Integration and differentiation of neural stem cells after transplantation into the dysmyelinated central nervous system of adult mice

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Integration and differentiation of neural stem cells after transplantation into the dysmyelinated central nervous system of adult mice. / Ader, Marius; Schachner, Melitta; Bartsch, Udo.

in: EUR J NEUROSCI, Jahrgang 20, Nr. 5, 09.2004, S. 1205-10.

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@article{eec34ed4e9ca43b68b5433cd4e973efb,
title = "Integration and differentiation of neural stem cells after transplantation into the dysmyelinated central nervous system of adult mice",
abstract = "Mutant mice deficient in the myelin-associated glycoprotein (MAG) and the nonreceptor-type tyrosine kinase Fyn are characterized by a severely hypomyelinated central nervous system (CNS) and morphologically abnormal myelin sheaths. Despite this pronounced phenotype, MAG/Fyn-deficient mice have a normal longevity. In the present study, we took advantage of the normal life expectancy of this myelin mutant and grafted neural stem cells (NSCs) into the CNS of MAG/Fyn-deficient mice to study in short- and long-term experiments the fate of NSCs in adult dysmyelinated brains. Neural stem cells were isolated from spinal cords of transgenic mouse embryos ubiquitously expressing enhanced green fluorescent protein. Cells were expanded in vitro in the presence of mitogens for up to 5 weeks before they were grafted into the lateral ventricles or injected into white matter tracts. Analysis of mutant brains 3-15 weeks after intracerebroventricular transplantation of NSCs revealed only limited integration of donor cells into the host brains. However, injection of NSCs directly into white matter tracts resulted in widespread distribution of donor cells within the host tissue. Donor cells survived for at least 15 weeks in adult host brains. The majority of grafted cells populated white matter tracts and differentiated into oligodendrocytes that myelinated host axons. Results suggest that intraparenchymal transplantation of NSCs might be a strategy to reconstruct myelin in dysmyelinated adult brains.",
keywords = "Animals, Brain, Brain Tissue Transplantation, Cell Differentiation, Cells, Cultured, Demyelinating Autoimmune Diseases, CNS, Female, Fetal Tissue Transplantation, Mice, Mice, Transgenic, Myelin-Associated Glycoprotein, Neurons, Pregnancy, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-fyn, Stem Cell Transplantation, Comparative Study, Journal Article",
author = "Marius Ader and Melitta Schachner and Udo Bartsch",
year = "2004",
month = sep,
doi = "10.1111/j.1460-9568.2004.03577.x",
language = "English",
volume = "20",
pages = "1205--10",
journal = "EUR J NEUROSCI",
issn = "0953-816X",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Integration and differentiation of neural stem cells after transplantation into the dysmyelinated central nervous system of adult mice

AU - Ader, Marius

AU - Schachner, Melitta

AU - Bartsch, Udo

PY - 2004/9

Y1 - 2004/9

N2 - Mutant mice deficient in the myelin-associated glycoprotein (MAG) and the nonreceptor-type tyrosine kinase Fyn are characterized by a severely hypomyelinated central nervous system (CNS) and morphologically abnormal myelin sheaths. Despite this pronounced phenotype, MAG/Fyn-deficient mice have a normal longevity. In the present study, we took advantage of the normal life expectancy of this myelin mutant and grafted neural stem cells (NSCs) into the CNS of MAG/Fyn-deficient mice to study in short- and long-term experiments the fate of NSCs in adult dysmyelinated brains. Neural stem cells were isolated from spinal cords of transgenic mouse embryos ubiquitously expressing enhanced green fluorescent protein. Cells were expanded in vitro in the presence of mitogens for up to 5 weeks before they were grafted into the lateral ventricles or injected into white matter tracts. Analysis of mutant brains 3-15 weeks after intracerebroventricular transplantation of NSCs revealed only limited integration of donor cells into the host brains. However, injection of NSCs directly into white matter tracts resulted in widespread distribution of donor cells within the host tissue. Donor cells survived for at least 15 weeks in adult host brains. The majority of grafted cells populated white matter tracts and differentiated into oligodendrocytes that myelinated host axons. Results suggest that intraparenchymal transplantation of NSCs might be a strategy to reconstruct myelin in dysmyelinated adult brains.

AB - Mutant mice deficient in the myelin-associated glycoprotein (MAG) and the nonreceptor-type tyrosine kinase Fyn are characterized by a severely hypomyelinated central nervous system (CNS) and morphologically abnormal myelin sheaths. Despite this pronounced phenotype, MAG/Fyn-deficient mice have a normal longevity. In the present study, we took advantage of the normal life expectancy of this myelin mutant and grafted neural stem cells (NSCs) into the CNS of MAG/Fyn-deficient mice to study in short- and long-term experiments the fate of NSCs in adult dysmyelinated brains. Neural stem cells were isolated from spinal cords of transgenic mouse embryos ubiquitously expressing enhanced green fluorescent protein. Cells were expanded in vitro in the presence of mitogens for up to 5 weeks before they were grafted into the lateral ventricles or injected into white matter tracts. Analysis of mutant brains 3-15 weeks after intracerebroventricular transplantation of NSCs revealed only limited integration of donor cells into the host brains. However, injection of NSCs directly into white matter tracts resulted in widespread distribution of donor cells within the host tissue. Donor cells survived for at least 15 weeks in adult host brains. The majority of grafted cells populated white matter tracts and differentiated into oligodendrocytes that myelinated host axons. Results suggest that intraparenchymal transplantation of NSCs might be a strategy to reconstruct myelin in dysmyelinated adult brains.

KW - Animals

KW - Brain

KW - Brain Tissue Transplantation

KW - Cell Differentiation

KW - Cells, Cultured

KW - Demyelinating Autoimmune Diseases, CNS

KW - Female

KW - Fetal Tissue Transplantation

KW - Mice

KW - Mice, Transgenic

KW - Myelin-Associated Glycoprotein

KW - Neurons

KW - Pregnancy

KW - Proto-Oncogene Proteins

KW - Proto-Oncogene Proteins c-fyn

KW - Stem Cell Transplantation

KW - Comparative Study

KW - Journal Article

U2 - 10.1111/j.1460-9568.2004.03577.x

DO - 10.1111/j.1460-9568.2004.03577.x

M3 - SCORING: Journal article

C2 - 15341592

VL - 20

SP - 1205

EP - 1210

JO - EUR J NEUROSCI

JF - EUR J NEUROSCI

SN - 0953-816X

IS - 5

ER -