Insulin-like factor 3 serum levels in 135 normal men and 85 men with testicular disorders: relationship to the luteinizing hormone-testosterone axis

K Bay; S Hartung; R Ivell; M Schumacher; D Jürgensen; N Jorgensen; M Holm; N E Skakkebaek; A-M Andersson

doi:10.1210/jc.2004-2257

Insulin-like factor 3 serum levels in 135 normal men and 85 men with testicular disorders

Standard

Insulin-like factor 3 serum levels in 135 normal men and 85 men with testicular disorders : relationship to the luteinizing hormone-testosterone axis. / Bay, K; Hartung, S; Ivell, R; Schumacher, M; Jürgensen, D; Jorgensen, N; Holm, M; Skakkebaek, N E; Andersson, A-M.

in: J CLIN ENDOCR METAB, Jahrgang 90, Nr. 6, 01.06.2005, S. 3410-8.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Bay, K, Hartung, S, Ivell, R, Schumacher, M, Jürgensen, D, Jorgensen, N, Holm, M, Skakkebaek, NE & Andersson, A-M 2005, 'Insulin-like factor 3 serum levels in 135 normal men and 85 men with testicular disorders: relationship to the luteinizing hormone-testosterone axis', J CLIN ENDOCR METAB, Jg. 90, Nr. 6, S. 3410-8. https://doi.org/10.1210/jc.2004-2257

APA

Bay, K., Hartung, S., Ivell, R., Schumacher, M., Jürgensen, D., Jorgensen, N., Holm, M., Skakkebaek, N. E., & Andersson, A-M. (2005). Insulin-like factor 3 serum levels in 135 normal men and 85 men with testicular disorders: relationship to the luteinizing hormone-testosterone axis. J CLIN ENDOCR METAB, 90(6), 3410-8. https://doi.org/10.1210/jc.2004-2257

Vancouver

Bay K, Hartung S, Ivell R, Schumacher M, Jürgensen D, Jorgensen N et al. Insulin-like factor 3 serum levels in 135 normal men and 85 men with testicular disorders: relationship to the luteinizing hormone-testosterone axis. J CLIN ENDOCR METAB. 2005 Jun 1;90(6):3410-8. https://doi.org/10.1210/jc.2004-2257

Bibtex

@article{18818be27ab8468eae094b296eaf420c,

title = "Insulin-like factor 3 serum levels in 135 normal men and 85 men with testicular disorders: relationship to the luteinizing hormone-testosterone axis",

abstract = "Insulin-like factor 3 (INSL3) serum levels were measured in 135 andrologically well-characterized normal men and 85 patients with testicular disorders to investigate how the hormone, which is a major secretory product of human Leydig cells, is related to testosterone (T), LH, and semen quality. INSL3 was measured by using a newly developed fluorescence immunoassay. Median (2.5-97.5 percentiles) INSL3 serum levels were as follows: normal men (n = 135), 0.99 (0.55-1.73) ng/ml; infertile men (n = 23), 1.11 (0.60-2.07) ng/ml; anorchid men (n = 21), nondetectable (ND); patients with 47, XXY, Klinefelter syndrome (n = 21), 0.12 (ND-0.78) ng/ml; men with hypogonadotropic hypogonadism and T substitution (n = 11), ND; and men with hypogonadotropic hypogonadism and human chorionic gonadotropin (hCG) treatment (n = 5), 0.36 (0.13-0.73) ng/ml. Before testicular biopsy, two infertile men had blood samples drawn directly from vena spermatica. Here, the serum INSL3 levels were 15-fold higher than in serum from peripheral blood samples (13.84 and 14.00 ng/ml, respectively). In two unilaterally orchiectomized former testis cancer patients, who underwent hCG stimulation test, INSL3 serum levels were unchanged 72 and 96 h after hCG stimulation. In conclusion, we provide a normal range for INSL3 serum levels in adult men and show that the majority, if not all, circulating INSL3 derives from the testes. Furthermore, our data strongly indicate that INSL3 secretion is dependent on the differentiating effect of LH on Leydig cells but independent of the steroidogenic LH-mediated action. Thus, even though T and INSL3 are both dependent on LH, these two Leydig cell hormones are regulated differently.",

keywords = "Adolescent, Follicle Stimulating Hormone, Humans, Inhibins, Insulin, Luteinizing Hormone, Male, Middle Aged, Proteins, Reference Values, Semen, Sperm Count, Sperm Motility, Testicular Diseases, Testis, Testosterone",

author = "K Bay and S Hartung and R Ivell and M Schumacher and D J{\"u}rgensen and N Jorgensen and M Holm and Skakkebaek, {N E} and A-M Andersson",

year = "2005",

month = jun,

day = "1",

doi = "10.1210/jc.2004-2257",

language = "English",

volume = "90",

pages = "3410--8",

journal = "J CLIN ENDOCR METAB",

issn = "0021-972X",

publisher = "The Endocrine Society",

number = "6",

}

RIS

TY - JOUR

T1 - Insulin-like factor 3 serum levels in 135 normal men and 85 men with testicular disorders

T2 - relationship to the luteinizing hormone-testosterone axis

AU - Bay, K

AU - Hartung, S

AU - Ivell, R

AU - Schumacher, M

AU - Jürgensen, D

AU - Jorgensen, N

AU - Holm, M

AU - Skakkebaek, N E

AU - Andersson, A-M

PY - 2005/6/1

Y1 - 2005/6/1

N2 - Insulin-like factor 3 (INSL3) serum levels were measured in 135 andrologically well-characterized normal men and 85 patients with testicular disorders to investigate how the hormone, which is a major secretory product of human Leydig cells, is related to testosterone (T), LH, and semen quality. INSL3 was measured by using a newly developed fluorescence immunoassay. Median (2.5-97.5 percentiles) INSL3 serum levels were as follows: normal men (n = 135), 0.99 (0.55-1.73) ng/ml; infertile men (n = 23), 1.11 (0.60-2.07) ng/ml; anorchid men (n = 21), nondetectable (ND); patients with 47, XXY, Klinefelter syndrome (n = 21), 0.12 (ND-0.78) ng/ml; men with hypogonadotropic hypogonadism and T substitution (n = 11), ND; and men with hypogonadotropic hypogonadism and human chorionic gonadotropin (hCG) treatment (n = 5), 0.36 (0.13-0.73) ng/ml. Before testicular biopsy, two infertile men had blood samples drawn directly from vena spermatica. Here, the serum INSL3 levels were 15-fold higher than in serum from peripheral blood samples (13.84 and 14.00 ng/ml, respectively). In two unilaterally orchiectomized former testis cancer patients, who underwent hCG stimulation test, INSL3 serum levels were unchanged 72 and 96 h after hCG stimulation. In conclusion, we provide a normal range for INSL3 serum levels in adult men and show that the majority, if not all, circulating INSL3 derives from the testes. Furthermore, our data strongly indicate that INSL3 secretion is dependent on the differentiating effect of LH on Leydig cells but independent of the steroidogenic LH-mediated action. Thus, even though T and INSL3 are both dependent on LH, these two Leydig cell hormones are regulated differently.

AB - Insulin-like factor 3 (INSL3) serum levels were measured in 135 andrologically well-characterized normal men and 85 patients with testicular disorders to investigate how the hormone, which is a major secretory product of human Leydig cells, is related to testosterone (T), LH, and semen quality. INSL3 was measured by using a newly developed fluorescence immunoassay. Median (2.5-97.5 percentiles) INSL3 serum levels were as follows: normal men (n = 135), 0.99 (0.55-1.73) ng/ml; infertile men (n = 23), 1.11 (0.60-2.07) ng/ml; anorchid men (n = 21), nondetectable (ND); patients with 47, XXY, Klinefelter syndrome (n = 21), 0.12 (ND-0.78) ng/ml; men with hypogonadotropic hypogonadism and T substitution (n = 11), ND; and men with hypogonadotropic hypogonadism and human chorionic gonadotropin (hCG) treatment (n = 5), 0.36 (0.13-0.73) ng/ml. Before testicular biopsy, two infertile men had blood samples drawn directly from vena spermatica. Here, the serum INSL3 levels were 15-fold higher than in serum from peripheral blood samples (13.84 and 14.00 ng/ml, respectively). In two unilaterally orchiectomized former testis cancer patients, who underwent hCG stimulation test, INSL3 serum levels were unchanged 72 and 96 h after hCG stimulation. In conclusion, we provide a normal range for INSL3 serum levels in adult men and show that the majority, if not all, circulating INSL3 derives from the testes. Furthermore, our data strongly indicate that INSL3 secretion is dependent on the differentiating effect of LH on Leydig cells but independent of the steroidogenic LH-mediated action. Thus, even though T and INSL3 are both dependent on LH, these two Leydig cell hormones are regulated differently.

KW - Adolescent

KW - Follicle Stimulating Hormone

KW - Humans

KW - Inhibins

KW - Insulin

KW - Luteinizing Hormone

KW - Male

KW - Middle Aged

KW - Proteins

KW - Reference Values

KW - Semen

KW - Sperm Count

KW - Sperm Motility

KW - Testicular Diseases

KW - Testis

KW - Testosterone

U2 - 10.1210/jc.2004-2257

DO - 10.1210/jc.2004-2257

M3 - SCORING: Journal article

C2 - 15755855

VL - 90

SP - 3410

EP - 3418

JO - J CLIN ENDOCR METAB

JF - J CLIN ENDOCR METAB

SN - 0021-972X

IS - 6

ER -