Investigation of Viral and Host Chromatin by ChIP-PCR or ChIP-Seq Analysis
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Investigation of Viral and Host Chromatin by ChIP-PCR or ChIP-Seq Analysis. / Günther, Thomas; Theiss, Juliane M; Fischer, Nicole; Grundhoff, Adam.
in: Curr Protoc Microbiol, Jahrgang 40, 2016, S. 1E.10.1-1E.10.21.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Investigation of Viral and Host Chromatin by ChIP-PCR or ChIP-Seq Analysis
AU - Günther, Thomas
AU - Theiss, Juliane M
AU - Fischer, Nicole
AU - Grundhoff, Adam
N1 - Copyright © 2016 John Wiley & Sons, Inc.
PY - 2016
Y1 - 2016
N2 - Complex regulation of viral transcription patterns and DNA replication levels is a feature of many DNA viruses. This is especially true for those viruses which establish latent or persistent infections (e.g., herpesviruses, papillomaviruses, polyomaviruses, or adenovirus), as long-term persistence often requires adaptation of gene expression programs and/or replication levels to the cellular milieu. A key factor in the control of such processes is the establishment of a specific chromatin state on promoters or replication origins, which in turn will determine whether or not the underlying DNA is accessible for other factors that mediate downstream processes. Chromatin immunoprecipitation (ChIP) is a powerful technique to investigate viral chromatin, in particular to study binding patterns of modified histones, transcription factors or other DNA-/chromatin-binding proteins that regulate the viral lifecycle. Here, we provide protocols that are suitable for performing ChIP-PCR and ChIP-Seq studies on chromatin of large and small viral genomes.© 2016 by John Wiley & Sons, Inc.
AB - Complex regulation of viral transcription patterns and DNA replication levels is a feature of many DNA viruses. This is especially true for those viruses which establish latent or persistent infections (e.g., herpesviruses, papillomaviruses, polyomaviruses, or adenovirus), as long-term persistence often requires adaptation of gene expression programs and/or replication levels to the cellular milieu. A key factor in the control of such processes is the establishment of a specific chromatin state on promoters or replication origins, which in turn will determine whether or not the underlying DNA is accessible for other factors that mediate downstream processes. Chromatin immunoprecipitation (ChIP) is a powerful technique to investigate viral chromatin, in particular to study binding patterns of modified histones, transcription factors or other DNA-/chromatin-binding proteins that regulate the viral lifecycle. Here, we provide protocols that are suitable for performing ChIP-PCR and ChIP-Seq studies on chromatin of large and small viral genomes.© 2016 by John Wiley & Sons, Inc.
U2 - 10.1002/9780471729259.mc01e10s40
DO - 10.1002/9780471729259.mc01e10s40
M3 - SCORING: Journal article
C2 - 26855283
VL - 40
SP - 1E.10.1-1E.10.21
JO - Curr Protoc Microbiol
JF - Curr Protoc Microbiol
SN - 1934-8525
ER -
