Intra- and intercompartmental movement of gammadelta T cells
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Intra- and intercompartmental movement of gammadelta T cells : intestinal intraepithelial and peripheral gammadelta T cells represent exclusive nonoverlapping populations with distinct migration characteristics. / Chennupati, Vijaykumar; Worbs, Tim; Liu, Xiaosun; Malinarich, Frano H; Schmitz, Susanne; Haas, Jan D; Malissen, Bernard; Förster, Reinhold; Prinz, Immo.
in: J IMMUNOL, Jahrgang 185, Nr. 9, 01.11.2010, S. 5160-8.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Intra- and intercompartmental movement of gammadelta T cells
T2 - intestinal intraepithelial and peripheral gammadelta T cells represent exclusive nonoverlapping populations with distinct migration characteristics
AU - Chennupati, Vijaykumar
AU - Worbs, Tim
AU - Liu, Xiaosun
AU - Malinarich, Frano H
AU - Schmitz, Susanne
AU - Haas, Jan D
AU - Malissen, Bernard
AU - Förster, Reinhold
AU - Prinz, Immo
PY - 2010/11/1
Y1 - 2010/11/1
N2 - Unlike the ∼1% of γδ TCR-positive T cells being regularly present in blood and secondary lymphoid organs (peripheral γδ T cells), ∼50-60% of small intestinal intraepithelial lymphocytes (iIELs) in the mouse express the γδ TCR (γδ iIELs). In this study, we investigated the overlap and exchange of γδ iIELs and γδ T cells found in peripheral secondary lymphoid organs. Using two-photon laser-scanning microscopy, we found γδ T cells within peripheral lymph nodes to be highly motile, whereas γδ iIELs were characterized by a locally confined scanning behavior. Our results implied a strict separation of peripheral γδ T cells and γδ iIELs. Nevertheless, γδ iIELs could be efficiently regenerated from bone marrow-derived precursors in irradiated or T cell-deficient adult mice. However, outside the intestinal epithelium, survival of γδ iIELs was very poor. In CCR9-deficient mice, homing of γδ iIELs was impaired, but did not lead to an accumulation of γδ iIEL-like cells in the periphery. Conversely, in situations in which specific γδ iIEL niches were empty, adoptive transfer of isolated γδ iIELs led to a sustained engraftment of transferred γδ iIELs in the intestinal epithelium for at least 100 d. Furthermore, we demonstrated by heterotopic intestinal transplantation experiments that an exchange of γδ iIELs only rarely happens in the steady state of adult mice. We therefore conclude that peripheral versus intestinal intraepithelial γδ T cells are exclusive, nonoverlapping populations that virtually do not exchange with each other.
AB - Unlike the ∼1% of γδ TCR-positive T cells being regularly present in blood and secondary lymphoid organs (peripheral γδ T cells), ∼50-60% of small intestinal intraepithelial lymphocytes (iIELs) in the mouse express the γδ TCR (γδ iIELs). In this study, we investigated the overlap and exchange of γδ iIELs and γδ T cells found in peripheral secondary lymphoid organs. Using two-photon laser-scanning microscopy, we found γδ T cells within peripheral lymph nodes to be highly motile, whereas γδ iIELs were characterized by a locally confined scanning behavior. Our results implied a strict separation of peripheral γδ T cells and γδ iIELs. Nevertheless, γδ iIELs could be efficiently regenerated from bone marrow-derived precursors in irradiated or T cell-deficient adult mice. However, outside the intestinal epithelium, survival of γδ iIELs was very poor. In CCR9-deficient mice, homing of γδ iIELs was impaired, but did not lead to an accumulation of γδ iIEL-like cells in the periphery. Conversely, in situations in which specific γδ iIEL niches were empty, adoptive transfer of isolated γδ iIELs led to a sustained engraftment of transferred γδ iIELs in the intestinal epithelium for at least 100 d. Furthermore, we demonstrated by heterotopic intestinal transplantation experiments that an exchange of γδ iIELs only rarely happens in the steady state of adult mice. We therefore conclude that peripheral versus intestinal intraepithelial γδ T cells are exclusive, nonoverlapping populations that virtually do not exchange with each other.
KW - Adoptive Transfer
KW - Animals
KW - Cell Lineage/immunology
KW - Cell Movement/immunology
KW - Cell Separation
KW - Flow Cytometry
KW - Immunity, Mucosal/immunology
KW - Intestinal Mucosa/cytology
KW - Lymph Nodes/cytology
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Knockout
KW - Microscopy, Confocal
KW - Receptors, Antigen, T-Cell, gamma-delta/immunology
KW - T-Lymphocyte Subsets/cytology
KW - T-Lymphocytes/cytology
U2 - 10.4049/jimmunol.1001652
DO - 10.4049/jimmunol.1001652
M3 - SCORING: Journal article
C2 - 20870939
VL - 185
SP - 5160
EP - 5168
JO - J IMMUNOL
JF - J IMMUNOL
SN - 0022-1767
IS - 9
ER -