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KCNMA1 gene amplification promotes tumor cell proliferation in human prostate cancer.

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KCNMA1 gene amplification promotes tumor cell proliferation in human prostate cancer. / Bloch, M; Ousingsawat, J; Simon, Ronald; Schraml, P; Gasser, T C; Mihatsch, M J; Kunzelmann, K; Bubendorf, L.

in: ONCOGENE, Jahrgang 26, Nr. 17, 17, 2007, S. 2525-2534.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Bloch, M, Ousingsawat, J, Simon, R, Schraml, P, Gasser, TC, Mihatsch, MJ, Kunzelmann, K & Bubendorf, L 2007, 'KCNMA1 gene amplification promotes tumor cell proliferation in human prostate cancer.', ONCOGENE, Jg. 26, Nr. 17, 17, S. 2525-2534. <http://www.ncbi.nlm.nih.gov/pubmed/17146446?dopt=Citation>

APA

Bloch, M., Ousingsawat, J., Simon, R., Schraml, P., Gasser, T. C., Mihatsch, M. J., Kunzelmann, K., & Bubendorf, L. (2007). KCNMA1 gene amplification promotes tumor cell proliferation in human prostate cancer. ONCOGENE, 26(17), 2525-2534. [17]. http://www.ncbi.nlm.nih.gov/pubmed/17146446?dopt=Citation

Vancouver

Bloch M, Ousingsawat J, Simon R, Schraml P, Gasser TC, Mihatsch MJ et al. KCNMA1 gene amplification promotes tumor cell proliferation in human prostate cancer. ONCOGENE. 2007;26(17):2525-2534. 17.

Bibtex

@article{2f258d97cbbc43809eb82defa98bdc5b,
title = "KCNMA1 gene amplification promotes tumor cell proliferation in human prostate cancer.",
abstract = "Molecular mechanisms of prostate cancer progression are poorly understood. Here, we studied gene amplification of the large conductance calcium-activated potassium channel alpha subunit (KCNMA1), which is located at the chromosomal region 10q22. Fluorescence in situ hybridization (FISH) revealed KCNMA1 amplification in 16% of 119 late-stage human prostate cancers and in the hormone-insensitive prostate cancer cell line PC-3. In contrast, KCNMA1 amplification was absent in 33 benign controls, 32 precursor lesions and in 105 clinically organ-confined prostate cancers. Amplification was associated with mRNA and protein overexpression as well as increased density of BK channel protein and beta-estradiol-insensitive BK currents in PC-3 cells as compared to non-amplified control cell lines. Specific blockade of BK channels by iberiotoxin or RNA(i) significantly inhibited K(+) currents and growth of PC-3 cells. The data demonstrate that 10q22 amplification drives KCNMA1 expression and cell proliferation. Thus, KCNMA1 qualifies as a promising diagnostic and therapeutic target in patients with prostate cancer.",
author = "M Bloch and J Ousingsawat and Ronald Simon and P Schraml and Gasser, {T C} and Mihatsch, {M J} and K Kunzelmann and L Bubendorf",
year = "2007",
language = "Deutsch",
volume = "26",
pages = "2525--2534",
journal = "ONCOGENE",
issn = "0950-9232",
publisher = "NATURE PUBLISHING GROUP",
number = "17",

}

RIS

TY - JOUR

T1 - KCNMA1 gene amplification promotes tumor cell proliferation in human prostate cancer.

AU - Bloch, M

AU - Ousingsawat, J

AU - Simon, Ronald

AU - Schraml, P

AU - Gasser, T C

AU - Mihatsch, M J

AU - Kunzelmann, K

AU - Bubendorf, L

PY - 2007

Y1 - 2007

N2 - Molecular mechanisms of prostate cancer progression are poorly understood. Here, we studied gene amplification of the large conductance calcium-activated potassium channel alpha subunit (KCNMA1), which is located at the chromosomal region 10q22. Fluorescence in situ hybridization (FISH) revealed KCNMA1 amplification in 16% of 119 late-stage human prostate cancers and in the hormone-insensitive prostate cancer cell line PC-3. In contrast, KCNMA1 amplification was absent in 33 benign controls, 32 precursor lesions and in 105 clinically organ-confined prostate cancers. Amplification was associated with mRNA and protein overexpression as well as increased density of BK channel protein and beta-estradiol-insensitive BK currents in PC-3 cells as compared to non-amplified control cell lines. Specific blockade of BK channels by iberiotoxin or RNA(i) significantly inhibited K(+) currents and growth of PC-3 cells. The data demonstrate that 10q22 amplification drives KCNMA1 expression and cell proliferation. Thus, KCNMA1 qualifies as a promising diagnostic and therapeutic target in patients with prostate cancer.

AB - Molecular mechanisms of prostate cancer progression are poorly understood. Here, we studied gene amplification of the large conductance calcium-activated potassium channel alpha subunit (KCNMA1), which is located at the chromosomal region 10q22. Fluorescence in situ hybridization (FISH) revealed KCNMA1 amplification in 16% of 119 late-stage human prostate cancers and in the hormone-insensitive prostate cancer cell line PC-3. In contrast, KCNMA1 amplification was absent in 33 benign controls, 32 precursor lesions and in 105 clinically organ-confined prostate cancers. Amplification was associated with mRNA and protein overexpression as well as increased density of BK channel protein and beta-estradiol-insensitive BK currents in PC-3 cells as compared to non-amplified control cell lines. Specific blockade of BK channels by iberiotoxin or RNA(i) significantly inhibited K(+) currents and growth of PC-3 cells. The data demonstrate that 10q22 amplification drives KCNMA1 expression and cell proliferation. Thus, KCNMA1 qualifies as a promising diagnostic and therapeutic target in patients with prostate cancer.

M3 - SCORING: Zeitschriftenaufsatz

VL - 26

SP - 2525

EP - 2534

JO - ONCOGENE

JF - ONCOGENE

SN - 0950-9232

IS - 17

M1 - 17

ER -