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In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity

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In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity. / Halle, Stephan; Keyser, Kirsten Anja; Stahl, Felix Rolf; Busche, Andreas; Marquardt, Anja; Zheng, Xiang; Galla, Melanie; Heissmeyer, Vigo; Heller, Katrin; Boelter, Jasmin; Wagner, Karen; Bischoff, Yvonne; Martens, Rieke; Braun, Asolina; Werth, Kathrin; Uvarovskii, Alexey; Kempf, Harald; Meyer-Hermann, Michael; Arens, Ramon; Kremer, Melanie; Sutter, Gerd; Messerle, Martin; Förster, Reinhold.

in: IMMUNITY, Jahrgang 44, Nr. 2, 16.02.2016, S. 233-45.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Halle, S, Keyser, KA, Stahl, FR, Busche, A, Marquardt, A, Zheng, X, Galla, M, Heissmeyer, V, Heller, K, Boelter, J, Wagner, K, Bischoff, Y, Martens, R, Braun, A, Werth, K, Uvarovskii, A, Kempf, H, Meyer-Hermann, M, Arens, R, Kremer, M, Sutter, G, Messerle, M & Förster, R 2016, 'In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity', IMMUNITY, Jg. 44, Nr. 2, S. 233-45. https://doi.org/10.1016/j.immuni.2016.01.010

APA

Halle, S., Keyser, K. A., Stahl, F. R., Busche, A., Marquardt, A., Zheng, X., Galla, M., Heissmeyer, V., Heller, K., Boelter, J., Wagner, K., Bischoff, Y., Martens, R., Braun, A., Werth, K., Uvarovskii, A., Kempf, H., Meyer-Hermann, M., Arens, R., ... Förster, R. (2016). In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity. IMMUNITY, 44(2), 233-45. https://doi.org/10.1016/j.immuni.2016.01.010

Vancouver

Halle S, Keyser KA, Stahl FR, Busche A, Marquardt A, Zheng X et al. In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity. IMMUNITY. 2016 Feb 16;44(2):233-45. https://doi.org/10.1016/j.immuni.2016.01.010

Bibtex

@article{76882d3ec59b4d00b7d6b5731fb82ac0,
title = "In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity",
abstract = "According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated killing of virus-infected cells in vivo remains elusive. We used two-photon microscopy to quantify CTL-mediated killing in mice infected with herpesviruses or poxviruses. On average, one CTL killed 2-16 virus-infected cells per day as determined by real-time imaging and by mathematical modeling. In contrast, upon virus-induced MHC class I downmodulation, CTLs failed to destroy their targets. During killing, CTLs remained migratory and formed motile kinapses rather than static synapses with targets. Viruses encoding the calcium sensor GCaMP6s revealed strong heterogeneity in individual CTL functional capacity. Furthermore, the probability of death of infected cells increased for those contacted by more than two CTLs, indicative of CTL cooperation. Thus, direct visualization of CTLs during killing of virus-infected cells reveals crucial parameters of CD8(+) T cell immunity.",
author = "Stephan Halle and Keyser, {Kirsten Anja} and Stahl, {Felix Rolf} and Andreas Busche and Anja Marquardt and Xiang Zheng and Melanie Galla and Vigo Heissmeyer and Katrin Heller and Jasmin Boelter and Karen Wagner and Yvonne Bischoff and Rieke Martens and Asolina Braun and Kathrin Werth and Alexey Uvarovskii and Harald Kempf and Michael Meyer-Hermann and Ramon Arens and Melanie Kremer and Gerd Sutter and Martin Messerle and Reinhold F{\"o}rster",
note = "Copyright {\textcopyright} 2016 Elsevier Inc. All rights reserved.",
year = "2016",
month = feb,
day = "16",
doi = "10.1016/j.immuni.2016.01.010",
language = "English",
volume = "44",
pages = "233--45",
journal = "IMMUNITY",
issn = "1074-7613",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity

AU - Halle, Stephan

AU - Keyser, Kirsten Anja

AU - Stahl, Felix Rolf

AU - Busche, Andreas

AU - Marquardt, Anja

AU - Zheng, Xiang

AU - Galla, Melanie

AU - Heissmeyer, Vigo

AU - Heller, Katrin

AU - Boelter, Jasmin

AU - Wagner, Karen

AU - Bischoff, Yvonne

AU - Martens, Rieke

AU - Braun, Asolina

AU - Werth, Kathrin

AU - Uvarovskii, Alexey

AU - Kempf, Harald

AU - Meyer-Hermann, Michael

AU - Arens, Ramon

AU - Kremer, Melanie

AU - Sutter, Gerd

AU - Messerle, Martin

AU - Förster, Reinhold

N1 - Copyright © 2016 Elsevier Inc. All rights reserved.

PY - 2016/2/16

Y1 - 2016/2/16

N2 - According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated killing of virus-infected cells in vivo remains elusive. We used two-photon microscopy to quantify CTL-mediated killing in mice infected with herpesviruses or poxviruses. On average, one CTL killed 2-16 virus-infected cells per day as determined by real-time imaging and by mathematical modeling. In contrast, upon virus-induced MHC class I downmodulation, CTLs failed to destroy their targets. During killing, CTLs remained migratory and formed motile kinapses rather than static synapses with targets. Viruses encoding the calcium sensor GCaMP6s revealed strong heterogeneity in individual CTL functional capacity. Furthermore, the probability of death of infected cells increased for those contacted by more than two CTLs, indicative of CTL cooperation. Thus, direct visualization of CTLs during killing of virus-infected cells reveals crucial parameters of CD8(+) T cell immunity.

AB - According to in vitro assays, T cells are thought to kill rapidly and efficiently, but the efficacy and dynamics of cytotoxic T lymphocyte (CTL)-mediated killing of virus-infected cells in vivo remains elusive. We used two-photon microscopy to quantify CTL-mediated killing in mice infected with herpesviruses or poxviruses. On average, one CTL killed 2-16 virus-infected cells per day as determined by real-time imaging and by mathematical modeling. In contrast, upon virus-induced MHC class I downmodulation, CTLs failed to destroy their targets. During killing, CTLs remained migratory and formed motile kinapses rather than static synapses with targets. Viruses encoding the calcium sensor GCaMP6s revealed strong heterogeneity in individual CTL functional capacity. Furthermore, the probability of death of infected cells increased for those contacted by more than two CTLs, indicative of CTL cooperation. Thus, direct visualization of CTLs during killing of virus-infected cells reveals crucial parameters of CD8(+) T cell immunity.

U2 - 10.1016/j.immuni.2016.01.010

DO - 10.1016/j.immuni.2016.01.010

M3 - SCORING: Journal article

C2 - 26872694

VL - 44

SP - 233

EP - 245

JO - IMMUNITY

JF - IMMUNITY

SN - 1074-7613

IS - 2

ER -