2 - Finding additional functional targets for combination therapy could improve the outcome for melanoma patients. In a spontaneous metastasis xenograft model of human melanoma a shRNA mediated knockdown of L1CAM more than sevenfold reduced the number of lung metastases after the induction of subcutaneous tumors for two human melanoma cell lines (MeWo, MV3). Whole genome expression arrays of the initially L1CAM high MeWo subcutaneous tumors revealed unchanged or downregulated genes involved in epithelial to mesenchymal transition (EMT) except an upregulation of Jagged 1, indicating a compensatory change in Notch signaling especially as Jagged 1 expression showed an increase in MeWo L1CAM metastases and Jagged 1 was expressed in metastases of the initially L1CAM low MV3 cells as well. Expression of 17 genes showed concordant regulation for L1CAM knockdown tumors of both cell lines. The changes in gene expression indicated changes in the EMT network of the melanoma cells and an increase in p53/p21 and p38 activity contributing to the reduced metastatic potential of the L1CAM knockdowns. Taken together, these data make L1CAM a highly interesting therapeutic target to prevent further metastatic spread in melanoma patients.

AB - Finding additional functional targets for combination therapy could improve the outcome for melanoma patients. In a spontaneous metastasis xenograft model of human melanoma a shRNA mediated knockdown of L1CAM more than sevenfold reduced the number of lung metastases after the induction of subcutaneous tumors for two human melanoma cell lines (MeWo, MV3). Whole genome expression arrays of the initially L1CAM high MeWo subcutaneous tumors revealed unchanged or downregulated genes involved in epithelial to mesenchymal transition (EMT) except an upregulation of Jagged 1, indicating a compensatory change in Notch signaling especially as Jagged 1 expression showed an increase in MeWo L1CAM metastases and Jagged 1 was expressed in metastases of the initially L1CAM low MV3 cells as well. Expression of 17 genes showed concordant regulation for L1CAM knockdown tumors of both cell lines. The changes in gene expression indicated changes in the EMT network of the melanoma cells and an increase in p53/p21 and p38 activity contributing to the reduced metastatic potential of the L1CAM knockdowns. Taken together, these data make L1CAM a highly interesting therapeutic target to prevent further metastatic spread in melanoma patients.

KW - Journal Article

U2 - 10.1371/journal.pone.0192525

DO - 10.1371/journal.pone.0192525

M3 - SCORING: Journal article

C2 - 29432466

VL - 13

SP - e0192525

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 2

ER -