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Itinerary of hepatitis B viruses: delineation of restriction points critical for infectious entry.

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Itinerary of hepatitis B viruses: delineation of restriction points critical for infectious entry. / Funk, Anneke; Mouna, Mhamdi; Lin, Li; Will, Hans; Sirma, Hüseyin.

in: J VIROL, Jahrgang 78, Nr. 15, 15, 2004, S. 8289-8300.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Funk, A, Mouna, M, Lin, L, Will, H & Sirma, H 2004, 'Itinerary of hepatitis B viruses: delineation of restriction points critical for infectious entry.', J VIROL, Jg. 78, Nr. 15, 15, S. 8289-8300. <http://www.ncbi.nlm.nih.gov/pubmed/15254201?dopt=Citation>

APA

Funk, A., Mouna, M., Lin, L., Will, H., & Sirma, H. (2004). Itinerary of hepatitis B viruses: delineation of restriction points critical for infectious entry. J VIROL, 78(15), 8289-8300. [15]. http://www.ncbi.nlm.nih.gov/pubmed/15254201?dopt=Citation

Vancouver

Funk A, Mouna M, Lin L, Will H, Sirma H. Itinerary of hepatitis B viruses: delineation of restriction points critical for infectious entry. J VIROL. 2004;78(15):8289-8300. 15.

Bibtex

@article{860b9303b4f74e27aa2f3562bf3e3dd0,
title = "Itinerary of hepatitis B viruses: delineation of restriction points critical for infectious entry.",
abstract = "Little is known about cellular determinants essential for human hepatitis B virus infection. Using the duck hepatitis B virus as a model, we first established a sensitive binding assay for both virions and subviral particles and subsequently elucidated the characteristics of the early viral entry steps. The infection itinerary was found to initiate with the attachment of viral particles to a low number of binding sites on hepatocytes (about 10(4) per cell). Virus internalization was fully accomplished in less than 3 h but was then followed by a period of unprecedented length, about 14 h, until completion of nuclear import of the viral genome. Steps subsequent to virus entry depended on both intact microtubules and their dynamic turnover but not on actin cytoskeleton. Notably, cytoplasmic trafficking of viral particles and emergence of nuclear covalently closed circular DNA requires microtubules during entry only at and for specific time periods. Taken together, these data disclose for the first time a series of steps and their kinetics that are essential for the entry of hepatitis B viruses into hepatocytes and are different from those of any other virus reported so far.",
author = "Anneke Funk and Mhamdi Mouna and Li Lin and Hans Will and H{\"u}seyin Sirma",
year = "2004",
language = "Deutsch",
volume = "78",
pages = "8289--8300",
journal = "J VIROL",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "15",

}

RIS

TY - JOUR

T1 - Itinerary of hepatitis B viruses: delineation of restriction points critical for infectious entry.

AU - Funk, Anneke

AU - Mouna, Mhamdi

AU - Lin, Li

AU - Will, Hans

AU - Sirma, Hüseyin

PY - 2004

Y1 - 2004

N2 - Little is known about cellular determinants essential for human hepatitis B virus infection. Using the duck hepatitis B virus as a model, we first established a sensitive binding assay for both virions and subviral particles and subsequently elucidated the characteristics of the early viral entry steps. The infection itinerary was found to initiate with the attachment of viral particles to a low number of binding sites on hepatocytes (about 10(4) per cell). Virus internalization was fully accomplished in less than 3 h but was then followed by a period of unprecedented length, about 14 h, until completion of nuclear import of the viral genome. Steps subsequent to virus entry depended on both intact microtubules and their dynamic turnover but not on actin cytoskeleton. Notably, cytoplasmic trafficking of viral particles and emergence of nuclear covalently closed circular DNA requires microtubules during entry only at and for specific time periods. Taken together, these data disclose for the first time a series of steps and their kinetics that are essential for the entry of hepatitis B viruses into hepatocytes and are different from those of any other virus reported so far.

AB - Little is known about cellular determinants essential for human hepatitis B virus infection. Using the duck hepatitis B virus as a model, we first established a sensitive binding assay for both virions and subviral particles and subsequently elucidated the characteristics of the early viral entry steps. The infection itinerary was found to initiate with the attachment of viral particles to a low number of binding sites on hepatocytes (about 10(4) per cell). Virus internalization was fully accomplished in less than 3 h but was then followed by a period of unprecedented length, about 14 h, until completion of nuclear import of the viral genome. Steps subsequent to virus entry depended on both intact microtubules and their dynamic turnover but not on actin cytoskeleton. Notably, cytoplasmic trafficking of viral particles and emergence of nuclear covalently closed circular DNA requires microtubules during entry only at and for specific time periods. Taken together, these data disclose for the first time a series of steps and their kinetics that are essential for the entry of hepatitis B viruses into hepatocytes and are different from those of any other virus reported so far.

M3 - SCORING: Zeitschriftenaufsatz

VL - 78

SP - 8289

EP - 8300

JO - J VIROL

JF - J VIROL

SN - 0022-538X

IS - 15

M1 - 15

ER -