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KIBRA modulates directional migration of podocytes

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KIBRA modulates directional migration of podocytes. / Duning, Kerstin; Schurek, Eva-Maria; Schlüter, Marc; Bayer, Michael; Reinhardt, Hans-Christian; Schwab, Albrecht; Schaefer, Liliana; Benzing, Thomas; Schermer, Bernhard; Saleem, Moin A; Huber, Tobias B; Bachmann, Sebastian; Kremerskothen, Joachim; Weide, Thomas; Pavenstädt, Hermann.

in: J AM SOC NEPHROL, Jahrgang 19, Nr. 10, 10.2008, S. 1891-903.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Duning, K, Schurek, E-M, Schlüter, M, Bayer, M, Reinhardt, H-C, Schwab, A, Schaefer, L, Benzing, T, Schermer, B, Saleem, MA, Huber, TB, Bachmann, S, Kremerskothen, J, Weide, T & Pavenstädt, H 2008, 'KIBRA modulates directional migration of podocytes', J AM SOC NEPHROL, Jg. 19, Nr. 10, S. 1891-903. https://doi.org/10.1681/ASN.2007080916

APA

Duning, K., Schurek, E-M., Schlüter, M., Bayer, M., Reinhardt, H-C., Schwab, A., Schaefer, L., Benzing, T., Schermer, B., Saleem, M. A., Huber, T. B., Bachmann, S., Kremerskothen, J., Weide, T., & Pavenstädt, H. (2008). KIBRA modulates directional migration of podocytes. J AM SOC NEPHROL, 19(10), 1891-903. https://doi.org/10.1681/ASN.2007080916

Vancouver

Duning K, Schurek E-M, Schlüter M, Bayer M, Reinhardt H-C, Schwab A et al. KIBRA modulates directional migration of podocytes. J AM SOC NEPHROL. 2008 Okt;19(10):1891-903. https://doi.org/10.1681/ASN.2007080916

Bibtex

@article{07c102ed78c54f7f9618f1facb092a8c,
title = "KIBRA modulates directional migration of podocytes",
abstract = "Asymmetric delivery and distribution of macromolecules are essential for cell polarity and for cellular functions such as differentiation, division, and signaling. Injury of podocytes, which are polarized epithelial cells, changes the dynamics of the actin meshwork, resulting in foot process retraction and proteinuria. Although the spatiotemporal control of specific protein-protein interactions is crucial for the establishment of cell polarity, the mechanisms controlling polarity-dependent differentiation and division are incompletely understood. In this study, yeast two-hybrid screens were performed using a podocyte cDNA library and the polarity protein PATJ as bait. The protein KIBRA was identified as an interaction partner of PATJ and was localized to podocytes, tubular structures, and collecting ducts. The last four amino acids of KIBRA mediated binding to the eighth PDZ domain of PATJ. In addition, KIBRA directly bound to synaptopodin, an essential organizer of the podocyte cytoskeleton. Stable knockdown of KIBRA in immortalized podocytes impaired directed cell migration, suggesting that KIBRA modulates the motility of podocytes by linking polarity proteins and cytoskeleton-associated protein complexes.",
keywords = "Cell Culture Techniques, Cell Movement, Cell Polarity, Cytoskeletal Proteins, Gene Library, Humans, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Phosphoproteins, Podocytes, Protein Binding, Proteins, RNA, Messenger, Tight Junction Proteins, Two-Hybrid System Techniques, Journal Article, Research Support, Non-U.S. Gov't",
author = "Kerstin Duning and Eva-Maria Schurek and Marc Schl{\"u}ter and Michael Bayer and Hans-Christian Reinhardt and Albrecht Schwab and Liliana Schaefer and Thomas Benzing and Bernhard Schermer and Saleem, {Moin A} and Huber, {Tobias B} and Sebastian Bachmann and Joachim Kremerskothen and Thomas Weide and Hermann Pavenst{\"a}dt",
year = "2008",
month = oct,
doi = "10.1681/ASN.2007080916",
language = "English",
volume = "19",
pages = "1891--903",
journal = "J AM SOC NEPHROL",
issn = "1046-6673",
publisher = "American Society of Nephrology",
number = "10",

}

RIS

TY - JOUR

T1 - KIBRA modulates directional migration of podocytes

AU - Duning, Kerstin

AU - Schurek, Eva-Maria

AU - Schlüter, Marc

AU - Bayer, Michael

AU - Reinhardt, Hans-Christian

AU - Schwab, Albrecht

AU - Schaefer, Liliana

AU - Benzing, Thomas

AU - Schermer, Bernhard

AU - Saleem, Moin A

AU - Huber, Tobias B

AU - Bachmann, Sebastian

AU - Kremerskothen, Joachim

AU - Weide, Thomas

AU - Pavenstädt, Hermann

PY - 2008/10

Y1 - 2008/10

N2 - Asymmetric delivery and distribution of macromolecules are essential for cell polarity and for cellular functions such as differentiation, division, and signaling. Injury of podocytes, which are polarized epithelial cells, changes the dynamics of the actin meshwork, resulting in foot process retraction and proteinuria. Although the spatiotemporal control of specific protein-protein interactions is crucial for the establishment of cell polarity, the mechanisms controlling polarity-dependent differentiation and division are incompletely understood. In this study, yeast two-hybrid screens were performed using a podocyte cDNA library and the polarity protein PATJ as bait. The protein KIBRA was identified as an interaction partner of PATJ and was localized to podocytes, tubular structures, and collecting ducts. The last four amino acids of KIBRA mediated binding to the eighth PDZ domain of PATJ. In addition, KIBRA directly bound to synaptopodin, an essential organizer of the podocyte cytoskeleton. Stable knockdown of KIBRA in immortalized podocytes impaired directed cell migration, suggesting that KIBRA modulates the motility of podocytes by linking polarity proteins and cytoskeleton-associated protein complexes.

AB - Asymmetric delivery and distribution of macromolecules are essential for cell polarity and for cellular functions such as differentiation, division, and signaling. Injury of podocytes, which are polarized epithelial cells, changes the dynamics of the actin meshwork, resulting in foot process retraction and proteinuria. Although the spatiotemporal control of specific protein-protein interactions is crucial for the establishment of cell polarity, the mechanisms controlling polarity-dependent differentiation and division are incompletely understood. In this study, yeast two-hybrid screens were performed using a podocyte cDNA library and the polarity protein PATJ as bait. The protein KIBRA was identified as an interaction partner of PATJ and was localized to podocytes, tubular structures, and collecting ducts. The last four amino acids of KIBRA mediated binding to the eighth PDZ domain of PATJ. In addition, KIBRA directly bound to synaptopodin, an essential organizer of the podocyte cytoskeleton. Stable knockdown of KIBRA in immortalized podocytes impaired directed cell migration, suggesting that KIBRA modulates the motility of podocytes by linking polarity proteins and cytoskeleton-associated protein complexes.

KW - Cell Culture Techniques

KW - Cell Movement

KW - Cell Polarity

KW - Cytoskeletal Proteins

KW - Gene Library

KW - Humans

KW - Intracellular Signaling Peptides and Proteins

KW - Membrane Proteins

KW - Phosphoproteins

KW - Podocytes

KW - Protein Binding

KW - Proteins

KW - RNA, Messenger

KW - Tight Junction Proteins

KW - Two-Hybrid System Techniques

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1681/ASN.2007080916

DO - 10.1681/ASN.2007080916

M3 - SCORING: Journal article

C2 - 18596123

VL - 19

SP - 1891

EP - 1903

JO - J AM SOC NEPHROL

JF - J AM SOC NEPHROL

SN - 1046-6673

IS - 10

ER -