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Interaction of magnetically labeled multipotent mesenchymal stromal cells and E-and P-selectins monitored by magnetic resonance imaging in mice

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Interaction of magnetically labeled multipotent mesenchymal stromal cells and E-and P-selectins monitored by magnetic resonance imaging in mice. / Peldschus, Kersten; Salamon, Johannes; Wicklein, Daniel; Lange, Claudia; Ittrich, Harald; Adam, Gerhard; Schumacher, Udo.

in: MOL IMAGING, Jahrgang 12, Nr. 2, 2013, S. 100-10.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Peldschus, K, Salamon, J, Wicklein, D, Lange, C, Ittrich, H, Adam, G & Schumacher, U 2013, 'Interaction of magnetically labeled multipotent mesenchymal stromal cells and E-and P-selectins monitored by magnetic resonance imaging in mice', MOL IMAGING, Jg. 12, Nr. 2, S. 100-10.

APA

Peldschus, K., Salamon, J., Wicklein, D., Lange, C., Ittrich, H., Adam, G., & Schumacher, U. (2013). Interaction of magnetically labeled multipotent mesenchymal stromal cells and E-and P-selectins monitored by magnetic resonance imaging in mice. MOL IMAGING, 12(2), 100-10.

Vancouver

Peldschus K, Salamon J, Wicklein D, Lange C, Ittrich H, Adam G et al. Interaction of magnetically labeled multipotent mesenchymal stromal cells and E-and P-selectins monitored by magnetic resonance imaging in mice. MOL IMAGING. 2013;12(2):100-10.

Bibtex

@article{99873bb3fb01493491067d24d815bb00,
title = "Interaction of magnetically labeled multipotent mesenchymal stromal cells and E-and P-selectins monitored by magnetic resonance imaging in mice",
abstract = "The purpose of this study was to analyze the influence of E- and P-selectins on the migratory pattern of magnetically labeled multipotent mesenchymal stromal cells (MSCs) in mice using magnetic resonance imaging (MRI). Murine MSCs were labeled with fluorescent iron oxide microparticles and carboxyfluorescein succinidyl ester (CFSE). Expression of common MSC markers, CD44, CD90, CD105, and Sca-1, as well as of selectin ligands, CD15s and CD162, was assessed using flow cytometry and immunocytochemistry. Labeled MSCs were injected into E-/P-selectin-deficient and wild-type mice applying doses of 5 × 10(4) cells intracardially, 1 × 10(6) cells intravenously, and 5 × 10(6) cells intraperitoneally. After cell administration, mice underwent MRI repeatedly and histologic evaluation after 7 days. The results demonstrate that magnetically labeled murine MSCs retain their expression of the above-mentioned surface markers and their ability to interact with P-selectin. Furthermore, MRI examinations and histologic analysis revealed that E-/P-selectin deficiency in mice significantly alters the distribution of labeled MSCs after cell injection via different routes.",
keywords = "Animals, Cells, Cultured, E-Selectin, Flow Cytometry, Immunohistochemistry, Iron, Magnetic Resonance Imaging, Male, Mesenchymal Stromal Cells, Mice, Mice, Inbred C57BL, P-Selectin",
author = "Kersten Peldschus and Johannes Salamon and Daniel Wicklein and Claudia Lange and Harald Ittrich and Gerhard Adam and Udo Schumacher",
year = "2013",
language = "English",
volume = "12",
pages = "100--10",
journal = "MOL IMAGING",
issn = "1535-3508",
publisher = "Decker Publishing",
number = "2",

}

RIS

TY - JOUR

T1 - Interaction of magnetically labeled multipotent mesenchymal stromal cells and E-and P-selectins monitored by magnetic resonance imaging in mice

AU - Peldschus, Kersten

AU - Salamon, Johannes

AU - Wicklein, Daniel

AU - Lange, Claudia

AU - Ittrich, Harald

AU - Adam, Gerhard

AU - Schumacher, Udo

PY - 2013

Y1 - 2013

N2 - The purpose of this study was to analyze the influence of E- and P-selectins on the migratory pattern of magnetically labeled multipotent mesenchymal stromal cells (MSCs) in mice using magnetic resonance imaging (MRI). Murine MSCs were labeled with fluorescent iron oxide microparticles and carboxyfluorescein succinidyl ester (CFSE). Expression of common MSC markers, CD44, CD90, CD105, and Sca-1, as well as of selectin ligands, CD15s and CD162, was assessed using flow cytometry and immunocytochemistry. Labeled MSCs were injected into E-/P-selectin-deficient and wild-type mice applying doses of 5 × 10(4) cells intracardially, 1 × 10(6) cells intravenously, and 5 × 10(6) cells intraperitoneally. After cell administration, mice underwent MRI repeatedly and histologic evaluation after 7 days. The results demonstrate that magnetically labeled murine MSCs retain their expression of the above-mentioned surface markers and their ability to interact with P-selectin. Furthermore, MRI examinations and histologic analysis revealed that E-/P-selectin deficiency in mice significantly alters the distribution of labeled MSCs after cell injection via different routes.

AB - The purpose of this study was to analyze the influence of E- and P-selectins on the migratory pattern of magnetically labeled multipotent mesenchymal stromal cells (MSCs) in mice using magnetic resonance imaging (MRI). Murine MSCs were labeled with fluorescent iron oxide microparticles and carboxyfluorescein succinidyl ester (CFSE). Expression of common MSC markers, CD44, CD90, CD105, and Sca-1, as well as of selectin ligands, CD15s and CD162, was assessed using flow cytometry and immunocytochemistry. Labeled MSCs were injected into E-/P-selectin-deficient and wild-type mice applying doses of 5 × 10(4) cells intracardially, 1 × 10(6) cells intravenously, and 5 × 10(6) cells intraperitoneally. After cell administration, mice underwent MRI repeatedly and histologic evaluation after 7 days. The results demonstrate that magnetically labeled murine MSCs retain their expression of the above-mentioned surface markers and their ability to interact with P-selectin. Furthermore, MRI examinations and histologic analysis revealed that E-/P-selectin deficiency in mice significantly alters the distribution of labeled MSCs after cell injection via different routes.

KW - Animals

KW - Cells, Cultured

KW - E-Selectin

KW - Flow Cytometry

KW - Immunohistochemistry

KW - Iron

KW - Magnetic Resonance Imaging

KW - Male

KW - Mesenchymal Stromal Cells

KW - Mice

KW - Mice, Inbred C57BL

KW - P-Selectin

M3 - SCORING: Journal article

C2 - 23415398

VL - 12

SP - 100

EP - 110

JO - MOL IMAGING

JF - MOL IMAGING

SN - 1535-3508

IS - 2

ER -