Inhibition of hyaluronan export attenuates cell migration and metastasis of human melanoma.
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Inhibition of hyaluronan export attenuates cell migration and metastasis of human melanoma. / Monz, Katharina; Maas-Kück, Kathrin; Schumacher, Udo; Schulz, Tobias; Hallmann, Rupert; Schnäker, Eva Maria; Schneider, Stefan W; Prehm, Peter.
in: J CELL BIOCHEM, Jahrgang 105, Nr. 5, 5, 2008, S. 1260-1266.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Inhibition of hyaluronan export attenuates cell migration and metastasis of human melanoma.
AU - Monz, Katharina
AU - Maas-Kück, Kathrin
AU - Schumacher, Udo
AU - Schulz, Tobias
AU - Hallmann, Rupert
AU - Schnäker, Eva Maria
AU - Schneider, Stefan W
AU - Prehm, Peter
PY - 2008
Y1 - 2008
N2 - When secreted from malignant cells, hyaluronan facilitates tumor invasion and metastasis, as inhibition of its export by zaprinast inhibited metastasis formation in mice. However, the precise steps of the metastatic cascade, which were influenced by zaprinast, have not been identified as yet. Here we analyzed the cell biological effects of the inhibitor on three human melanoma cell lines that differed in their hyaluronan production and their metastatic capability when xenografted into SCID mice. We measured the influence of zaprinast on cellular hyaluronan export, surface coat formation, proliferation, random migration, colony formation in soft agar, adhesion, and transepithelial resistance. Concentrations of zaprinast not affecting cell proliferation, adhesion and transepithelial resistance, nevertheless reduced hyaluronan export by 50%, surface coat formation, random migration, and colony formation in soft agar. These results indicate that hyaluronan enhances metastasis formation primarily in those steps of the metastatic cascade, which involves tumor cell migration.
AB - When secreted from malignant cells, hyaluronan facilitates tumor invasion and metastasis, as inhibition of its export by zaprinast inhibited metastasis formation in mice. However, the precise steps of the metastatic cascade, which were influenced by zaprinast, have not been identified as yet. Here we analyzed the cell biological effects of the inhibitor on three human melanoma cell lines that differed in their hyaluronan production and their metastatic capability when xenografted into SCID mice. We measured the influence of zaprinast on cellular hyaluronan export, surface coat formation, proliferation, random migration, colony formation in soft agar, adhesion, and transepithelial resistance. Concentrations of zaprinast not affecting cell proliferation, adhesion and transepithelial resistance, nevertheless reduced hyaluronan export by 50%, surface coat formation, random migration, and colony formation in soft agar. These results indicate that hyaluronan enhances metastasis formation primarily in those steps of the metastatic cascade, which involves tumor cell migration.
M3 - SCORING: Zeitschriftenaufsatz
VL - 105
SP - 1260
EP - 1266
JO - J CELL BIOCHEM
JF - J CELL BIOCHEM
SN - 0730-2312
IS - 5
M1 - 5
ER -
